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169369

Genomewide association of male reproductive traits in Aksaray Malakli dogs

Tuncer, Pürhan Barbaros | Büyükleblebici Serhat

Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) geno type of the investigated characteristics and reproductive loci. A total of 46 male dogs aredividedintothreeagegroups(I≤3years,n = 19; II 4–6 years; n = 19, and III ≥7years,n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The A...

169396

The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen

Tuncer, Pürhan Barbaros | Büyükleblebici, Serhat

The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose . Insemination doses were processed that each 0.25-mL straw contained 15 106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics...

169489

Değişik sulandırıcılar ile dondurulan aygır spermalarının in vitro değerlendirilmesi üzerine çalışmalar

Tuncer, P. B.

The aim of this research was to investigate spermatolojical charecteristics of stallion semen frozen with lactose and tris extenders. This experiment was done in the Anadolu State Farm and five Pure Arabian Stallions were used. Semen taken from five Arabian Stallions were extended in a way that to ensure 100x106 /cm3 motile spermatozoa by lactose and tris extenders and frozen in the macrotube in liquid nitrogen vapour. In the semen after thawing of 500C-40 seconds the average values of semen; sperm motility (%),total percentage of abnormal sperm (%), percentage of abnormal acrosome (%) and thermo-rezistance test motility (%) in 1., 2. and 3. hours were recorded; %47.44; %31.73; %22.64; %22.74; %9.13 and %2.58 respectively for frozen semen by lactose. In the semen after thawing of 500C-...

169506

Evaluationofpost-thawqualityofBrown-SwissandHolsteinbullsemendilutedwith different extenders

Tuncer,, Pürhan Barbaros

Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p

169370

The Effect of Green Tea Extract Supplementation in Bull Semen Cryopreservation

TUNCER, Pürhan Barbaros

This study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters in frozen thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containing different amounts of CT (5, 10, 25 and 50 μg/mL) and no-additive (control). All samples were equilibrated at 4°C for 4 h and were frozen using a digital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. Spermatozoa DNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipid peroxidation status was evaluated colorimetrically. CT supplementation did not impr...

169495

Holştayn boğa spermalarının +40 C’de saklanmasında farklı sperma sulandırıcılarının motiliteye etkisi.

Tuncer, P. B.

This study was conducted to compare Laiciphose W 488 and AndroMed extenders for maintaining sperm motility at 40C in bulls. In the study 40 ejaculates collected from 4 Holstein bulls were used. Ejaculates were divided into two groups and were diluted with Laiciphos W 488 and AndroMed. Diluted semen was cooled to +40C in 45 minutes. After that they were transferred into the refrigerator. Sperm motility was estimated daily up to day 10 post dilution. Immediately after dilution sperm motility rates were 83.37±0.37 % and 83.12±0.38 % for Laiciphos W 488 and AndroMed extenders, respectively. Sperm motility for the samples diluted with AndroMed on day 3 was above 50 % in all bulls studied. However, motility rates in sperm samples diluted with Laiciphos W 488 were higher than 50% in 2 bulls onl...

169978

Boğa Spermalarının İki Farklı Sulandırıcı ile Dondurulması ve İn Vitro Değerlendirilmesi Freezing of Bull Semen with Two Different Extenders and In Vitro Evaluation

TUNCER, Pürhan Barbaros

In this study effect of AndroMed (AS) and Laiciphose W 488 (LS) extenders on the sperm motility and abnormal spermatozoa rates was examined in Holstein bull semen after they were diluted equilibrated and, thawed. In the study 40 ejaculates collected from 4 bulls were used. Averaga semen volume (8.47±0.69%), sperm concentration (x106/ml) 1030.55±7.11, and abnormal spermatozoa (3.47±0.18%) were found. The motility rates of fresh semen and after equilibration that diluted with LS and AS were 83.37±0.37%, 73.50±0.80% and 83.12±0.38%, 72.25±0.83% respectively. After thawing motility rates of sperm diluted with LS and AS extenders were 54.63±1.47%, 52.75±1.16% and, abnormal spermatozoa percentages of the sperm were 7.75±0.34% and 8.25±0.30%. According to this study, neither of these extenders a...

169380

Evaluation of some spermatological characteristics in Gerze cocks

TUNCER, Pürhan Barbaros

The aim of this research was to prove the principal spermatological characteristics in Gerze cocks in Turkey. In this experiment, 30 Gerze cocks were used. Semen was collected from cocks by abdominal massage method, two times a week during the 9 weeks period. The spermatological charecteristics of samples are as following: Ejaculate volume average 0.37±0.006 ml, spermatozoa motility 74.28±0.73%, spermatozoa concentration 2.42±0.02x109/ml, percentage of abnormal spermatozoa 6.32±0.10%, percantage of dead spermatozoa 19.74±0.73%, pH averaged 7.71±0.01 in Gerze cocks. Acrosome, head, middle piece and tail deformations of 540 ejaculates were recorded as average 0.39±0.03%, 1.06±0.03%, 2.32±0.05% and 2.53±0.04%, respectively. This is the first study which was proved some spermatological chara...

169388

Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen

Tuncer, Pürhan Barbaros

This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora goat semen after the freeze–thawing process. Ejaculates collected from four Angora goats were evaluated and pooled at 37 C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant (P < 0.05) increases in sper...

