Electrochemical determination of phosphodiesterase-3 enzyme inhibitor drug Milrinone with nanodiamond modified paste electrode
Milrinone (MIL) is an essential cardiac inotrope drug for cardiovascular disease. In this study, a simple nanosensor based on a nanodiamond carbon paste electrode modified with flower-like ZnO particles was developed to determine MIL electrochemically rapidly and accurately. The flower-like ZnO nanoparticles were synthesized by a simple and cheap sonochemical method. The structure and morphology of the ZnO nanoparticles were investigated by Fourier transform infrared spectrometer and scanning electron microscopy. The electron transfer properties of bare carbon paste electrodes, nD@CPE, CPE/ZnO, and flower-like carbon paste electrodes modified with ZnO and decorated with nanodiamonds (nD@CPE/ZnO) were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). ...
Phosphodiesterase-3 Enzyme Inhibitor Drug Milrinone Interaction with DNA and HSA: Electrochemical, Spectroscopic and Molecular Docking
In this study, the interaction between the phosphodiesterase-3 enzyme inhibitor drug milrinone and biomolecules was investigated by electrochemical, fluorescence spectroscopy, and molecular docking studies for the first time. The interaction between milrinone and biomolecules was investigated according to the decrease in deoxyguanosine oxidation signals of milrinone and calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) by cyclic voltammetry and differential pulse voltammetry. In fluorescence spectroscopy studies, a competitive study was conducted on ct-dsDNA by adding a well-known fluorescent methylene blue and ct-dsDNA solution. The fluorescent results showed that milrinone had a higher affinity for ct-dsDNA binding compared to methylene blue. Interaction studies show that milr...