Cytotoxic effect of silica nanoparticles on human retinal pigment epithelial cells

Çömelekoğlu, Ülkü | Kibar, Deniz | Yıldırımcan, Saadet | Yılmaz, Şakir Necat

In recent years, the use of nanotechnology-based methods has become widespread in the treatment of ocular diseases. Silica nanoparticles (SiO2 NPs) are most common used NPs in medical field due to their physicochemical properties. SiO2 NPs can easily cross biological membranes and interact with basic biological structures, causing structural and functional changes in cells. In this study, it was aimed to investigate the dose dependent effect of SiO2 NPs on retinal pigment epithelium (RPE) in vitro using electrobiophysical, biochemical and histological methods. A commercially purchased human RPE (hARPE-19) cell line was used in this study. Cells were divided into four groups as control, 50 mg/mL SiO2, 100 mg/mL SiO2 and 150 mg/mL SiO2 groups. Cell index, apoptotic activity, cell cycle and ...