Özlem İzci Ay Özlem İzci Ay TIP FAKÜLTESİ TEMEL TIP BİLİMLERİ BÖLÜMÜ TIBBİ BİYOLOJİ ANABİLİM DALI
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Effects of electromagnetic radiation on neurogenesis and gene expression in amniocytes

Erdal, Nurten | Ay, Özlem İzci | Yıldırım, Didem Derici | Durukan, Hüseyin | Erdal, Mehmet Emin

The aim of the study was to investigate the genetic effects of commonly used radiofrequency on the amniocytes of pregnant women by examining the changes in the expression of genes related to signal transduction and neurogenesis induced by electromagnetic fields of radiofrequencies of 1800 and 2100 MHz by real-time PCR. Upon exposure of the amniocytes to electromagnetic radiation of 2100 MHz at high field strength, the expression levels of genes with important functions in the Wnt signaling pathways as well as neurogenesis-related genes were increased. The findings suggest that radiofrequency at high field strengths can have genotoxic effects during pregnancy.

Effects of electromagnetic radiation on neurogenesis and gene expression in amniocytes,

Erdal, Nurten | Ay, Özlem İzci | Yıldırım, Didem Derici | Durukan, Hüseyin | Erdal, Mehmet Emin

The aim of the study was to investigate the genetic effects of commonly used radiofrequency on the amniocytes of pregnant women by examining the changes in the expression of genes related to signal transduction and neurogenesis induced by electromagnetic fields of radiofrequencies of 1800 and 2100 MHz by real-time PCR. Upon exposure of the amniocytes to electromagnetic radiation of 2100 MHz at high field strength, the expression levels of genes with important functions in the Wnt signaling pathways as well as neurogenesis-related genes were increased. The findings suggest that radiofrequency at high field strengths can have genotoxic effects during pregnancy.

White matter alterations related to attention-deficit hyperactivity disorder and COMT val158met polymorphism: children with valine homozygote attention-deficit hyperactivity disorder have altered white matter connectivity in the right cingulum (cingulate gyrus)

MEHMET EMİN ERDAL | ÖZLEM İZCİ AY

Introduction: In this article, the COMT gene val158met polymorphism and attention-deficit hyperactivity disorder (ADHD)-related differences in diffusion-tensor-imaging-measured white matter (WM) structure in children with ADHD and controls were investigated. Patients and methods: A total of 71 children diagnosed with ADHD and 24 controls aged 8–15 years were recruited. Using diffusion tensor imaging, COMT polymorphism and ADHD-related WM alterations were investigated, and any interaction effect between the COMT polymorphism and ADHD was also examined. The effects of age, sex, and estimated total IQ were controlled by multivariate analysis of covariance (MANCOVA). Results: First, an interaction between the COMT val158met polymorphism and ADHD in the right (R) cingulum (cingulate gyrus) (CGC) was found. According to this, valine (val) homozygote ADHD-diagnosed children had significantly lower fractional anisotropy (FA) and higher radial diffusivity (RD) in the R-CGC than ADHD-diagnosed methionine (met) carriers, and val homozygote controls had higher FA and lower RD in the R-CGC than val homozygote ADHD patients. Second, met carriers had higher FA and axial diffusivity in the left (L)-uncinate fasciculus and lower RD in the L-posterior corona radiata and L-posterior thalamic radiat...

Microribonucleic acid dysregulations in children and adolescents with obsessive–compulsive disorder

MEHMET EMİN ERDAL | ÖZLEM İZCİ AY | MUSTAFA ERTAN AY | BAHAR TAŞDELEN

Aim: Obsessive–compulsive disorder (OCD) is a disorder characterized by the presence of obsessions and/or compulsions. Although disorder etiology and pathogenesis remains unknown, several theories about OCD development have been proposed, and many researchers believe that it is caused by both genetic and environmental factors. In the current study, our aim was to investigate miRNA levels in OCD. Methods: In the current study, we evaluated miR18a-5p, miR22-3p, miR24-3p, miR106b-5p, miR107, miR125b-5p, and miR155a-5p levels in child and adolescent OCD patients. The research sample consisted of a group of 23 OCD patients and 40 healthy volunteer controls. Results: There was no significant difference in age and sex between the two groups (P0.05).The levels of miR22-3p, miR24-3p, miR106b-5p, miR125b-5p, and miR155a-5p were significantly increased in the OCD subjects (P0.05). There were no statistically significant differences in miR18a-5p or miR107 levels between groups (P0.05). Conclusion: There could be a close relationship between levels of circulating miRNAs and OCD. If we could understand how the signaling pathways arranged by miRNAs impact on central nervous system development, function, and pathology, this understanding could improve our knowledge about OCD etiology and trea...

Association of VAMP-2 and Syntaxin 1A Genes with Adult Attention Deficit Hyperactivity Disorder

Ay, Özlem İzci | Erdal, Mehmet Emin

ObjectiveaaThe etiology of attention deficit hyperactivity disorder (ADHD) has not been entirely clarified yet. Structural and metabolic differences at the prefrontal striatal cerebellary system and the interaction of gene and environment are the main factors that thought to play roles in the etiology. Genetic investigations are performed especially about the dopamine pathways and receptors. In this study; it was aimed to investigate the association of the synaptobrevin-2 (VAMP-2) gene Ins/Del polymorphism and syntaxin 1A gene intron 7 polymorphism, which take place in encoding presynaptic protein, with adult ADHD. MethodsaaOne hundred thirty-nine patients, having ADHD aging between 18 and 60 years and 106 healthy people as controls were included into the study. DNA samples were extracted from whole blood and genetic analysis were performed. ResultsaaA significant difference was determined between ADHD and VAMP-2 Ins/Del polymorphism and syntaxin 1A intron 7 polymorphism according to the control group. These polymorphisms were found not to be associated with subtypes of ADHD. ConclusionaaIt is supposed that synaptic protein genes together with dopaminergic genes might have roles in the etiology of ADHD.

Possible Association of FAS and FASLG Polymorphisms with the Risk of Idiopathic Azoospermia in Southeast Turkey

Erdal, Mehmet Emin | Ay, Özlem İzci

To investigate the association of the genetic variants of FAS/FASLG cell death pathway genes in male infertility, we genotyped the FAS - 670A/G, - 1377G/A, and FASLG - 124A/G single-nucleotide polymorphisms (SNPs) by real-time polymerase chain reaction in 108 infertile men with idiopathic azoospermia and in 125 proven fertile controls. The distribution of genotypes and alleles for SNPs at FAS - 1377G/A and FASLG - 124A/G loci were determined not to be statistically different between the case and control groups. However, the genotype frequencies of SNPs, FAS - 670AA and FAS - 670AG, were found to be significantly different between the case and control groups. Whereas the FAS - 670AA genotype might be regarded as a higher predisposition for idiopathic azoospermia, FAS - 670AG could be interpreted to mean that this genotype provides protection against idiopathic azoospermia. The study of combined genotype and haplotype frequencies has found statistically significant differences between case and control subjects for some combinations. The AA-GG binary genotype for the FAS670 and FAS1377 loci couple, in particular, may have a high degree of predisposition to idiopathic azoospermia. Our results suggest that FAS - 670A/G SNP may be a genetic predisposing factor of idiopathic azoospermia...

The Possible Association of Polymorphisms in MTHFR, MTRR, and MTHFD1 Genes with Male Infertility

Erdal, Mehmet Emin | Ay, Özlem İzci

Objective: The aim of this study was to investigate the association of the methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), and methylenetetrahydrofolate dehydrogenase (MTHFD1) polymorphisms in idiopathic infertile men and fertile men. Materials and Methods: Case-control study comprising a total of 233 individuals including 108 idiopathic infertile men with nonobstructive azoospermia and 125 fertile men as control. MTHFR C677T, A1298C; MTRR A66G; and MTHFD1 G1958A polymorphisms were studied by Real-Time PCR System. The results were analyzed statistically and a P value

The Tumor Necrosis Factor-A -308 G/A Polymorphism and the Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Polymorphisms, in Asthmatic Patients and Healthy Subjects

Erdal, Mehmet Emin | Ay, Özlem İzci

Asthma is a chronic inflammatory disorder of the airways in which many cells and cellular elements play a role. Like other atopic diseases, asthma is a complex disorder caused by interactions between multiple genes of small to modest effect and equally important environmental factors. The aim of this study was to determine the TNF-α -308 G/A polymorphism and the TRAIL polymorphisms, and their influence on asthma in asthmatic patients and healthy subjects. The study population consists of 51 asthmatic patients (47 female and 4 male) and 72 healthy subjects (62 female and 10 male). The mean age of the asthmatic patients and healthy controls were 45.33±14.05, and 41.88±17.41 years, respectively. The asthmatic patients and healthy controls were similar with respect to their ages and sex characters. There was statistically a significant difference between the asthmatic patients and control groups in terms of TRAIL Arg141His, G422A (rs6557634) polymorphism (p=0.02). Statistically, there was not any significant difference between the asthmatic patients and control groups for TRAIL Thr209Arg, C626G (rs20575) TRAIL Glu228Ala, A683C (rs20576) and polymorphisms (p=0.57). Also, there was no significant difference between the asthmatic patients and control groups in terms of TNF-α-308 G/A pol...

The Tumor Necrosis Factor-A (TNF-A) Gene -308 G/A Polymorphism and the Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Gene Polymorphisms in Behcet'S Disease

Erdal, Mehmet Emin | Ay, Özlem İzci

Behcet's disease (BD) is a chronic, multisystemic inflammatory disease. The specific etiology of BD remains elusive, but the interaction between infectious-agent exposure and genetic factors may have a role. In this report, we aim to investigate the possible association between pathogenesis of the BD and TNF-α gene 308A/G (rs1800629) polymorphism and the TRAIL gene (Arg141His, G422A (rs6557634), Thr209Arg, C626G (rs20575) and Glu228Ala, A683C (rs20576)) polymorphisms in people from southeast of Turkey. The study population consisted of 55 BD patients and 80 healthy subjects. All samples were collected and studied between July 2009 and January 2010. Polymorphisms were detected by polymerase chain reaction restriction fragment length-polymorphism (PCR-RFLP) analysis. The patients and healthy control groups were similar with respect to their ages and sex characteristic. Statistically, there was not significant difference between the BD patients and healthy control groups in terms of TRAIL Arg141His, G422A (rs6557634) polymorphism, TRAIL Thr209Arg, C626G (rs20575), TRAIL Glu228Ala, A683C (rs20576) and TNF-α-308 G/A (rs1800629) polymorphisms. We could not detect statistically significant difference between the BD patients and healthy control groups according to TNF-α-308 G/A (rs180062...