hsa‑miR‑17‑5p: A Possible Predictor of Ombitasvir/Paritaprevir/ Ritonavir+Dasabuvir±Ribavirin Therapy Efcacy in Hepatitis C Infection

Zehra Öksüz | Enver Üçbilek | Mehmet Sami Serin | Serkan Yaraş | Gülhan Örekici | Temel Orhan Sezgin

Although persistent sustained viral response rates are increased in hepatitis C infection following administration of direct acting antiviral (DAA) agents, the pre-use predictive parameters of these antivirals and the clinical progression in patients post-treatment remain unknown. To obtain data pertaining to the predictive parameters prior to the use of ombitavir/paritaprevir/ritonavir+dasabuvir and the clinical progression in patients following antiviral treatment. The expression profles of miR-223-3p, miR-17-5p, miR-24-3p, and TLR2 − 196 to − 174 del/ins polymorphisms from the blood/serum of 34 hepatitis C virus (HCV)-infected patients pre- and post-ombitavir/paritaprevir/ritonavir+dasabuvir treatment were determined by RT-qPCR. The expression levels of miR-17-5p (P0.05); neverthe...

An X-Ray Study for Structural Characterization of cis-Bis(methyl 2-(benzoylcarbamothioyl)-3-(4-chlorophenyl)-5-methyl-4,6-dioxo-1-phenyl-octahydropyrrolo[3,4-c]pyrrole-1-carboxylate)platinum(II)

Nural, Yahya | Gemili, Muge

2025-12-29

Synthesis of novel polysubstituted thiazole derivatives

Nural, Yahya | Gemili, Muge

2025-12-29

Stereoselective synthesis of 2-(aminomethyl)-1-aminocyclopropanecarboxylic acid derivatives and their application in peptide design

Nural, Yahya

2025-12-29

Efficient synthesis of functionalized 3-aryl-1,3-aminoalcohols

Nural, Yahya | Dondas, Haci Ali

2025-12-29

Trends in electrochemical nanosensors for the analysis of antioxidants

Antioxidants play a very critical role in many biological processes in which free radicals are present. The physiological part of these compounds prevents damage to cellular components from arising because of chemical reactions involving free radicals. Oxidation mechanisms appear continuously in living metabolism, and reactive oxygen species are taken from outside step-up oxidation events. Many attempts have been made rapid, economical, and user-friendly analytical approaches for detecting and determining antioxidant capacity in recent years. Electrochemical techniques offer an alternative and powerful strategy for determining antioxidant content as they allow simple and inexpensive detection. This review discusses information about antioxidants, their classification, mechanism of action, ...

Novel 1,4-naphthoquinone N-aroylthioureas: Syntheses, crystal structure, anion recognition properties, DFT studies and determination of acid dissociation constants

Gemili, Müge | Nural, Yahya

In this paper, five novel 1,4-naphthoquinone N-aroylthiourea derivatives were synthesized, by the reaction of 2,3-diaminonaphthalene-1,4-dione and aroylisothiocyanates, in excellent yield (88-96%) and characterized by using FT-IR, H-1/C-13 NMR and HRMS. In addition, the molecular structure of one of the 1,4-naphthoquinone N-aroylthiourea compounds was characterized by single crystal X-ray diffraction studies. The absorption spectra of the compounds were determined in DMSO and the dyes have absorption in visible region in the range of 464-477 nm. The chemosensing ability of each of the prepared dyes was assessed by titrating them towards the anions; F-, Cl-, Br-, I- AcO-, CN-, CIO4-, H2PO4-, HSO4-, and NO3-, using spectrophotometric and H-1 NMR titration techniques in DMSO, experimentally. ...

Synthesis, antimycobacterial activity, and acid dissociation constants of polyfunctionalized 3-[2-(pyrrolidin-1-yl)thiazole-5-carbonyl]-2H-chromen-2-one derivatives

Nural, Yahya

This study reports on synthesis and determination of antimycobacterial activity and acid dissociation constants of polyfunctionalized 3-[2-(pyrrolidin-l-yl)thiazole-5-carbonyl[-2H-chromen-2-one derivatives, containing thiazole, coumarin, and pyrrolidine octahydropyrrolo[3,4-c]pyrrole moieties. The products were synthesized by a cyclization reaction of 5,5-diphenylpyrrolidine N-aroylthioureas or methyl 5-substituted 4,6-dioxo-3,3-diphenyloctahydropyrrolo[3,4-c[pyrrole-lcarboxylate N-aroylthioureas and 3-(bromoacetyl)coumarin with good to excellent yield (81-97%). The compounds exhibited antimycobacterial activity against the M. tuberculosis H37Rv strain with minimum inhibitory concentration values in the range of 31.25-125 mu g/cm(3). Acid dissociation constants of the compounds were determ...

