Bulunan: 15 Adet 0.001 sn
Kurum Dışı Yazarlar BERKÖZ, MEHMET
167472

Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP

In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.

167438

The protective role of ferulic acid against imidacloprid-induced oxidative stress in liver and brain of Cyprinus carpio

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

This study evaluated the potential protective effect of ferulic acid (FA) on stress induced by imidacloprid (IM) in Cyprinus carpio (mean weight 60.10±2.22 g). Therefore, C. carpio was exposed to sublethal IM concentration (2.80 mg/L) for 10 days. During the experimental period, fish were fed a basal (control) diet and a basal diet supplemented with 5 g/kg of ferulic acid. The fish were divided into four groups (n = 6 in each group). The control group was exposed to tap water (the absence of imidacloprid) fed with control diet. The FA group was exposed to tap water (the absence of IM) and was fed with FA supplemented diet. The liver and brain tissues of both control and treated fish were dissected. Tissues samples were obtained from an individual fish and prepared for analysis. The variat...

167453

Prophylactic efect of myricetin and apigenin against lipopolysaccharide‑induced acute liver injury

KARAYAKAR, FAHRİ | YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

Background: Liver has an important role in the initiation and progression of multiple organ failure that occurs in sepsis. Many natural active substances can be used to reduce the liver injury caused by sepsis. For this aim, the efects of myricetin and apigenin on mice model of acute liver injury was evaluated in this study. Methods and results: Thirty-six mice were randomly divided into six groups as; control, lipopolysaccharide (LPS) (5 mg/kg), LPS+myricetin (100 mg/kg), LPS+myricetin (200 mg/kg), LPS+apigenin (100 mg/kg), and LPS+apigenin (200 mg/kg) groups. Myricetin and apigenin were administered orally for 7 days, and LPS was administered intraperitoneally only on the 7th day of the study. 24 h after LPS application, all animals were sacrifced and serum biochemical parameters, hist...

Makale2021Molecular Biology Reports 21 | 0 Erişime Açık
167452

Biochemical and Antimicrobial Properties of Alburnus tarichi Roe Protein Hydrolysate

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP

The protein hydrolysates with antioxidant and antimicrobial potential have become a theme of great interest for pharmaceutical, nutraceuticals, as well as food processing and preservation industries. On the other hand, chemical composition of fish protein hydrolysates is significant in nutrition perspective of human health. Hence, in the present study proximate and amino acid compositions, antioxidant and antimicrobial activities of Alburnus tarichi roe protein hydrolysate was estimated. Protein, lipid, moisture, and ash content were found to be 87.24±0.1, 0.72±0.04, 8.79±0.3, and 7.01±0.09%, respectively. Glutamic acid, aspartic acid, lysine, leucine and alanine were found to be the most dominant amino acids, whereas, cysteine was found to be the lowest amino acid in roe protein hydrolys...

167480

Effects of dietary selenium of organic form against lead toxicity on the antioxidant system in Cyprinus carpio

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP

In this study was evaluated potential protective effect of organic selenium (Se) on heavy metal stress induced by lead (Pb) in Cyprinus carpio. For this reason, C. carpio was exposed to sublethal concentration of Pb (1.5 mg/L Pb(NO3)2) for 14 days. The fish were fed a basal (control; measured 0.55 mg/kg Se) diet or a basal diet supplemented with 2.50 mg/kg (measured 2.92 mg/kg Se) organic Se (Sel-Plex®) during the experiment period. The variations in glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) activities, and levels of reduced glutathione (GSH) with malondialdehyde (MDA) in liver and brain tissues of C. carpio were investigated in experimental groups. GSH levels in liver and brain tissues were significantly decreased by exposure to Pb. GST activity was significantly in...

167477

The protective role of ascorbic acid (vitamin C) against chlorpyrifos-induced oxidative stress in Oreochromis niloticus

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN SERAP

Ability of ascorbic acid (vitamin C) to attenuate oxidative damage was evaluated in liver and brain tissues of Oreochromis niloticus (O. niloticus) experimentally exposed to sublethal concentrations of chlorpyrifos (CPF). O. niloticus was exposed to sublethal concentrations of CPF at 12 μg/L (CPF1) and 24 μg/L (CPF2) for 96 h. The fish of vitamin C (Vit C) and CPF2 + Vit C groups were fed with Vit C supplemented diet (200 mg Vit C/100 g feed). A significant increase in thiobarbituric acid-reactive substances (TBARS) level (P < 0.05) was observed in brain of CPF-exposed fish although liver TBARS level was not changed compared to control group. This result showed that lipid peroxidation (LPO) was elevated in brain of fish exposed to CPF. Glutathione peroxidase (GSH-Px) activity in liver and ...

