Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.
Protective Effect of Humic Substances Against Oxidative Stress Caused by Lead in Liver of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758)
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
In this study, the protective effect of humic substance in the fish feed was investigated against the effects of subletal lead (Pb) concentration on the antioxidant enzyme system in Oreochromis niloticus. For this purpose, the fish which were affected by 1.5 mg/L lead nitrate Pb(NO3)2 medium concentration were fed with feed containing 0.2% of humic substance. Liver samples were taken at 4th and 10th days in this study, which included control group, humic substance (HM), Pb, and Pb + HM groups. The enzyme activity of liver tissue catalase (CAT) and superoxide dismutase (SOD) of O. niloticus and the level of malondialdehyde (MDA), a product of lipid peroxidation, increased significantly at 4th and 10th days in the group with subletal lead concentration compared with the control group. Compar...
Investigation of Glutathion S-Transferase, Adenosine deaminase, Paraoxonase Activities in Liver of Oncorhynchus mykiss Fed with Nucleotide-Yeast Supplemented Diet
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER
In this study, it was evaluated Glutathione S transferase (GST), Adenosine deaminase (ADA), and paraoxonase (PON) activities in liver tissue of Oncorhynchus mykiss fed with nucleotide yeast base protein supplemented diet. Throughout the 60-day period the control group was fed a fish meal based basal diet, and three other groups were fed diets in which 20% (NP 20), 40% (NP 40) and 60% (NP 60) fish meal was substituted with nucleotide (Nu-Pro® (NP) yeast). At the end of experiment, liver tissue GST, PON and ADA activity was increased significantly (P
The protective role of ferulic acid against imidacloprid-induced oxidative stress in liver and brain of Cyprinus carpio
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER
This study evaluated the potential protective effect of ferulic acid (FA) on stress induced by imidacloprid (IM) in Cyprinus carpio (mean weight 60.10±2.22 g). Therefore, C. carpio was exposed to sublethal IM concentration (2.80 mg/L) for 10 days. During the experimental period, fish were fed a basal (control) diet and a basal diet supplemented with 5 g/kg of ferulic acid. The fish were divided into four groups (n = 6 in each group). The control group was exposed to tap water (the absence of imidacloprid) fed with control diet. The FA group was exposed to tap water (the absence of IM) and was fed with FA supplemented diet. The liver and brain tissues of both control and treated fish were dissected. Tissues samples were obtained from an individual fish and prepared for analysis. The variat...
Influence of Sublethal Chlorpyrifos Exposure on Oxıdative Stress and Acetylcholinesterase Activity in Common Carp (Cyprinus carpio)
YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP
The commonly used pesticides in agriculture cause enzyme inactivation and DNA damage via reacting with macromolecules and may also initiate peroxidation of poly-unsaturated fatty acids (PUFA) by generating of the reactive oxygen species (ROS), as a consequence they can lead to the oxidative stress. The objective of this study was to determine the oxidative and neurotoxic potential of sublethal chlorpyrifos in the brain tissue of Cyprinus carpio (C. carpio) for a period of 96 and 240 hours, respectively. Therefore, the levels of malondialdehyde (MDA), glutathione (GSH), advanced oxidation protein products (AOPP), protein carbonyl, and 8-hydroxydeoxyguanosine (8-OHdG) levels and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE...