Evaluationofpost-thawqualityofBrown-SwissandHolsteinbullsemendilutedwith different extenders
Tuncer,, Pürhan Barbaros
Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p
Theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithin-based extenderonsemenqualitytofreezebullsemen
Tuncer, P. B. | Büyükleblebici S.
Aim: The centrifuged egg yolk (CEY) was added at con centrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell®) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane integrity assessed. Materials and Methods: Ejaculates obtained from each Simmental bull (n:12) divided in three equal aliquots and diluted in CEY 5% (B5), 10% (B10) supplemented, and without any CEY (B0) in soy bean lecithin - based extender, respectively. Thereafter, they were frozen and thawed following a standart protocol. Sperm cryosurvival was evaluated in vitro by microscopic assessments of post-thaw sperm motility (by means of the CASA), acrosomal and other abnormalities (head, mid-pieces, and tail) and plasma membrane inte...