Bulunan: 60 Adet 0.001 sn
Araştırmacılar Pürhan Barbaros Tuncer
Pürhan Barbaros TuncerTEKNİK BİLİMLER MESLEK YÜKSEKOKULU VETERİNERLİK BÖLÜMÜ LABORANT VE VETERİNER SAĞLIK PR.
169658

Can Plant Extracts Be Used as an Alternative for Short- and/or Long-Term Storage of Semen in Some Animal Species?

Tuncer,, Pürhan Barbaros

Ex situ koruma, genetik kaynakların ve nesli tükenmekte olan kanatlıların korunmasında önemli bir yöntemdir. Gamet koruması için kanatlılarda tek yöntem spermanın dondurulmasıdır. Çünkü kanatlı yumurtaları oldukça hacimlidir ve bunların dondurularak saklanması için kullanılan kriyoprotektanların çözüm sonu uzaklaştırılması pek mümkün değildir. Son yirmi yılda, standart kanatlı spermasının don durma teknolojisini geliştirmek için birçok çalışma yapılmıştır. Farklı ırklara uyum sağlamak için birçok girişim ve yöntem araştırılmıştır. Bu bölümde, ilk olarak spermatozoanın yapısı ve kanatlı spermasının dondurulmasını etkileyen ana faktörlerden bahsedilmiştir. Nanopartiküller ve sperma sulandırıcılarına katılan nanopartiküllerin sperma dondurulabilirliği üzerine etkileri hakkında bilgi verilmiş...

169368

Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages

Tuncer, Purhan Barbaros | Buyukleblebici Serhat

The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): ≤3years, n: 20; middle age (M): 4–6 years, n: 20; old age (O): ≥7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5mg/120×106 CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48h. Later, the frozen straws were thawed in a wa...

169369

Genomewide association of male reproductive traits in Aksaray Malakli dogs

Tuncer, Pürhan Barbaros | Büyükleblebici Serhat

Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) geno type of the investigated characteristics and reproductive loci. A total of 46 male dogs aredividedintothreeagegroups(I≤3years,n = 19; II 4–6 years; n = 19, and III ≥7years,n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The A...

169396

The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen

Tuncer, Pürhan Barbaros | Büyükleblebici, Serhat

The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose . Insemination doses were processed that each 0.25-mL straw contained 15 106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics...

169489

Değişik sulandırıcılar ile dondurulan aygır spermalarının in vitro değerlendirilmesi üzerine çalışmalar

Tuncer, P. B.

The aim of this research was to investigate spermatolojical charecteristics of stallion semen frozen with lactose and tris extenders. This experiment was done in the Anadolu State Farm and five Pure Arabian Stallions were used. Semen taken from five Arabian Stallions were extended in a way that to ensure 100x106 /cm3 motile spermatozoa by lactose and tris extenders and frozen in the macrotube in liquid nitrogen vapour. In the semen after thawing of 500C-40 seconds the average values of semen; sperm motility (%),total percentage of abnormal sperm (%), percentage of abnormal acrosome (%) and thermo-rezistance test motility (%) in 1., 2. and 3. hours were recorded; %47.44; %31.73; %22.64; %22.74; %9.13 and %2.58 respectively for frozen semen by lactose. In the semen after thawing of 500C-...

169506

Evaluationofpost-thawqualityofBrown-SwissandHolsteinbullsemendilutedwith different extenders

Tuncer,, Pürhan Barbaros

Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p

169348

Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga

There are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the d...

169484

Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stres parameters of Angora goat semen

Tuncer, P. B.

This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation, and antioxidant activities of Angora goat semen after the freeze-thawing process.

169495

Holştayn boğa spermalarının +40 C’de saklanmasında farklı sperma sulandırıcılarının motiliteye etkisi.

Tuncer, P. B.

This study was conducted to compare Laiciphose W 488 and AndroMed extenders for maintaining sperm motility at 40C in bulls. In the study 40 ejaculates collected from 4 Holstein bulls were used. Ejaculates were divided into two groups and were diluted with Laiciphos W 488 and AndroMed. Diluted semen was cooled to +40C in 45 minutes. After that they were transferred into the refrigerator. Sperm motility was estimated daily up to day 10 post dilution. Immediately after dilution sperm motility rates were 83.37±0.37 % and 83.12±0.38 % for Laiciphos W 488 and AndroMed extenders, respectively. Sperm motility for the samples diluted with AndroMed on day 3 was above 50 % in all bulls studied. However, motility rates in sperm samples diluted with Laiciphos W 488 were higher than 50% in 2 bulls onl...

