Biochemical, Histopathologic, and Genotoxic Effects of Ethanol Extract of Salvia hypargeia (Fisch. & Mey.) on Incisional and Excisional Wounded Diabetic Rats
Guzel, Sevda | Uzun, Cosar | Celik, Ayla | Camlıca, Yusuf | Guler, Gizem | Erdal, Nurten | Tasdelen Bahar | Celikcan, Havva Didem
Purpose: Nonhealing wounds are a serious problem of diabetic patients. Salvia species are traditionally used for the treatment of wounds. The aim of the study was to investigate the effects of ointment prepared with ethanol extract obtained from the aerial parts of Salvia hypargeia, an endemic plant from Turkey, on diabetic rat incisional and excisional skin wounds. Materials and Methods: Male Wistar albino rats (n: 60) were divided into five groups. Diabetes was induced and two concentrations (0.5% and 1%) of the extract were used for ointments and applied on wounds for 7 and 14 days. Fito cream was chosen as a reference drug. Results: In excisional wounds, healing ratios of 0.5% (63.4% and 99.3%) and 1% (65.5% and 99.9%) S. hypargeia groups were higher compared to control (35.9% and 75.1...
The ameliorating effect of silymarin against vancomycin-induced apoptosis and inflammation in rat liver
Güzel Kara, Sevda | Uçkun Şahinoğulları, Zuhal | Canacankatan, Necmiye | Antmen, Ş. Efsun | Kibar, Deniz
Silymarin (SL), a flavonolignan complex isolated from seeds of Silybum marianum (Asteraceae), is known for its hepatoprotective, anti-apoptotic, anti-inflammatory, and antioxidant activities. A glycopeptide antibiotic, Vancomycin (VA) which is used for the treatment of serious infections caused by multi-resistant Gram-positive microorganisms has been clinically used for a long time. The aim of the present study was to evaluate potential therapeutic efficiency of SL against VA-induced apoptosis and inflammation using apoptotic (caspase-3, -8, and, -9) enzyme activities) and inflammatory (Tumor necrosis factor-alpha (TNF-α)) markers, and histopathological examinations in rat liver. A total of 49 male Wistar albino rats was divided into 7 groups including control (saline, intraperitoneally (i...
Potential protective effects of naringenin against vancomycin-induced nephrotoxicity via reduction on apoptotic and oxidative stress markers in rats
Uçkun, Zuhal | Güzel, Sevda | Canacankatan, Necmiye | Kibar, Deniz | Coşkun Yılmaz, Banu
Vancomycin (VCM), a glycopeptide antibiotic, is a drug widely used in severe infections. However, VCM induce notable nephrotoxic side effects. Naringenin (NAR) is a natural of flavonoid and are known as strongly antioxidant, nefroprotective, antiapoptotic, and anti-inflammatory. The purpose of this study was to determine the potential protective effects of NAR against VCM-induced nephrotoxicity by measuring apoptotic and oxidative stress markers and evaluating histopathological alterations in rats. For this purpose, we used male Wistar albino rats that divided into seven groups: (i) Control [saline, intraperitoneally (i.p.)], (ii) carboxymethyl cellulose (0.5% CMC, orally), (iii) VCM (400 mg/kg, i.p.), (iv) NAR100 (100 mg/kg, orally), (v) VCM + NAR25 (25 mg/kg, orally), (vi) VCM + NAR50 (5...
Verbascum inulifolium Hub.-Mor. (Scrophulariaceae) Bitkisi Üzerinde Farmakognozik Araştırmalar
Verbascum cinsi (Scrophulariaceae) dünyada 360 kadar tür ile temsil edilmekte olup bu cinse ait türler Kuzey Yarıkürede ılıman bölgelerde yayılış göstermektedir. Türkiye’de 185’i endemik olmak üzere 233 türü ve 100’den fazla hibriti bulunmaktadır. V. inulifolium türü Güney Anadolu’da yayılış gösteren endemik bir türdür. Literatürde bu türün fitokimyasal içeriği ve antioksidan, antibakteriyel ve antiviral aktiviteleri ile ilgili herhangi bir çalışma bulunmamaktadır. Bu çalışmada V. inulifolium’un toprak üstü kısımlarının metanollü ekstresi fitokimyasal açıdan incelenirken; ayrıca metanollü ve diklorometanlı ekstreler antioksidan, antibakteriyel ve antiviral etki açısından da test edilmiştir. Fitokimyasal çalışmalar, bitkinin metanollü ekstresinde Yüksek Basınçlı Sıvı Kromatografi...
