Biochemical and Antimicrobial Properties of Alburnus tarichi Roe Protein Hydrolysate
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
The protein hydrolysates with antioxidant and antimicrobial potential have become a theme of great interest for pharmaceutical, nutraceuticals, as well as food processing and preservation industries. On the other hand, chemical composition of fish protein hydrolysates is significant in nutrition perspective of human health. Hence, in the present study proximate and amino acid compositions, antioxidant and antimicrobial activities of Alburnus tarichi roe protein hydrolysate was estimated. Protein, lipid, moisture, and ash content were found to be 87.24±0.1, 0.72±0.04, 8.79±0.3, and 7.01±0.09%, respectively. Glutamic acid, aspartic acid, lysine, leucine and alanine were found to be the most dominant amino acids, whereas, cysteine was found to be the lowest amino acid in roe protein hydrolys...
Roe protein hydrolysate of Alburnus tarichi induces apoptosis in breast cancer MCF-7 and MDA-MB-231 cells through a caspase-dependent pathway
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER
The protein hydrolysates of fishes have been reported to be a potential source of many health benefits components for pharmaceutical or nutritional applications. The aim of this study is to examine the possible antiproliferative function of roe protein hydrolysates of Alburnus tarichi using enzymatic hydrolysis against breast cancer cells and explore its detailed mechanisms. In addition, we evaluated the effects of protein hydrolysate on the proliferation and apoptosis of two human breast cancer cell lines (MCF-7 and MDA-MB-231). The cultured cells were treated with protein hydrolysate at concentrations of 0−5 μg/ml for 24 h and 48 h. Inhibition of cell proliferation, percentage of apoptotic cells, cell cycle distribution, morphological changes, DNA fragmentation, intracellular reactive o...