167471

Investigation of Glutathion S-Transferase, Adenosine deaminase, Paraoxonase Activities in Liver of Oncorhynchus mykiss Fed with Nucleotide-Yeast Supplemented Diet

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

In this study, it was evaluated Glutathione S transferase (GST), Adenosine deaminase (ADA), and paraoxonase (PON) activities in liver tissue of Oncorhynchus mykiss fed with nucleotide yeast base protein supplemented diet. Throughout the 60-day period the control group was fed a fish meal based basal diet, and three other groups were fed diets in which 20% (NP 20), 40% (NP 40) and 60% (NP 60) fish meal was substituted with nucleotide (Nu-Pro® (NP) yeast). At the end of experiment, liver tissue GST, PON and ADA activity was increased significantly (P

166254

Effects of Dietary Nucleotide Yeast on Immune Responses and Antioxidant Enzyme Activities of Rainbow Trout Juveniles (Oncorhynchus mykiss)

HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | ENGİN, KENAN | YALIN, SERAP

This study aimed at demonstrating the effects of dietary supplementation of nucleotide yeast base protein (Nu-Pro®) (NP) on the antioxidant enzyme activities and immune response in liver and blood tissues of rainbow trout (Oncorhynchus mykiss). Fish with an average initial weight of 27.75±0.26 g were randomly assigned to four groups with three replicates. Throughout the 60 day grow-out period the control group was fed a fish meal based basal diet, and three other groups were fed diets in which 20 (NP 20), 40 (NP 40) and 60 % (NP 60) fish meal was substituted with nucleotide (Nu-Pro® (NP) yeast). There were no significant changes of superoxide dismutase (SOD) and catalase (CAT) activities in liver among the experimental groups. A significant decrease (P

167475

Organik ve İnorganik Selenyum Katkılı Yemlerle Beslenen Oreochromis niloticus ‘da Antioksidan ve İmmün Sistem Parametrelerinin Araştırılması

HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | DELİOĞLAN, ŞANSER

Bu çalışmada organik (Sel-Plex®-2000, Alltech, USA) ve inorganik selenyum (Na2SeO3.5H2O -Sodyum selenite pentahydrate-FLUCA) ilaveli yem ile beslenen Oreochromis niloticus’un karaciğer antioksidan enzim aktiviteleri ve immünsistem parametreleri araştırılmıştır. Ortalama ağırlıkları 12,62 ± 0,11 g olan balıklar, 5 farklı uygulama grubu (Kontrol; Org1,5; Org3,0; İnorg1,5; İnorg3,0) olacak şekilde akvaryumlara yerleştirilmiş ve 75 gün beslenmişlerdir. Deneme süresi sonunda karaciğer katalaz (CAT), süperoksit dismutaz (SDO), glutatyon peroksidaz (GSH-Px), lizozim (LYZ) ve miyeloperoksidaz (MPO) aktiviteleri ile malondialdehit (MDA) düzeyi ve kas selenyum seviyeleri belirlenmiştir. Karaciğer antioksidan enzim aktiviteleri ve immünsistem parametreleri Se gruplarında kontrol grubuna oranla artmış...

166296

The effects of fish meal replacement by yeast based nucleotides on growth body composition and digestive enzyme activity in rainbow trout juveniles Oncorhynchus mykiss

HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | ENGİN, KENAN | YALIN, SERAP | ŞAHİN, NEFİSE ÖZLEN

This 60 day study investigated the effects of organically certified nucleotide yeast-derived protein source (Nu-Pro®) on growth, feed efficiency, fillet proximate composition and digestive enzymes in rainbow trout (Onchorhyncus mykiss). Diets were isonitrogenous and isocaloric. Three experimental diets wherein 20%, 40% and 60% of the fish meal content was replaced by Nu-Pro®, were compared to the control diet in which the crude protein content was anchovy fish meal and corn gluten meal. The rainbow trout (initial weight 27 g/fish) were fed twice daily in 200-l Aqaria. Live weight gain increase ranged from 125-195% in fish fed the experimental diets. Results indicated that up to 40% fish meal protein can be replaced by Nu-Pro® without compromising growth rates, feed efficiency or the fillet...

