Bulunan: 7 Adet 0.001 sn
Araştırmacılar Gönül Aslan
Gönül AslanTIP FAKÜLTESİ TEMEL TIP BİLİMLERİ BÖLÜMÜ TIBBİ MİKROBİYOLOJİ ANABİLİM DALI
170531

Epidemiological and molecular characterization of Enterobius vermicularis cases in Mersin, Turkey: An analysis based on mitochondrial DNA cox1 gene sequences

Gülbudak Harun | Tezcan Ülger Seda | Bozok Taylan | Aslan Gönül

Enterobius vermicularis is among the most common intestinal helminths, especially in children. Mitochondrial cox1 gene analyses revealed three genotypes (A, B, C). We aimed to evaluate the epidemiological characteristics and cox1 genotypes of E. vermicularis cases diagnosed at Mersin University Hospital. Between January 2017 and December 2023, 1496 samples were examined using the cellophane tape method, and 75 positives were identified. Epidemiological data were retrospectively analyzed. DNA was isolated from 30 positive samples via the phenol–chloroform method, and nested PCR products of the cox1 gene were sequenced. Phylogenetic analyses were performed using GenBank sequences. In a sample of patients with gastrointestinal signs, the positivity rate for E. vermicularis was 5 % (75/1496)...

Makale2025One Health 3 | 0 Erişime Açık
167372

Alpha Human Papillomavirus Types and Genotype Distribution in Psoriasis Patients: A Cross-Sectional Regional Study in Turkey.

Leyla Ersoy | Seda Tezcan Ülger | Ayça Cordan Yazıcı | Gönül Aslan

Background: Human papillomaviruses (HPVs) are members of the P apillomaviridae family, characterized by circular, double- stranded DNA. T hey exhibit strict tropism, infecting either mucosa or skin. T he role of HPV in the etiology of psoriasis remains unclear. Objectives: T he aim of this study was to determine the presence of alpha human papillomavirus in the skin scrapings of patients with psoriasis and its genotype distributions. Methods: T he study employed a cross-sectional design and included 53 psoriasis patients and 47 healthy controls from December 2020 to May 2021. After DNA isolation from skin scrapings, the L1 gene region of HPV was amplified using MY09/11 and GP5 +/6 + consensus primer sequences. T he HPV genotypes were determined through direct DNA sequence analysis of polym...

Makale2025Jundishapur J Microbiol 17 | 0 Erişime Açık
170645

Evaluation of cutaneous leishmaniasis cases in Mersin, Türkiye: Impact of migration and the COVID-19 pandemic

Gülbudak Harun | Bozok Taylan | Tezcan Ülger Seda | İkizoğlu Güliz | Delialioğlu Nuran | Aslan Gönül

Introduction: Cutaneous leishmaniasis (CL) is a significant public health concern in Türkiye, especially in the Southeastern Anatolia and Mediterranean regions. This study evaluated the epidemiological characteristics of CL cases diagnosed at Mersin University Hospital, including the impact of migration and the COVID-19 pandemic. Methodology: The diagnosis of CL was confirmed by microscopic examination of lesion smears. In this study, data from 144 patients diagnosed with CL between January 2011 and December 2022 were evaluated retrospectively. Demographic characteristics of the patients, temporal distribution (by month and year), and lesion features (location, number, and duration) were included. Results: CL was confirmed in 32.9% of patients (144/438). Of the 144 patients, 82 (56.9...

167488

Phenotypic and genetic homogeneity of Yersinia ruckeri strains isolated from farmed rainbow trout, Oncorhynchus mykiss (Walbaum), in Mersin Province, Turkey

ÖZER, Selmin | DÖNMEZ, Erdem | KOYUNCU, Erkin | SERİN, Mehmet Sami | ASLAN, Gönül | TEZCAN, Seda | AYDIN, Esin | EMEKDAŞ, Gürol

Twenty-four Yersinia ruckeri strains isolated from farmed rainbow trout, Oncorhynchus mykiss (Walbaum), in Mersin Province (Turkey) were investigated for phenotypic and genetic homogeneity by conventional microbiological techniques, the ID32GN system and arbitrarily primed polymerase chain reaction (AP-PCR). All isolates displayed phenotypic and genetic homogeneity by these techniques.

169668

Investigation of efflux pump genes in isoniazid resistant Mycobacterium tuberculosis isolates

Kaya, Hamide | Ersoy, Leyla | Ülger, Mahmut | Bozok, Taylan | Aslan, Gönül

Background: Tuberculosis (TB) is one of the most important infectious diseases worldwide. Resistance to antituberculosis drugs develops because of genetic mutations that render drug-activating enzymes inactive, changes in cell wall permeability, and increased expression of efflux pump genes and also combination therapy with efflux pump inhibitors may be more effective in drug-resistant TB patients. Aims: To investigate the effect of verapamil (VR) on isonicotinic acid hydrazide (INH) resistance and the expression of 21 efflux pump genes in INH monoresistant MTBC clinical isolates. Study design: In vitro study. Methods: In our mycobacteriology laboratory, 10 INH monoresistant and 10 primary anti-TB drug-susceptible MTBC clinical isolates were selected. Drug susceptibilities for INH and V...

167490

Genetic diversity and antimicrobial susceptibility of motile aeromonads isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) farms

ÖZER, Selmin | BULDUKLU, Sevim | TEZCAN, Seda | DÖNMEZ, Erdem | AYDIN, Esin | ASLAN, Gönül | EMEKDAŞ, Gürol

A total of 22 motile Aeromonas strains were detected in 48 (18.53%) of 259 fish and 6 (10.71%) of 56 water samples obtained from seven commercial rainbow trout (Oncorhynchus mykiss, Walbaum) farms in the province of Mersin, Turkey. These strains were identified by conventional microbiological techniques and by using an ID32GN system. Of these isolates 20 (91.3%) were identified as Aeromonas hydrophila and 2 (8.7%) as Aeromonas sobria. While 8 of the A. hydrophila strains were isolated from water samples, 12 isolates were from fish samples. Whereas A. hydrophila strains were found in all farms, A. sobria was detected in only two farms. Genetic diversity by arbitrarily primed polymerase chain reaction (AP-PCR) and antimicrobial sensitivity tests were carried out on eight A. hydr...

167487

Genetic Heterogeneity and Antibiotic Susceptibility of Vibrio alginolyticus Strains Isolated from Horse-Mackerel (Trachurus trachurus L., 1758)

ÖZER, Selmin | ASLAN, Gönül | TEZCAN, Seda | EMEKDAŞ, Gürol | SERİN, Mehmet Sami

Abstract: This study was performed to investigate the presence of halophylic Vibrio spp. in horse-mackerel (Trachurus trachurus L., 1758). In total, 80 fish samples were collected from fish markets and supermarkets between 04.04.2006 and 16.05.2006 in the city of Mersin. All fish samples were microbiologically tested for halophylic Vibrio spp. Nine (11.25%) Vibrio spp. strains were isolated and identified as V. alginolyticus. Molecular epidemiological discrimination and antimicrobial susceptibility patterns of V. alginolyticus strains were also analysed by arbitrarily primed polymerase chain reaction (AP-PCR) and Kirby-Bauer disc diffusion, respectively. Genotypic heterogeneity of these 9 isolates discriminated by AP-PCR determined that while only 3 isolates were from the same genotypic s...