PPAR-g2 Pro12Ala polymorphism is associated with weight gain in women with gestational diabetes mellitus
Tok, Devrim | Erdal, Mehmet Emin
Objective: The polymorphisms of peroxisome proliferator–activator receptor-g2 (PPAR-g2) have been suggested to affect glucose metabolism and weight gain. Both conditions show great variations during pregnancy that makes pregnancy a suitable condition to detect any metabolic abnormalities related to PPAR-g2 polymorphisms. The objective of this study is to investigate the prevalence and metabolic impacts of PPAR-g2 polymorphism in control pregnant women and in patients with gestational diabetes mellitus (GDM). Methods: In this case-control study, anthropometric and metabolic variables of 100 non-diabetic pregnant women and of 62 women who were diagnosed as having GDM according to 100 g oral glucose tolerance test (OGTT) were compared on the basis of PPAR-g2 polymorphism by univariate analysi...
Association of insulin receptor substrate-1 G972R variant with baseline characteristics of the patients with gestational diabetes mellitus
Tok, Devrim | Erdal, Mehmet Emin
Objective: Insulin receptor substrate-1 (IRS-1) expression and tyrosine phosphorylation is decreased during pregnancy. Pregnancy may be a suitable condition to detect any abnormalities related to IRS-1 polymorphisms. Therefore, we aimed to investigate the prevalence and metabolic impacts of IRS-1 G972R polymorphism in patients with gestational diabetes mellitus (GDM). Study design: Anthropometric and metabolic variables of 62 women who were diagnosed as having GDM according to 100 g oral glucose tolerance test were compared on the basis of IRS-1 polymorphism by univariate analysis of covariance. Results: Patients with IRS-1 G972R were more obese at the beginning of pregnancy, had higher serum fasting insulin and glucose levels. Weight gain during pregnancy and insulin and glucose levels af...
Evaluation of glucose metabolism and reproductive hormones in polycystic ovary syndrome on the basis of peroxisome proliferator-activated receptor (PPAR)-g2 Pro12Ala genotype
Tok, Devrim | Erdal, Mehmet Emin
BACKGROUND: Peroxisome proliferator-activated receptor (PPAR)-g2 Pro12Ala polymorphism has been suggested as a protective factor for polycystic ovary syndrome (PCOS). In this study, we aimed to investigate metabolic features and reproductive hormones in women with PCOS and compare these features with control women on the basis of Pro12Ala genotype. METHODS: This study involved 60 randomly selected women with PCOS and 60 controls. Main outcome measures were anthropometric measures, variables of glucose metabolism and reproductive hormones. All the patients were genotyped for Pro12Ala variant of PPAR-g2 gene. RESULTS: Patients with Pro12Ala polymorphism were more obese in both groups. Furthermore, they had lower fasting insulin levels, were less insulin-resistant and were less glucose-intole...
Association of Gly972Arg variant of insulin receptor substrate-1 with metabolic features in women with polycystic ovary syndrome
Tok, Devrim | Erdal, Mehmet Emin
Objective: To examine the prevalence and the effects of Gly972Arg (G972A) variant of insulin receptor substrate-1 (IRS-1) in women with polycystic ovary syndrome (PCOS). Design: Controlled clinical study. Setting: An academic clinical research center. Patient(s): Sixty women with PCOS and 60 control women matched for age. Intervention(s): Biometric measures, metabolic and hormonal measures, genetic analyses. Main Outcome Measure(s): Serum androgens, glucose, and insulin were measured. Blood leukocytes were used for genetic analyses. Result(s): The G972A variant was encountered more often in women with PCOS. The G972A carriers were more obese than their counterparts, had higher fasting insulin levels, and were more insulin-resistant. However, androgen levels did not differ on the basis of I...
The importance of IRS-1 Gly972Arg polymorphism in evaluating the response to metformin treatment in polycystic ovary syndrome
Tok, Devrim | Erdal, Mehmet Emin
BACKGROUND: Recent evidence suggests that one of the modes of action of metformin may be through phosphorylation of the insulin receptor and insulin receptor substrates. With this in mind, we supposed that the G972A variant of insulin receptor substrate-1 (IRS-1) may modulate the response to metformin treatment in women with polycystic ovary syndrome (PCOS). METHODS: This preliminary study involved 60 randomly selected women with PCOS. All patients received dietary instructions and metformin 500mg three times daily for 6 months. Main outcome measures were androgen levels, parameters of glucose and insulin metabolism and anthropometric variables. After a second evaluation of the patients at 6 months, they were genotyped for the Gly972Arg variant of the IRS-1 gene. RESULTS: Metformin had dif...