SUPPLEMENTATION OF ROSMARINIC ACID HAS REDUCED OXIDATIVE STRESS ON BULL SPERMATOZOA FOLLOWİNG THE FREEZE THAWING PROCESS
Tuncer Pürhan Barbaros
BACKGROUND: Cryopreservation has a side effect on the motility, chromatin integrity and viability of sperm cells. OBJECTIVE: The present study investigated the effects of supplementation with rosmarinic acid (RA) Tris extender on sperm quality parameters, plasma and acrosome membrane damage, antioxidant enzyme activity and chromatin integrity following the freeze thawing process on bull spermatozoa. MATERIALS AND METHODS: Ejaculates were split into five aliquots and diluted to a final concentration of 15x106 spermatozoa/ml with the Tris extender containing RA (25, 50, 100 and 200 µg/ml) and (control) and then frozen at a controlled rate. RESULT: Treatments did not give better results on the percentages of sperm progressive, total motility and sperm motion characters (P>0.05); how...
CAN LINOLEIC ACID IMPROVE THE QUALITY OF FROZEN THAWED BULL SPERM?
Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici Olga
BACKGROUND: Cryopreservation is known to have a detrimental effect on the motility, viability and membrane integrity of sperm cells. OBJECTIVE: The aim of this study was to investigate the effect of various amount of linoleic acid supplementation to the Tris extender, on bull sperm parameters, DNA integrity and oxidative stress after freeze thawing. METHODS: Ejaculates were split into five aliquots and extended to a final concentration of 18x106 spermatozoa/ml with the base extender containing different doses of linoleic acid 0.125 ml, (L125); 0.250 ml (L250); 0.5 ml (L500), 1 ml (L1000) and no additive (control; L0). The extended samples were equilibrated slowly to 4°C for 4 h and then froze using a digital freezing machine. Frozen straws were thawed individually in water bath at 37°C...