Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen

Tuncer, Pürhan Barbaros

This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora goat semen after the freeze–thawing process. Ejaculates collected from four Angora goats were evaluated and pooled at 37 C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant (P < 0.05) increases in sper...