Synthesis, crystal structure, DFT studies, acid dissociation constant, and antimicrobial activity of methyl 2-(4-chlorophenyl)-7a-((4-chlorophenyl)carbamothioyl)-1-oxo-5,5-diphenyl-3-thioxo-hexahydro-1H-pyrrolo[1,2-e]imidazole-6-carboxylate

Nural, Yahya | Gemili, Muge | Ulger, Mahmut

A novel bicyclic thiohydantoin fused to pyrrolidine compound, methyl 2-(4-chlorophenyl)-7a-((4-chlorophenyl)carbamothioyl)-1-oxo-5,5-diphenyl-3-thioxo-hexahydro-1H-pyrrolo[1,2-e]imidazole-6-carboxylate, was synthesized by the cyclization reaction of dimethyl 5,5-diphenylpyrrolidine-2,4-dicarboxylate and 4-chlorophenyl isothiocyanate in the presence of 4-(dimethylamino)pyridine to form methyl 2-(4-chlorophenyl)-1-oxo-5,5-diphenyl-3-thioxo-hexahydro-1H-pyrrolo[1,2-e]imidazole-6-carboxylate with concomitant addition reaction of the 4-chlorophenyl isothiocyanate in 79% yield. The structural characterization was performed by NMR, FT-IR, MS and HRMS techniques, and the stereochemistry of the compound was determined by single crystal X-ray diffraction study. In addition, the molecular structure a...

Synthesis of novel immunomodulatory 1,4-disubstituted bis-1,2,3-triazoles by using click chemistry and their intracellular mechanism of action

Nural, Yahya | Acar, Irem | Yetkin, Derya | Efeoglu, Cagla | Ayaz, Furkan

In this study, six new 1,4-disubstituted bis-1,2,3-triazole compounds, N,N '-(1,2-phenylene)bis(2-(4-R-1H-1,2,3triazol-1-yl)acetamide), were synthesized with high yield (88-96 %) by using click chemistry and their molecular structures were characterized by using NMR, FT-IR, HRMS and elemental analysis techniques. Previous studies suggest anti-inflammatory and analgesic activities for different 1,2,3-triazole derivatives and in the light of those studies we aimed to examine these novel derivatives immunomodulatory activities on the mammalian macrophages. Pro-inflammatory cytokines (TNF, IL6, GMCSF and IL12p40) secretion levels were tested in the presence of bis-1,2,3-triazole compounds when the macrophages were activated with LPS. These new derivatives were able to suppress the product...

Does anti-IgE therapy prevent chronic allergic asthma-related bone deterioration in asthmatic mice?

Nural, Yahya

Current evidence on the association between allergic diseases and bone metabolism indicates asthma may be a potential risk factor for bone health. Using anti-IgE has been proven effective in allergic asthma treatment with a good safety profile; however, its effects on bone health are unknown. Thus, we aimed to investigate whether: (i) chronic allergic asthma (CAA) causes any meaningful changes in bone, and if any, (ii) anti-IgE therapy prevents any CAA-induced adverse alteration. A murine model was used to study CAA. Thirty-two BALB/c male-mice were assigned into four groups (eight-mice/group): Control, CAA (treated with saline), CAA + 100 mu g of anti-IgE (CAA + 100AIgE), and CAA + 200 mu g of anti-IgE (CAA + 200AIgE) groups. After immunization, saline or anti-IgE was performed intraperit...

Serum zinc and alkaline phosphatase values in pediatric bone marrow transplantation patients

Zinc (Zn) plays an important role in the maintenance of immune functions, including cellular/humoral immunity, and in the prevention of oxidative injury. Therefore, the maintenance of a normal Zn status may be important in bone marrow transplantation (BMT) patients. Serum Zn levels were determined in 35 children during the BMT period. In addition, as Zn-related factors, serum Cu levels and alkaline phosphatase (AP) activity were also measured. There was a significant decrease in Zn and AP values during the immediate post-transplant period (lowest at day +7) when compared to pre-BMT levels (p < .01). The patients who developed hypozincemia were more likely to be transplanted for a diagnosis of malignant disorder and were younger, and adverse events appeared to occur more frequently. This pr...

