Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.
Effects of Chromium on the Hematocrit Levels and Erythrocyte Numbers of Cyprinus carpio
Cicik, Bedii | Çiftçi, Nuray | Ay, Özcan
Changes in hematocrit levels and erythrocyte numbers of Cyprinus carpio were determined after exposing the animals to 0.5, 1.0 and 2.0 ppm chromium over 7, 15 and 30 days. Microhematocrit and hemocytometric methods were used for determining hematocrit levels and erythrocyte numbers respectively. No mortality was observed during the experiments. Hematocrit levels and erythrocyt numbers increased under the effect of metal on day 15. Hematocrit levels and erythtrocyte numbers decreased by increasing exposure periods except at 0.5 ppm chromium.
Effect of natural adsorbent clinoptilolite on some hematological parameters of Cyprinus carpio
Çiftçi, Nuray | Cicik, Bedii
Effect of natural chelating agent clinoptilolite on hematocrit level, erythrocyte numbers, mean corpuscular volume (MCV), erythrocyte and erythrocyte nucleus area of Cyprinus carpio were determined after exposing the animals to 2, 4, 8, 16, 32 gL-1 clinoptilolite (0.40 µ) (3, 7, 21 and 30 days). Hematocrit levels were determined using microhematocrit methods and erythrocyte numbers, erythrocyte and its nucleus areas and MCV were determined using microscopic methods. Erythrocyte number and hematocrit levels decreased at 16 and 32 g L-1 of clinoptilolite with compared to control group in except 3 and 7 days of exposure (p
The protective role of ferulic acid against imidacloprid-induced oxidative stress in liver and brain of Cyprinus carpio
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER
This study evaluated the potential protective effect of ferulic acid (FA) on stress induced by imidacloprid (IM) in Cyprinus carpio (mean weight 60.10±2.22 g). Therefore, C. carpio was exposed to sublethal IM concentration (2.80 mg/L) for 10 days. During the experimental period, fish were fed a basal (control) diet and a basal diet supplemented with 5 g/kg of ferulic acid. The fish were divided into four groups (n = 6 in each group). The control group was exposed to tap water (the absence of imidacloprid) fed with control diet. The FA group was exposed to tap water (the absence of IM) and was fed with FA supplemented diet. The liver and brain tissues of both control and treated fish were dissected. Tissues samples were obtained from an individual fish and prepared for analysis. The variat...
Effects of dietary selenium of organic form against lead toxicity on the antioxidant system in Cyprinus carpio
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
In this study was evaluated potential protective effect of organic selenium (Se) on heavy metal stress induced by lead (Pb) in Cyprinus carpio. For this reason, C. carpio was exposed to sublethal concentration of Pb (1.5 mg/L Pb(NO3)2) for 14 days. The fish were fed a basal (control; measured 0.55 mg/kg Se) diet or a basal diet supplemented with 2.50 mg/kg (measured 2.92 mg/kg Se) organic Se (Sel-Plex®) during the experiment period. The variations in glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) activities, and levels of reduced glutathione (GSH) with malondialdehyde (MDA) in liver and brain tissues of C. carpio were investigated in experimental groups. GSH levels in liver and brain tissues were significantly decreased by exposure to Pb. GST activity was significantly in...