169395

Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage

Tuncer, Pürhan Barbaros

This study was conducted to determine the effects of methionine, inositol and carnitine on sperm (motility, abnormality, DNA integrity and in vivo fertility) and oxidative stress parameters (lipid peroxidation, total glutathione and antioxidant potential levels) of bovine semen after the freeze–thawing process. Nine ejaculates, collected with the aid of an artificial vagina twice a week from each Simmental bovine, were included in the study. Each ejaculate, splitted into seven equal groups and diluted in Tris-based extender containing methionine (2.5 and 7.5 mM), carnitine (2.5 and 7.5 mM), inositol (2.5 and 7.5 mM) and no additive (control), was cooled to 5 C and then frozen in 0.25 ml straws. Frozen straws were then thawed individually at 37 C for 20 s in a water bath for the evaluation...

169483

The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities

Tuncer, P. B.

The aim of the present study was to determine the effects of different doses of raffinose and methionine on pot thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck sperm following cryopreservation.

169398

Effect of glutamine and sugars after bull spermatozoa cryopreservation

Tuncer, Pürhan Barbaros | Büyükleblebici,Serhat

The objective of this study was to evaluate the effects of the addition of different sugars (raffinose, sucrose, and trehalose) on bull spermatozoa cryopreserved in a commercial extender (Optidyl) supplemented with glutamine on semen parameters, fertilizing ability and superoxide dismutase (SOD) activity. Nine ejaculates for each bull were used in the study. Semen was frozen in five different extenders: raffinose 25 mM plus glutamine 3 mM (RGO), sucrose 25 mM plus glutamine 3 mM (SGO), trehalose 25 mM plus glutamine 3 mM(TGO), glutamine 3 mM (GO) and control (O). Insemination doses were processed so that each 0.25 mL straw contained 15 x 106sperm. Groups of GO and RGO resulted in the higher rates of subjective (54.0 1.7% and 64.0 1.1%; P 0.01) and CASA motilities (53.0 2.7% and 61.0 4.4%; ...

169387

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Tuncer, Pürhan Barbaros

Cryopreservation is associated with the production of reactive oxygen substances (ROS), which lead to lipid peroxidation of sperm membranes, resulting in a loss of sperm motility, viability and fertility. The aim of this study was to determine effects of the antioxidants of oxidized glutathione (GSSG), reduced glutathione (GSH) and bovine serum albumin (BSA) on standard semen indicators (motility, acrosome and total abnormalities, HOST), endogenous antioxidant enzyme activities and fertilizing ability of frozen-thawed bull semen. Eighteen ejaculates from each of 3 Holstein bulls were collected using an artificial vagina and 9 replicates of the ejaculates were diluted with a Bioxcell®-based extender supplemented with antioxidants, including BSA (5 mg/ml), GSH (2 mM), GGSG (2 mM), and an ext...

169376

Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen

Tuncer,, Pürhan Barbaros

This study aimed to reveal the effects of juniper (Juniperus communis) berry oil (JB) when added to the Tris extender, on bull (Holstein) sperm motility characteristics, abnormal sperm ratio, DNA fragmentation and some oxidative stress markers. The pooled ejaculate was divided into five equal parts as control (C) and Tris diluent containing 25, 50, 100, 200 μg/ml JB. Diluted semen was incubated at 4 °C for 4 h and then frozen in liquid nitrogen (−196 °C). After freezing-thawing, motility characteristics were determined using a sperm analyzer system. The results showed that the 25 μg/ml dose of JB had an improving effect on overall sperm motility (P < 0.05). The total abnormal spermatozoa ratio was not significantly different between the groups (P > 0.05). The JB25 and JB50 groups had highe...

169379

Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze–thawing process

Tuncer, Pürhan Barbaros

The anti-oxidant system of reduced glutathione (GSH), glutathione peroxidase (GSH-PX), catalase (CAT), and superoxide dismutase (SOD) has been described as a defense functioning mechanism against lipid peroxidation (LPO) in semen, and is important in maintaining sperm motility and viability. This anti-oxidant capacity of sperm cells may be insufficient in preventing LPOduring the freeze–thawing process. The aim of this study was thus to determine the influence of varying doses of anti-oxidant additives on standard semen parameters, lipid peroxidation and anti-oxidant activities after the freeze–thawing of goat semen. Ejaculate samples (artificial vagina) obtained from 4 mature Angora goats were evaluated and pooled at 37◦C. The semen samples diluted with a Tris-based extender, contai...

169358

Effects of Various Antioxidants on Cryopreserved Bull Sperm Quality

TUNCER,, Pürhan Barbaros | Büyükleblebici, Serhat | Büyükleblebici, Olga

The objective of this study was to assess the effects of antioxidant supplement (A), fetuin (F), aminoacid (AS) and cysteine (CY) on the sperm parameters, plasma membrane integrity, chromatin damage and antioxidant activities after freeze-thawing. Ejaculates were split into five aliquots and extended to a final concentration of 15x106 spermatozoa/ml with the Tris base extender containing 0.5 ml A, 2 mg/ml F, 13% AS, 5 mM CY and no additive (C). The extended samples were equlibrated slowly to 4°C during 4 h and then frozen using a digital freezing machine.. Frozen straws were thawed individually in water bath at 37°C for 30 s to analyse progressive motility and sperm motion characteristics as well as membrane integrity. Biochemical assays were performed in a spectrophotometer using commer...

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