Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.
The protective role of ascorbic acid (vitamin C) against chlorpyrifos-induced oxidative stress in Oreochromis niloticus
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN SERAP
Ability of ascorbic acid (vitamin C) to attenuate oxidative damage was evaluated in liver and brain tissues of Oreochromis niloticus (O. niloticus) experimentally exposed to sublethal concentrations of chlorpyrifos (CPF). O. niloticus was exposed to sublethal concentrations of CPF at 12 μg/L (CPF1) and 24 μg/L (CPF2) for 96 h. The fish of vitamin C (Vit C) and CPF2 + Vit C groups were fed with Vit C supplemented diet (200 mg Vit C/100 g feed). A significant increase in thiobarbituric acid-reactive substances (TBARS) level (P < 0.05) was observed in brain of CPF-exposed fish although liver TBARS level was not changed compared to control group. This result showed that lipid peroxidation (LPO) was elevated in brain of fish exposed to CPF. Glutathione peroxidase (GSH-Px) activity in liver and ...
Effect of Mannan Oligosaccharide on Growth Body Composition and Antioxidant Enzyme Activity of Tilapia Oreochromis niloticus
HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | YALIN, SERAP
The effects of three inclusion levels of mannan oligosaccharides (MOS) derived from the outer cell wall of a select strain of Saccharomyces cerevisiae (Bio-Mos, Alltech Inc, USA) on growth, feed utilization, body composition, and antioxidant enzyme activity of tilapia (Oreochromis niloticus) were determined. Specimens (12 g) were fed a commercial diet supplemented with 0‰, 2.5‰, 3.5‰, or 4.5‰ dietary MOS for 60 days. Food conversion rates (FCR), specific growth rates (SGR), and the biochemical composition of muscle tissue were determined. Growth was greatest, protein was highest, and lipid was lowest in fish fed the 2.5‰ MOS diet. The FCR was significantly better in all MOS-treated groups than in the unsupplemented control. At the end of the study, antioxidant enzyme activity of superoxid...
Effects of Organic Selenium on Growth, Muscle Composition, and Antioxidant System in Rainbow Trout
HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | YALIN, SERAP
This experiment was conducted to understand the effects of organic selenium on rainbow trout, Oncorhynchus mykiss. Fish (33.47±0.15 g; n = 216) were randomly assigned to four treatment groups consisting of three replicates of 18 fish each in 100 x 100 x 125 cm cages. The fish were fed a basal diet supplemented with 2, 3, or 4 mg/kg organic Se (Sel-Plex®) supplementation for 8 weeks. Muscle of fish fed the unsupplemented control diet had a lower (p
Influence of Sublethal Chlorpyrifos Exposure on Oxıdative Stress and Acetylcholinesterase Activity in Common Carp (Cyprinus carpio)
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
The commonly used pesticides in agriculture cause enzyme inactivation and DNA damage via reacting with macromolecules and may also initiate peroxidation of poly-unsaturated fatty acids (PUFA) by generating of the reactive oxygen species (ROS), as a consequence they can lead to the oxidative stress. The objective of this study was to determine the oxidative and neurotoxic potential of sublethal chlorpyrifos in the brain tissue of Cyprinus carpio (C. carpio) for a period of 96 and 240 hours, respectively. Therefore, the levels of malondialdehyde (MDA), glutathione (GSH), advanced oxidation protein products (AOPP), protein carbonyl, and 8-hydroxydeoxyguanosine (8-OHdG) levels and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE...
Effects of Dietary Nucleotide Yeast on Immune Responses and Antioxidant Enzyme Activities of Rainbow Trout Juveniles (Oncorhynchus mykiss)
HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | ENGİN, KENAN | YALIN, SERAP
This study aimed at demonstrating the effects of dietary supplementation of nucleotide yeast base protein (Nu-Pro®) (NP) on the antioxidant enzyme activities and immune response in liver and blood tissues of rainbow trout (Oncorhynchus mykiss). Fish with an average initial weight of 27.75±0.26 g were randomly assigned to four groups with three replicates. Throughout the 60 day grow-out period the control group was fed a fish meal based basal diet, and three other groups were fed diets in which 20 (NP 20), 40 (NP 40) and 60 % (NP 60) fish meal was substituted with nucleotide (Nu-Pro® (NP) yeast). There were no significant changes of superoxide dismutase (SOD) and catalase (CAT) activities in liver among the experimental groups. A significant decrease (P
Effects of dietary selenium of organic form against lead toxicity on the antioxidant system in Cyprinus carpio
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
In this study was evaluated potential protective effect of organic selenium (Se) on heavy metal stress induced by lead (Pb) in Cyprinus carpio. For this reason, C. carpio was exposed to sublethal concentration of Pb (1.5 mg/L Pb(NO3)2) for 14 days. The fish were fed a basal (control; measured 0.55 mg/kg Se) diet or a basal diet supplemented with 2.50 mg/kg (measured 2.92 mg/kg Se) organic Se (Sel-Plex®) during the experiment period. The variations in glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) activities, and levels of reduced glutathione (GSH) with malondialdehyde (MDA) in liver and brain tissues of C. carpio were investigated in experimental groups. GSH levels in liver and brain tissues were significantly decreased by exposure to Pb. GST activity was significantly in...
The effects of fish meal replacement by yeast based nucleotides on growth body composition and digestive enzyme activity in rainbow trout juveniles Oncorhynchus mykiss
HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | ENGİN, KENAN | YALIN, SERAP | ŞAHİN, NEFİSE ÖZLEN
This 60 day study investigated the effects of organically certified nucleotide yeast-derived protein source (Nu-Pro®) on growth, feed efficiency, fillet proximate composition and digestive enzymes in rainbow trout (Onchorhyncus mykiss). Diets were isonitrogenous and isocaloric. Three experimental diets wherein 20%, 40% and 60% of the fish meal content was replaced by Nu-Pro®, were compared to the control diet in which the crude protein content was anchovy fish meal and corn gluten meal. The rainbow trout (initial weight 27 g/fish) were fed twice daily in 200-l Aqaria. Live weight gain increase ranged from 125-195% in fish fed the experimental diets. Results indicated that up to 40% fish meal protein can be replaced by Nu-Pro® without compromising growth rates, feed efficiency or the fillet...
Induction of micronuclei and other nuclear abnormalities in peripheral erythrocytes of Nile tilapia, Oreochromis niloticus, following exposure to sublethal cadmium doses
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER
The genotoxic effects of sublethal doses of cadmium in peripheral erythrocytes of Oreochromis niloticus was investigated using micronucleus (MN) and nuclear abnormalities (NAs) tests. For this reason, the fish were exposed to medium changes of 0.5 and 1.0 mg/L doses of cadmium (Cd) during an average treatment period of 10 days. On the 2nd, 4th, 6th, and 10th days of the test period, the erythrocytes, including the micronuclei (MN) and other nuclear abnormalities such as lobbed nuclei (LB), blebbed nuclei (BL), and notched nuclei (NT), were counted and pointed out. As a positive control, a 4 mg/L dose of cyclophosphamide was used. Exposure to doses of cadmium caused significantly increased (P < 0.05) micronuclei in peripheral erythrocytes of fish. It was also observed that the application o...