167486

Effects of Organic Selenium on Growth, Muscle Composition, and Antioxidant System in Rainbow Trout

HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | YALIN, SERAP

This experiment was conducted to understand the effects of organic selenium on rainbow trout, Oncorhynchus mykiss. Fish (33.47±0.15 g; n = 216) were randomly assigned to four treatment groups consisting of three replicates of 18 fish each in 100 x 100 x 125 cm cages. The fish were fed a basal diet supplemented with 2, 3, or 4 mg/kg organic Se (Sel-Plex®) supplementation for 8 weeks. Muscle of fish fed the unsupplemented control diet had a lower (p

167479

Induction of micronuclei and other nuclear abnormalities in peripheral erythrocytes of Nile tilapia, Oreochromis niloticus, following exposure to sublethal cadmium doses

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

The genotoxic effects of sublethal doses of cadmium in peripheral erythrocytes of Oreochromis niloticus was investigated using micronucleus (MN) and nuclear abnormalities (NAs) tests. For this reason, the fish were exposed to medium changes of 0.5 and 1.0 mg/L doses of cadmium (Cd) during an average treatment period of 10 days. On the 2nd, 4th, 6th, and 10th days of the test period, the erythrocytes, including the micronuclei (MN) and other nuclear abnormalities such as lobbed nuclei (LB), blebbed nuclei (BL), and notched nuclei (NT), were counted and pointed out. As a positive control, a 4 mg/L dose of cyclophosphamide was used. Exposure to doses of cadmium caused significantly increased (P < 0.05) micronuclei in peripheral erythrocytes of fish. It was also observed that the application o...

167484

Effect of Mannan Oligosaccharide on Growth Body Composition and Antioxidant Enzyme Activity of Tilapia Oreochromis niloticus

HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | YALIN, SERAP

The effects of three inclusion levels of mannan oligosaccharides (MOS) derived from the outer cell wall of a select strain of Saccharomyces cerevisiae (Bio-Mos, Alltech Inc, USA) on growth, feed utilization, body composition, and antioxidant enzyme activity of tilapia (Oreochromis niloticus) were determined. Specimens (12 g) were fed a commercial diet supplemented with 0‰, 2.5‰, 3.5‰, or 4.5‰ dietary MOS for 60 days. Food conversion rates (FCR), specific growth rates (SGR), and the biochemical composition of muscle tissue were determined. Growth was greatest, protein was highest, and lipid was lowest in fish fed the 2.5‰ MOS diet. The FCR was significantly better in all MOS-treated groups than in the unsupplemented control. At the end of the study, antioxidant enzyme activity of superoxid...

167465

Influence of Sublethal Chlorpyrifos Exposure on Oxıdative Stress and Acetylcholinesterase Activity in Common Carp (Cyprinus carpio)

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP

The commonly used pesticides in agriculture cause enzyme inactivation and DNA damage via reacting with macromolecules and may also initiate peroxidation of poly-unsaturated fatty acids (PUFA) by generating of the reactive oxygen species (ROS), as a consequence they can lead to the oxidative stress. The objective of this study was to determine the oxidative and neurotoxic potential of sublethal chlorpyrifos in the brain tissue of Cyprinus carpio (C. carpio) for a period of 96 and 240 hours, respectively. Therefore, the levels of malondialdehyde (MDA), glutathione (GSH), advanced oxidation protein products (AOPP), protein carbonyl, and 8-hydroxydeoxyguanosine (8-OHdG) levels and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE...

167455

Roe protein hydrolysate of Alburnus tarichi induces apoptosis in breast cancer MCF-7 and MDA-MB-231 cells through a caspase-dependent pathway

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

The protein hydrolysates of fishes have been reported to be a potential source of many health benefits components for pharmaceutical or nutritional applications. The aim of this study is to examine the possible antiproliferative function of roe protein hydrolysates of Alburnus tarichi using enzymatic hydrolysis against breast cancer cells and explore its detailed mechanisms. In addition, we evaluated the effects of protein hydrolysate on the proliferation and apoptosis of two human breast cancer cell lines (MCF-7 and MDA-MB-231). The cultured cells were treated with protein hydrolysate at concentrations of 0−5 μg/ml for 24 h and 48 h. Inhibition of cell proliferation, percentage of apoptotic cells, cell cycle distribution, morphological changes, DNA fragmentation, intracellular reactive o...