Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze–thawing process
Tuncer, Pürhan Barbaros
The anti-oxidant system of reduced glutathione (GSH), glutathione peroxidase (GSH-PX), catalase (CAT), and superoxide dismutase (SOD) has been described as a defense functioning mechanism against lipid peroxidation (LPO) in semen, and is important in maintaining sperm motility and viability. This anti-oxidant capacity of sperm cells may be insufficient in preventing LPOduring the freeze–thawing process. The aim of this study was thus to determine the influence of varying doses of anti-oxidant additives on standard semen parameters, lipid peroxidation and anti-oxidant activities after the freeze–thawing of goat semen. Ejaculate samples (artificial vagina) obtained from 4 mature Angora goats were evaluated and pooled at 37◦C. The semen samples diluted with a Tris-based extender, contai...
Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation
Tuncer, Pürhan Barbaros
The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora goat semen. Ejaculates collected from three Angora goat bucks, were evaluated and pooled at 37◦C. The semen samples which were diluted with a Tris-based extender containing addi tives including glutamine (2.5; 5mM) and hyaluronan (500; 1000 l/ml), and an extender containingnoantioxidants(control)werecooledto5◦Candfrozenin0.25mlFrenchstraws and stored in liquid nitrogen. Frozen straws were thawed individually (37◦C) for 20s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5mMglutamine led to...
Comparison of the effects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities
Tuncer, Pürhan Barbaros
Ram semen contains sufficient quantities of superoxide dismutase (SOD) and much lower concentrations of glutathione peroxidase (GSH-PX) and catalase (CAT) to prevent oxidative damage. The anti-oxidant capacity of the sperm cell is limited, due to a small cytoplasmic component,which contains these anti-oxidants to scavenge the oxidants. However ,the concentration of these anti-oxidants may decrease considerably by the dilution of the semen. The aim of the present work was to study the effect of two anti-oxidants, namely ,glutamine and an amino acid solution (BME) in a Tris-based extender on ram sperm parameters, lipid peroxidation and anti-oxidant capacity after the cryopreservation/thawing process. Ejaculates collected from 4 Akkaraman rams were evaluated and pooled at 37◦C. Semen samples ...
The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities
Tuncer, Pürhan Barbaros
Theaimofthisstudywastodeterminetheeffectsoftheantioxidantscurcumin,inositoland carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora goat semen. Ejaculates were collected via artificial vagina from three Angora goats and microscopically evaluated and ooledat37◦C. The pooled semen samples were diluted in aT ris-based extender, including curcumin (2.5, 5 or 10mM), inositol (2.5, 5 or 10mM), carnitine (2.5, 5 or 10mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2–0.3◦C/min) cooled to 5◦C and then cryopreserved in 0.25mL French straws. Frozen straws were thawed individually at 37◦C for 20s in a water bath, for microscopic sperm evaluation. The freezing extender supple...