Cryoprotective effect of vaccenic acid supplemented in bull semen extender
Tuncer,, Pürhan Barbaros
The aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 µg / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 °C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by using a programmable freezer. The greatest progressive and total motility results were obtained in the VA25 group (p < 0.001). Besides, the total abnormality and chromatin damage was determined to be on the lowest level in the VA25 gr...
Antioxidant supplementation ameliorates bull sperm parameters and fertilizing ability following the freeze-thaw process
TUNCER, Pürhan Barbaros | Büyükleblebici, Serhat
This study’s goal was to reveal the effects of antioxidant supplementation on motility, motion characters, morphology, DNA integrity, and fertilizing potential of cryopreserved bovine sperm. The ejaculates were collected from three Holstein bulls. At least ten ejaculates were collected per bull. The ejaculates were immediately separated into five aliquots, diluted in the Cryobos (Magapor Co. Ltd., Zaragoza, Spain) commercial extender, including 2.5 mM taurine, 2.5 mM cysteine, 2.5 mM methionine, 2.5 mM glutamine, and no additives were used. Afterward, they were frozen in 0.25 mL French straws, and liquid nitrogen was used for the storage of semen. The supplementation of methionine resulted in a higher subjective motility rate in comparison with the other groups. Taurine led to the lowest...