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Atesşahin Ahmet, Bucak Mustafa Numan, Kızıl Meltem1
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Baspınar Nuri, Çoyan Kenan, Bucak Mustafa Numan1
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Bucak Mustafa Numan, Sarıözkan Serpil, Baspınar Nuri, Taspınar Mehmet, Çoyan Kenan, Bilgili Ali, Akalın Pınar Peker, Aydos Sena, Ilgaz Seda , Sunguroğlu Asuman, Öztuna Derya1
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Bucak Mustafa Numan, Sarıözkan Serpil, Sakin Fatih, Ateşşahin Ahmet, Kulaksız Recai, Çevik Mesut1
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Bucak Mustafa Numan, Sarıözkan Serpil, Sakin Fatih, Yeni Deniz, Ciğerci İbrahim Hakki, Atessahin , Avdate Fatihk, Gündoğan Mustafa1
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Bucak Mustafa Numan, Sarıözkan Serpil, Ulutas Pınar Alkım1
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Bucak Mustafa Numan, Sarıözkan Serpil, Ulutaş Pınar Alkım , Akçadağ Halil İIbrahim1
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Bucak Mustafa Numan, Sarıözkan Serpil, Ulutaş Pınar Alkım , Çoyan Kenan, Başpınar Nuri, Özkalp Birol1
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Bucak Mustafa Numan, Sarıözkan Serpil, Yeni Deniz, Eken Ayse, Akalın Pınar Peker, Kinet Hüseyin, Avdatek Fatih, Fidan A. Fatih, Gündoğan Mustafa1
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Sariözkan Serpil, Bucak Mustafa Numan, Ulutaş Pınar Alkım1
Genomewide association of male reproductive traits in Aksaray Malakli dogs
Tuncer, Pürhan Barbaros | Büyükleblebici Serhat
Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) geno type of the investigated characteristics and reproductive loci. A total of 46 male dogs aredividedintothreeagegroups(I≤3years,n = 19; II 4–6 years; n = 19, and III ≥7years,n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The A...
The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen
Tuncer, Pürhan Barbaros | Büyükleblebici, Serhat
The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose . Insemination doses were processed that each 0.25-mL straw contained 15 106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics...
Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen
Tuncer, Pürhan Barbaros
This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora goat semen after the freeze–thawing process. Ejaculates collected from four Angora goats were evaluated and pooled at 37 C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant (P < 0.05) increases in sper...
Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage
Tuncer, Pürhan Barbaros
This study was conducted to determine the effects of methionine, inositol and carnitine on sperm (motility, abnormality, DNA integrity and in vivo fertility) and oxidative stress parameters (lipid peroxidation, total glutathione and antioxidant potential levels) of bovine semen after the freeze–thawing process. Nine ejaculates, collected with the aid of an artificial vagina twice a week from each Simmental bovine, were included in the study. Each ejaculate, splitted into seven equal groups and diluted in Tris-based extender containing methionine (2.5 and 7.5 mM), carnitine (2.5 and 7.5 mM), inositol (2.5 and 7.5 mM) and no additive (control), was cooled to 5 C and then frozen in 0.25 ml straws. Frozen straws were then thawed individually at 37 C for 20 s in a water bath for the evaluation...
Effect of glutamine and sugars after bull spermatozoa cryopreservation
Tuncer, Pürhan Barbaros | Büyükleblebici,Serhat
The objective of this study was to evaluate the effects of the addition of different sugars (raffinose, sucrose, and trehalose) on bull spermatozoa cryopreserved in a commercial extender (Optidyl) supplemented with glutamine on semen parameters, fertilizing ability and superoxide dismutase (SOD) activity. Nine ejaculates for each bull were used in the study. Semen was frozen in five different extenders: raffinose 25 mM plus glutamine 3 mM (RGO), sucrose 25 mM plus glutamine 3 mM (SGO), trehalose 25 mM plus glutamine 3 mM(TGO), glutamine 3 mM (GO) and control (O). Insemination doses were processed so that each 0.25 mL straw contained 15 x 106sperm. Groups of GO and RGO resulted in the higher rates of subjective (54.0 1.7% and 64.0 1.1%; P 0.01) and CASA motilities (53.0 2.7% and 61.0 4.4%; ...
Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen
Tuncer, Pürhan Barbaros
Cryopreservation is associated with the production of reactive oxygen substances (ROS), which lead to lipid peroxidation of sperm membranes, resulting in a loss of sperm motility, viability and fertility. The aim of this study was to determine effects of the antioxidants of oxidized glutathione (GSSG), reduced glutathione (GSH) and bovine serum albumin (BSA) on standard semen indicators (motility, acrosome and total abnormalities, HOST), endogenous antioxidant enzyme activities and fertilizing ability of frozen-thawed bull semen. Eighteen ejaculates from each of 3 Holstein bulls were collected using an artificial vagina and 9 replicates of the ejaculates were diluted with a Bioxcell®-based extender supplemented with antioxidants, including BSA (5 mg/ml), GSH (2 mM), GGSG (2 mM), and an ext...
Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze–thawing process
Tuncer, Pürhan Barbaros
The anti-oxidant system of reduced glutathione (GSH), glutathione peroxidase (GSH-PX), catalase (CAT), and superoxide dismutase (SOD) has been described as a defense functioning mechanism against lipid peroxidation (LPO) in semen, and is important in maintaining sperm motility and viability. This anti-oxidant capacity of sperm cells may be insufficient in preventing LPOduring the freeze–thawing process. The aim of this study was thus to determine the influence of varying doses of anti-oxidant additives on standard semen parameters, lipid peroxidation and anti-oxidant activities after the freeze–thawing of goat semen. Ejaculate samples (artificial vagina) obtained from 4 mature Angora goats were evaluated and pooled at 37◦C. The semen samples diluted with a Tris-based extender, contai...
The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities
Tuncer, Pürhan Barbaros | Büyükleblebici Olga
The aim of the present study was to determine the effects of different doses of raffinose and methionine on post-thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck (Capra hircus ancryrensis) sperm following cryopreservation. Ejaculates collected from three Angora bucks were evaluated and pooled at 37 C. Semen samples, which were diluted with a Tris-based extender containing the additives raffinose (2.5, 5, 10 mM) and methionine (2.5, 5, 10 mM) and an extender containing no antioxidants (control), were cooled to 5 C and frozen in 0.25 ml French straws. Frozen straws were thawed individually at 37 C for 20 s in a water bath for evaluation. The freezing extender supplemented with 2.5 and 5 mM methionine led to higher percentages of CASA motility (63.6 ± ...
Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation
Tuncer, Pürhan Barbaros
The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora goat semen. Ejaculates collected from three Angora goat bucks, were evaluated and pooled at 37◦C. The semen samples which were diluted with a Tris-based extender containing addi tives including glutamine (2.5; 5mM) and hyaluronan (500; 1000 l/ml), and an extender containingnoantioxidants(control)werecooledto5◦Candfrozenin0.25mlFrenchstraws and stored in liquid nitrogen. Frozen straws were thawed individually (37◦C) for 20s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5mMglutamine led to...
Comparison of the effects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities
Tuncer, Pürhan Barbaros
Ram semen contains sufficient quantities of superoxide dismutase (SOD) and much lower concentrations of glutathione peroxidase (GSH-PX) and catalase (CAT) to prevent oxidative damage. The anti-oxidant capacity of the sperm cell is limited, due to a small cytoplasmic component,which contains these anti-oxidants to scavenge the oxidants. However ,the concentration of these anti-oxidants may decrease considerably by the dilution of the semen. The aim of the present work was to study the effect of two anti-oxidants, namely ,glutamine and an amino acid solution (BME) in a Tris-based extender on ram sperm parameters, lipid peroxidation and anti-oxidant capacity after the cryopreservation/thawing process. Ejaculates collected from 4 Akkaraman rams were evaluated and pooled at 37◦C. Semen samples ...
Effects of dithioerythritol on ram semen after the freeze–thawing process
Tuncer, Pürhan Barbaros
The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extenderon the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2 mM dithiothreitol and no antioxidant (control), were cooled to 5 C and frozen in 0.25 ml French straws. Frozen straws were then thawed individually at 37 C for 20 s in a water bath for evaluation. The addition of dithioerythritol at 0.5 and 2 mM doses led to higher percentages of subjective motility (62.9 ± 4.2% and 63.6 ± 1.8%) compared to control (52.0 ± 4.9%, P < 0.05). As regards CASA motility, dithio erythrito...
The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities
Tuncer, Pürhan Barbaros
Theaimofthisstudywastodeterminetheeffectsoftheantioxidantscurcumin,inositoland carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora goat semen. Ejaculates were collected via artificial vagina from three Angora goats and microscopically evaluated and ooledat37◦C. The pooled semen samples were diluted in aT ris-based extender, including curcumin (2.5, 5 or 10mM), inositol (2.5, 5 or 10mM), carnitine (2.5, 5 or 10mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2–0.3◦C/min) cooled to 5◦C and then cryopreserved in 0.25mL French straws. Frozen straws were thawed individually at 37◦C for 20s in a water bath, for microscopic sperm evaluation. The freezing extender supple...
Use of Infrared Thermography during Ejaculation Process and Its Link with Semen Quality and Freezability in Dogs
Tuncer, Pürhan Barbaros | Büyükleblebici Serhat
Simple Summary: Scientific attention to infrared technology has grown over the last decade. Remote and non-invasive monitoring techniques are of great importance in discovering ejaculation response and future trends because of their role in vascular flux regulation. However, detailed information about its use in andrology has yet to be fully explained. Therefore, we aimed to reveal information about the amount of sperm to be obtained by observing stress levels with non-invasive eye temperature measurement, and the relationship between various reproductive temperature patterns and parameters of the animal’s various physiological conditions such as age, body condition, total ejaculation time and testicular volume.
The influence of cysteine and taurine on microscopic–oxidative stress parameters and fertilizing ability of bull semen following cryopreservation
Tuncer, Pürhan Barbaros
Oxidative stress significantly damages sperm functions such as motility, functional integrity, endogenous antioxidant enzyme activities and fertility due to lipid peroxidation induced by reactive oxygen species (ROS). The aim of this study was to determine the effects of antioxidants such as taurine and cysteine in Bioxcell extender on standard semen parameters, fertilizing ability, lipid peroxidation (LPO) and antioxidant activities comprising reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) after the cryopreservation/thawing of bull semen. Nine ejaculates for each bull were included in the study. Three groups, namely taurine (2 mM), cysteine (2 mM), and control, were designed to analyze the antioxidants in Bioxcell . Insemination...