Cytotoxic effect of silica nanoparticles on human retinal pigment epithelial cells
Çömelekoğlu, Ülkü | Kibar, Deniz | Yıldırımcan, Saadet | Yılmaz, Şakir Necat
In recent years, the use of nanotechnology-based methods has become widespread in the treatment of ocular diseases. Silica nanoparticles (SiO2 NPs) are most common used NPs in medical field due to their physicochemical properties. SiO2 NPs can easily cross biological membranes and interact with basic biological structures, causing structural and functional changes in cells. In this study, it was aimed to investigate the dose dependent effect of SiO2 NPs on retinal pigment epithelium (RPE) in vitro using electrobiophysical, biochemical and histological methods. A commercially purchased human RPE (hARPE-19) cell line was used in this study. Cells were divided into four groups as control, 50 mg/mL SiO2, 100 mg/mL SiO2 and 150 mg/mL SiO2 groups. Cell index, apoptotic activity, cell cycle and ...
Determination of the effect of silica nanoparticles on TRP currents in retinal pigment epithelial cells by entropy measurement
Söğüt, Fatma | Kibar, Deniz | Yılmaz, Şakir Necat | Çömelekoğlu, Ülkü
Ion channels in cell membranes are gated, water-filled pores that allow passive transport of ions across the membrane along their electrochemical gradient. Recent studies have shown that nanoparticles can interact with ion channels and change their currents kinetics properties of the channel. In this study, we used the transient receptor potential (TRP) channel currents in retinal pigment epithelial (RPE) cells recorded by whole-cell patch clamp technique to observe the silica nanoparticle-ion channel interaction. For whole cell recordings, we clamped membrane potential to −40 mV and used a ramp of 1000 ms duration for stimulation. The ramp was increased from −140 mV to +60 mV. We used windowed scalogram entropy and compared the results with windowed scale index method. Our results indicat...