Effect of α-Lipoic Acid Supplementation to Extender on Quality of Frozen-Thawed Bull Semen

BÜYÜKLEBLEBİCİ Olga | Büyükleblebici Serhat | Tuncer,, Pürhan Barbaros

This study was conducted to investigate the possible protective effect of α-lipoic acid (ALA) supplementation to extender on damages in the quality of bull semen elicited by freze-thawing process-induced oxidative stres. Ejaculates were collected via an artificial vagina from the bulls at once a week. Then they were split into five aliquots and extended with the Tris base extender containing different doses of ALA, except control. The extended samples were equilibrated slowly to 4°C for 4 h and then frozen using a digital freezing machine. Frozen straws were thawed to analyse progressive motility and sperm motility characteristics as well as plasma membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. Treatment of extender with ALA groups cause...