Novel highly functionalized 1,4-naphthoquinone 2-iminothiazole hybrids: Synthesis, photophysical properties, crystal structure, DFT studies, and anti(myco)bacterial/antifungal activity

Gemili, Muge | Nural, Yahya | Ülger, Mahmut | Erat, Selma

In this paper, a series of novel highly functionalized 1,4-naphthoquinone 2-iminothiazole hybrids were synthesized via a cyclization reaction of 2,3-diaminonaphthalene-1,4-dione N-aroylthioureas and alpha-bromoketones in good to excellent yields (74-94%). The stereochemistry of one of the 1,4-naphthoquinone 2-iminothiazole products was characterized by single crystal X-ray diffraction technique. Photophysical properties of the compounds in DMSO, HAc and THF were determined by using UV-vis spectroscopy. The ground state geometries and the absorption spectra of the compounds were also determined using Density Functional Theory (DFT) and time-dependent DFT (TD-DFT) at B3LYP/631 + g(d,p) level of theory. The computed results are consistent with experimental values. An antimicrobial screening w...

Exploring bisbenzimidazole-chalcone hybrid compounds: Dual-action as antibacterial agents targeting biofilm formation and E. coli DNA gyrase inhibition

ADAR ERCAN | ÖKSÜZ ZEHRA | DÖĞEN AYLİN | ALGÜL ÖZTEKİN

The emergence of antibiotic-resistant pathogens has reduced the efficacy of current antimicrobial therapies, emphasizing the need for new therapeutic agents. This study presents the design, synthesis, and evaluation of bisbenzimidazole-chalcone hybrid compounds as potential antimicrobial agents. These compounds were tested for their antimicrobial activity against eleven common pathogens, as well as their ability to inhibit biofilm formation and eradicate preformed biofilms in Escherichia coli. Compounds EA1, EA3, EA4, and EA5 demonstrated antibacterial activity against E. coli comparable to ampicillin (31.25 μg/mL) and outperformed the other tested compounds. Notably, EA4 and EA5 inhibited biofilm formation at sub-MIC concentrations and effectively eradicated preformed biofilms, as ...

Synthesis, biological properties, and acid dissociation constant of novel naphthoquinone–triazole hybrids.

Nural, Yahya | Ozdemir, Sadin | Demir, Bunyamin | Yalcin, Mustafa Serkan | Erat, Selma

A series of novel 1,4-naphthoquinone-triazole hybrids, N-(3-amino-1,4-dioxo-1,4-dihydronaphthalen-2-yl)-2-(4-R-1H-1,2,3-triazol-1-yl)acetamide, was synthesized by click chemistry in the presence of sodium ascorbate and copper(II) sulfate pentahydrate in 81-94% yield. Various biological properties of the synthesized compounds including DNA binding/cleavage, antioxidant, antibacterial and antifungal properties were evaluated. The DNA binding study was performed using dsDNA and G-quadruplex DNA. All of the compounds showed fluorescence increase in the presence of DNA, regardless of the structure. Up to 2.9 and 2.5 times fluorescence increase upon incubation with double stranded or G-quadruplex DNA was detected for 5f and 5g, respectively. The docking studies performed on dsDNA and G-quadruple...

Determination of zinc and lead in human hair by atomic absorption spectrometry after digestion with tetramethylammonium hydroxide and conventional methods

Dogan, Selda

Various digestion methods were compared for determination of zinc and lead in human hair by atomic absorption spectroscopy. For the digestion of hair samples, alkaline digestion in tetramethylammonium hydroxide was used in addition to conventional acidic digestion methods like dry ashing, wet ashing and microwave digestion in acid. The accuracy of the methods was tested by certified standard reference material, CRM BCR-397 human hair. According to the statistical evaluation of the results, there are no significant differences between the four digestion methods. Relative errors of the certified value of CRM BCR-397 were less than 10% except for the tetramethyl-ammonium hydroxide digestion method.