P.B. Tuncer, M.N. Bucak, S. Büyükleblebici, F. Kuzugüden, S. Sarıözkan, U. Taşdemir, H. Kinet
The aim of the present study was to determinate the effects of different doses of glycerol and ethylene glycol in Tris extender containing the Trehalose or cystein on post-thawed bull sperm motility and motion charecteristics following cryopreservation.
N-acetyl cysteine’s (NAC) anti-inflammatory, antioxidant and vasodilator effects are well known. However, explaining the mechanism of the effects are not sufficient. In this study, on the vasodilatory effect of NAC, role of ATP-sensitive potassium (KATP) channel was investigated by using electrophysiological and molecular genetics methodologies. In this study, aorta smooth muscle cell lines were used. One con trol and three dose groups were studied. The control group were not exposed any treatment. The dose groups were treated with 2, 5 and 10 mMNACrespectively, using the cellattached patch clamp KATP channel currents were measured for 10 min. Intracellular calcium levels in the same groups has been monitored using confocal laser scanning microscope by taking images at 488 nm wavelength w...