Bulunan: 6 Adet 0.001 sn
Dergi Adı Cryobiology
169348

Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga

There are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the d...

169360

Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability

Tuncer, Pürhan B. | Büyükleblebici, Serhat

Supplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P > 0.05). For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60 ± 0.24% and 4.80 ± 0.20%), glutamine (2.80 ± 0.20% and 6.40 ± 0.40%), carnitine (2.60 ± 0.24% and 6.00 ± 0.63%) and methionine (3.40 ± 0.51% and 9.20 ± 0.86%) at doses of 2 mM provided a better protective effect, compared to that of the controls (8.00 ± 0.44 and 15.60 ± 1.895). As regards sperm membrane integrity, supplementation with 2 mM of glutamine and methionine (56.00 ±1.70% and 62.40 ±1.78%, respectively) resulted in higher rates, when compared to the control group (41.40 ± 4.74%). Accordin...

169385

The influence of cysteine and taurine on microscopic–oxidative stress parameters and fertilizing ability of bull semen following cryopreservation

Tuncer, Pürhan Barbaros

Oxidative stress significantly damages sperm functions such as motility, functional integrity, endogenous antioxidant enzyme activities and fertility due to lipid peroxidation induced by reactive oxygen species (ROS). The aim of this study was to determine the effects of antioxidants such as taurine and cysteine in Bioxcell extender on standard semen parameters, fertilizing ability, lipid peroxidation (LPO) and antioxidant activities comprising reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) after the cryopreservation/thawing of bull semen. Nine ejaculates for each bull were included in the study. Three groups, namely taurine (2 mM), cysteine (2 mM), and control, were designed to analyze the antioxidants in Bioxcell . Insemination...

169365

Influence of fetuin and hyaluronan on the post-thaw quality and fertilizing ability of Holstein bull semen

Tuncer Pürhan Barbaros | Büyükleblebici Serhat

It was determined that fetuin and hyaluronan supplementation did not provide any significant effect on the post-thaw subjective and CASA motility percentages and sperm motion characteristics, in comparison to the controls (P > 0.05). Sperm acrosome and total abnormalities were similar in all groups (P > 0.05). Groups M (hyaluro nan +fetuin) and H (hyaluronan) displayed a higher rate of sperm membrane integrity, compared to that of Group C (control) (P < 0.01). According to the results of the comet assay, the lowest percentage of sperm with damaged DNA was achieved in Group H, when compared to all of the experimental groups (P < 0.01). Furthermore, all of the additives resulted in a lower rate of sperm with damaged DNA than that of the controls, and thus, reduced DNA damage (P < 0.01). For...

169362

Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

Büyükleblebici Serhat | Tuncer P. Barbaros | Öğretmen Fatih

In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (2...

169267

Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters.
Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters.

Büyükleblebici,S. | Tuncer, P. B. | Büyükleblebici, O

Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters.