Investigation of efflux pump genes in isoniazid resistant Mycobacterium tuberculosis isolates
Kaya, Hamide | Ersoy, Leyla | Ülger, Mahmut | Bozok, Taylan | Aslan, Gönül
Background: Tuberculosis (TB) is one of the most important infectious diseases worldwide. Resistance to antituberculosis drugs develops because of genetic mutations that render drug-activating enzymes inactive, changes in cell wall permeability, and increased expression of efflux pump genes and also combination therapy with efflux pump inhibitors may be more effective in drug-resistant TB patients. Aims: To investigate the effect of verapamil (VR) on isonicotinic acid hydrazide (INH) resistance and the expression of 21 efflux pump genes in INH monoresistant MTBC clinical isolates. Study design: In vitro study. Methods: In our mycobacteriology laboratory, 10 INH monoresistant and 10 primary anti-TB drug-susceptible MTBC clinical isolates were selected. Drug susceptibilities for INH and V...
Alpha Human Papillomavirus Types and Genotype Distribution in Psoriasis Patients: A Cross-Sectional Regional Study in Turkey.
Leyla Ersoy | Seda Tezcan Ülger | Ayça Cordan Yazıcı | Gönül Aslan
Background: Human papillomaviruses (HPVs) are members of the P apillomaviridae family, characterized by circular, double- stranded DNA. T hey exhibit strict tropism, infecting either mucosa or skin. T he role of HPV in the etiology of psoriasis remains unclear. Objectives: T he aim of this study was to determine the presence of alpha human papillomavirus in the skin scrapings of patients with psoriasis and its genotype distributions. Methods: T he study employed a cross-sectional design and included 53 psoriasis patients and 47 healthy controls from December 2020 to May 2021. After DNA isolation from skin scrapings, the L1 gene region of HPV was amplified using MY09/11 and GP5 +/6 + consensus primer sequences. T he HPV genotypes were determined through direct DNA sequence analysis of polym...