Bazı Vincetoxicum N. M. Wolf (Asclepiadaceae) türleri üzerinde farmakognozik araştırmalar
Pharmacognostical investigations on some Vincetoxicum N. M. Wolf (Asclepiadaceae) species
Sevda GÜZEL ÖZAY
Vincetoxicum N.M. Wolf cinsi (Asclepiadaceae) yaklaşık 100 türü bulunan, Asya'da özellikle dağlık bölgelerde yayılış gösteren bir cinstir. Vincetoxicum familyanın Anadolu'da yetişen en zengin taksonu olup 3'ü endemik olmak üzere 10 takson ile temsil edilmektedir. Vincetoxicum türleri üzerinde yapılan çalışmalarda glikozitler, fenantroindolizidin alkaloitleri, ß-sitosterol, triterpenler, flavonoitler, saponozit ve fenolik bileşikler içerdikleri saptanmıştır. Bu bileşiklerden özellikle alkaloitlerin antiviral, antifungal, antibakteriyal, antiamoebik, sitotoksik, antitümör, antilösemik, insektisit ve insekt antifeedant aktivite gibi geniş spektrumlu biyolojik aktivitelerinin olduğu belirlenmiştir. Tez konusu olarak üçü endemik olmak üzere beş takson (V. canescens subsp. canesce...
Potential renoprotective effects of silymarin against vancomycin-induced nephrotoxicity in rats
Güzel, Sevda | Uçkun, Zuhal | Canacankatan, Necmiye | Antmen, Ş. Efsun | Kibar, Deniz | Coşkun Yılmaz, Banu
Silymarin (SLY), a flavonoid complex isolated from the seeds of Silybum marianum (Asteraceae), has antioxidant, anti-apoptotic, anti-inflammatory, and anti-lipid peroxidative effects. Vancomycin (VA), used for treating serious infections, has been associated with nephrotoxicity, which limits its use. Therefore, this study aimed to investigate the potential renoprotective effects of SLY on VA-induced nephrotoxicity using renal, apoptotic (caspase-3, caspase-8, and caspase-9 enzyme activities), and oxidative stress [nitric oxide (NO) and malondialdehyde (MDA)] markers; serum blood urea nitrogen (BUN) and creatinine levels; and histopathological examination. A total of 49 male Wistar albino rats were used (n = 7): control [saline, intraperitoneally (i.p.)], dimethyl sulfoxide (i.p.), VA [400 ...
Wound healing activity of Salvia huberi ethanolic extract in streptozocin-induced diabetic rats
Guzel Kara, Sevda | Uzun, Cosar | Celik , Ayla | Camlica, Yusuf | Guler ,Gizem | Erdal , Nurten | Tasdelen, Bahar
Objective: The aim of this study was to examine the in vivo wound healing potential of Salvia huberi Hedge (endemic to Turkey) on excision and incision wound models in diabetic rats. Method: Male Wistar albino rats, 3-4 months old and weighing 180-240g were used. The animals were randomly divided into five groups including Control, Vehicle and Fito reference, and two different concentrations (0.5% and 1% weight/weight (w/w)) of ethanol extract of Salvia huberi were investigated in both wound models on streptozocin-induced diabetic rats using macroscopic, biomechanical, biochemical, histopathological, genotoxic and gene expression methods over both seven and 14 days. Fito cream (Tripharma Drug Industry and Trade Inc., Turkey) was used as the reference drug. Results: A total of 60 rats were ...
Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats
Güzel, Sevda | Uzun, Coşar | Ülger, Mahmut | Güler, Gizem | Çelik, Ayla | Çamlıca, Yusuf
Diabetic patients suffer from persistent and non-healing wounds. Salvia species are traditionally used for the treatment of wounds and colds. The aims of the present study were to evaluate the in vivo wound healing potential, in vitro antimicrobial and antioxidant activities, and total phenolic and flavonoid contents of the aerial parts of two endemic taxa, Salvia kronenburgii Rech. f. (SK) and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica (SE). Two different concentrations (0.5% and 1% (w/w)) of ethanol extracts were investigated in incision and excision wound models on Streptozotocin-induced diabetic rats using biomechanical, biochemical, histopathological, macroscopic, and genotoxic methods for 7 and 14 days. Antimicrobial activity was evaluated against Staphylococcus au...