The copper accumulation in liver and gill tissues of Oreochromis niloticus, exposed to 2 ppm Cu
and 1g/L clinoptilolite singly and to the same concentrations of their mixture over 24, 48, 72 and
96 hours was studied. ICP-AES spectrophotometer techniques were applied in determining tissue
copper levels. Statistical evaluation of the experimental data was carried out by Student Newman
Keul’s procedure. No mortality was observed during the experiments. Copper accumulation was
lower in metal-clinoptilolite mixture group than metal singly group in gill tissue while no
accumulation in both experimental groups in liver tissue (P
Effects of sub-lethal concentrations of copper and lead
on hematocrit and mean cell volume (MCV) levels and on
erythrocyte numbers, erythrocyte and erythrocyte nucleus
areas were determined after exposing Oreochromis niloticus to 4 ppm Cu and 0.2 ppm Pb over 7, 15 and 30 days.
Micro-hematocrit methods were adopted in determining hematocrit levels and microscopic methods were used
to determine MCV, erythrocyte numbers, erythrocyte and
erythrocyte nucleus areas.
Hematocrit and MCV levels and erythrocyte and nucleus areas increased significantly (P