169978

Boğa Spermalarının İki Farklı Sulandırıcı ile Dondurulması ve İn Vitro Değerlendirilmesi Freezing of Bull Semen with Two Different Extenders and In Vitro Evaluation

TUNCER, Pürhan Barbaros

In this study effect of AndroMed (AS) and Laiciphose W 488 (LS) extenders on the sperm motility and abnormal spermatozoa rates was examined in Holstein bull semen after they were diluted equilibrated and, thawed. In the study 40 ejaculates collected from 4 bulls were used. Averaga semen volume (8.47±0.69%), sperm concentration (x106/ml) 1030.55±7.11, and abnormal spermatozoa (3.47±0.18%) were found. The motility rates of fresh semen and after equilibration that diluted with LS and AS were 83.37±0.37%, 73.50±0.80% and 83.12±0.38%, 72.25±0.83% respectively. After thawing motility rates of sperm diluted with LS and AS extenders were 54.63±1.47%, 52.75±1.16% and, abnormal spermatozoa percentages of the sperm were 7.75±0.34% and 8.25±0.30%. According to this study, neither of these extenders a...

169380

Evaluation of some spermatological characteristics in Gerze cocks

TUNCER, Pürhan Barbaros

The aim of this research was to prove the principal spermatological characteristics in Gerze cocks in Turkey. In this experiment, 30 Gerze cocks were used. Semen was collected from cocks by abdominal massage method, two times a week during the 9 weeks period. The spermatological charecteristics of samples are as following: Ejaculate volume average 0.37±0.006 ml, spermatozoa motility 74.28±0.73%, spermatozoa concentration 2.42±0.02x109/ml, percentage of abnormal spermatozoa 6.32±0.10%, percantage of dead spermatozoa 19.74±0.73%, pH averaged 7.71±0.01 in Gerze cocks. Acrosome, head, middle piece and tail deformations of 540 ejaculates were recorded as average 0.39±0.03%, 1.06±0.03%, 2.32±0.05% and 2.53±0.04%, respectively. This is the first study which was proved some spermatological chara...

169655

The Role of Exogenous Antioxidants in Enhancing Reproductive Function and Performance

Tuncer,, Pürhan Barbaros

THE ROLE OF EXOGENOUS ANTIOXIDANTS IN ENHANCING REPRODUCTIVE FUNCTION AND PERFORMANCE IN HORSES Primary sex organs in males are the two testicles, where sperm are produced. The testicular seminiferous tubule and peritubular tissue are composed of germinal epithelium, and this tissue contains the basic cell types: somatic and germinal cells. The spermatogonial stem cells and germ cells, including differentiated cells formed during and after meiosis at dissimilar developmental degrees, are primary and secondary spermatocytes and spermatids, respectively.

169388

Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen

Tuncer, Pürhan Barbaros

This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora goat semen after the freeze–thawing process. Ejaculates collected from four Angora goats were evaluated and pooled at 37 C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant (P < 0.05) increases in sper...

169395

Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage

Tuncer, Pürhan Barbaros

This study was conducted to determine the effects of methionine, inositol and carnitine on sperm (motility, abnormality, DNA integrity and in vivo fertility) and oxidative stress parameters (lipid peroxidation, total glutathione and antioxidant potential levels) of bovine semen after the freeze–thawing process. Nine ejaculates, collected with the aid of an artificial vagina twice a week from each Simmental bovine, were included in the study. Each ejaculate, splitted into seven equal groups and diluted in Tris-based extender containing methionine (2.5 and 7.5 mM), carnitine (2.5 and 7.5 mM), inositol (2.5 and 7.5 mM) and no additive (control), was cooled to 5 C and then frozen in 0.25 ml straws. Frozen straws were then thawed individually at 37 C for 20 s in a water bath for the evaluation...

169483

The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities

Tuncer, P. B.

The aim of the present study was to determine the effects of different doses of raffinose and methionine on pot thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck sperm following cryopreservation.

169399

Effectiveness of optidyl®, bioexcell® and egg yolk tris-based extenders to freze Brown-Swiss and Holstein bull semen.

Tuncer P.B.

2010-09-30

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