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Baspınar Nuri, Çoyan Kenan, Bucak Mustafa Numan1
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Bucak Mustafa Numan, Sarıözkan Serpil, Baspınar Nuri, Taspınar Mehmet, Çoyan Kenan, Bilgili Ali, Akalın Pınar Peker, Aydos Sena, Ilgaz Seda , Sunguroğlu Asuman, Öztuna Derya1
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Bucak Mustafa Numan, Sarıözkan Serpil, Sakin Fatih, Yeni Deniz, Ciğerci İbrahim Hakki, Atessahin , Avdate Fatihk, Gündoğan Mustafa1
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Bucak Mustafa Numan, Sarıözkan Serpil, Yeni Deniz, Eken Ayse, Akalın Pınar Peker, Kinet Hüseyin, Avdatek Fatih, Fidan A. Fatih, Gündoğan Mustafa1
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Sarıözkan S., Bucak M.N., Cantürk F., Özdamar S., Yay A., Özcan S., Sorgucu N., Caner Y.1
The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen
Tuncer, Pürhan Barbaros | Büyükleblebici, Serhat
The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose . Insemination doses were processed that each 0.25-mL straw contained 15 106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics...
The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4°C
Tuncer, P. B.
This study evaluated the protective effects of supplementation with three different sugars on the motility, morphology and DNA integrity of rat epididymal sperm chilled and stored at 4 C Epididymides were obtained from each donor. Rat epididymal sperm was diluted in Ham’s F10 plus raffinose, Ham’s F10 plus trehalose, Ham’s F10 plus fructose, and Ham’s F10 medium for control purposes. Thereafter, the extended sperm were chilled and stored in liquid form at 4 C. Sperm motility, morphological abnormalities and DNA damage were determined at 0 and 12h after chilling. No significant difference was observed in any of the parameters evaluated at 0 h, before storage (P > 0.05). After 12 h of storage, all sugar additives led to statistically higher motility, normal sperm morphology and DNA integrity...
Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage
Tuncer, Pürhan Barbaros
This study was conducted to determine the effects of methionine, inositol and carnitine on sperm (motility, abnormality, DNA integrity and in vivo fertility) and oxidative stress parameters (lipid peroxidation, total glutathione and antioxidant potential levels) of bovine semen after the freeze–thawing process. Nine ejaculates, collected with the aid of an artificial vagina twice a week from each Simmental bovine, were included in the study. Each ejaculate, splitted into seven equal groups and diluted in Tris-based extender containing methionine (2.5 and 7.5 mM), carnitine (2.5 and 7.5 mM), inositol (2.5 and 7.5 mM) and no additive (control), was cooled to 5 C and then frozen in 0.25 ml straws. Frozen straws were then thawed individually at 37 C for 20 s in a water bath for the evaluation...
The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities
Tuncer, Pürhan Barbaros | Büyükleblebici Olga
The aim of the present study was to determine the effects of different doses of raffinose and methionine on post-thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck (Capra hircus ancryrensis) sperm following cryopreservation. Ejaculates collected from three Angora bucks were evaluated and pooled at 37 C. Semen samples, which were diluted with a Tris-based extender containing the additives raffinose (2.5, 5, 10 mM) and methionine (2.5, 5, 10 mM) and an extender containing no antioxidants (control), were cooled to 5 C and frozen in 0.25 ml French straws. Frozen straws were thawed individually at 37 C for 20 s in a water bath for evaluation. The freezing extender supplemented with 2.5 and 5 mM methionine led to higher percentages of CASA motility (63.6 ± ...
Effects of dithioerythritol on ram semen after the freeze–thawing process
Tuncer, Pürhan Barbaros
The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extenderon the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2 mM dithiothreitol and no antioxidant (control), were cooled to 5 C and frozen in 0.25 ml French straws. Frozen straws were then thawed individually at 37 C for 20 s in a water bath for evaluation. The addition of dithioerythritol at 0.5 and 2 mM doses led to higher percentages of subjective motility (62.9 ± 4.2% and 63.6 ± 1.8%) compared to control (52.0 ± 4.9%, P < 0.05). As regards CASA motility, dithio erythrito...