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Fakülteler10
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Tıp Fakültesi10
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Temel Tıp Bilimleri Bölümü10
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Biyofizik Anabilim Dalı10
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Tıbbi Biyoloji ve Genetik Anabilim Dalı6
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Cerrahi Tıp Bilimleri Bölümü3
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Kadın Hastalıkları ve Doğum Anabilim Dalı3
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Biyoistatistik ve Tıbbi Bilişim Anabilim Dalı3
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İnsan ve Toplum Bilimleri Fakültesi2
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Arap Dili ve Edebiyatı Bölümü2
Lack of Effect of Extremely Low Frequency Electromagnetic Fields on Cyclin-Dependent Kinase 4 Inhibitor Gene p18INK4C in Electric Energy Workers.
Erdal, Nurten | Erdal, Mehmet Emin
Background. Long-term exposure to extremely low frequency magnetic fields (ELF-MFs) may be a risk factor for human cancer. One mechanism through which ELF-MFs could influence neoplastic development is the deletion/mutation of cancer-related genes. Cellular proliferation follows an orderly progression through the cell cycle, which is governed by different cyclins and cyclin-dependent kinase inhibitors (CDKIs). The putative tumor suppressor gene p18INK4C encodes a specific inhibitor of cyclin D-cyclin-dependent kinase 4 inhibitor complexes having an important role in cell-cyclin regulation. It has been found to be deleted/mutated in a variety of human cancers. Therefore, this study is to investigate whether or not long-term extremely low frequency electromagnetic field exposure may be a risk...
Yemler Bilgisi Yem Teknolojisi ve Balık Besleme
Bilgüven, Murat
Her havuz alanında ya da yetiştirme biriminden elde edilen balık ve dolayısıyla balık yetiştiriciliğinin kârlılığı, büyük ölçüde kullanılan yemin miktarına bağlıdır. Su ürünleri sistemi ne kadar yoğun olursa yemlemenin önemi o kadar fazla olur ve toplam üretim maliyetinde yem maliyetinin payı da o kadar artar. Bilindiği üzere alabalık başta olmak üzere bazı balıkların yetiştiriciliği için akarsu sistemli tesisler kullanılmakta yada sıcaklığın, su bileşiminin ve akıntının dolayısıyla oksijen yoğunluğunun daha iyi olduğu tatlı su ve kıyılarda ise yüzer ağ kafeslerde yetiştiricilik yapılmaktadır. Su ürünleri yetiştiriciliğinin ileri durumda olduğu ülkelerde entansif yetiştiricilik uygulanmakta ve yetiştirme alanlarında birim hacimde maksimum ürün yetiştirilmeye çalışılmaktadır. Bu tür yetişti...
Effects of electromagnetic radiation on neurogenesis and gene expression in amniocytes,
Erdal, Nurten | Ay, Özlem İzci | Yıldırım, Didem Derici | Durukan, Hüseyin | Erdal, Mehmet Emin
The aim of the study was to investigate the genetic effects of commonly used radiofrequency on the amniocytes of pregnant women by examining the changes in the expression of genes related to signal transduction and neurogenesis induced by electromagnetic fields of radiofrequencies of 1800 and 2100 MHz by real-time PCR. Upon exposure of the amniocytes to electromagnetic radiation of 2100 MHz at high field strength, the expression levels of genes with important functions in the Wnt signaling pathways as well as neurogenesis-related genes were increased. The findings suggest that radiofrequency at high field strengths can have genotoxic effects during pregnancy.
N -Acetylcysteine-induced vasodilatation is modulated by K ATP channels, Na /K -ATPase activity and intracellular calcium concentration: An in vitro study
Çömelekoğlu, Ülkü | Sucu, Nehir | Yalın, Ali Erdinç | Yılmaz, Şakir Necat | Yalın, Serap | Söğüt, Fatma | Kibar, Kezban | Akkapulu, Merih
Background In this study, we aimed to investigate the role of ATP-sensitive potassium (KATP) channel, Na+/K+-ATPase activity, and intracellular calcium levels on the vasodilatory effect of N-acetylcysteine (NAC) in thoracic aorta by using electrophysiological and molecular techniques. Methods Rat thoracic aorta ring preparations and cultured thoracic aorta cells were divided into four groups as control, 2 mM NAC, 5 mM NAC, and 10 mM NAC. Thoracic aorta rings were isolated from rats for measurements of relaxation responses and Na+/K+-ATPase activity. In the cultured thoracic aorta cells, we measured the currents of KATP channel, the concentration of intracellular calcium and mRNA expression level of KATP channel subunits (KCNJ8, KCNJ11, ABCC8 and ABCC9). Results The relaxation rate sig...
Investigation of the role of KATP channels in the cytotoxic effect of cypermethrin on rat-derived aortic smooth muscle cells
Söğüt, Fatma | Kibar, Deniz | Çömelekoğlu, Ülkü
We investigate role of atP sensitive potassium (KatP) channel in cytotoxic effect of cypermethrin on rataortic smooth muscle cells. cytotoxicity analysis was performed at 0, 0.1, 0.5, 10, 50, and 100 µMconcentrations of cypermethrin and the cell index (ci) was calculated. KatP currents were recorded usingpatch clamp technique for 50 and 100 µM concentrations and channel conductivity was determined byobtaining current-voltage characteristics. No cytotoxic effect was observed in the first 72 hours. at the96th hour, only at 100 µM concentration, the ci value decreased significantly compared to control groupand at 120 and 144th hours, it was observed that the ci value decreased significantly at all concentrations.currents and conductivities were significantly decreased at 50 and 100 µM concent...
Investigation of the role of K-ATP channels in the cytotoxic effect of cypermethrin on rat-derived aortic smooth muscle cells
Söğüt, Fatma | Uzun, Coşar | Kibar, Deniz | Çömelekoğlu, Ülkü
We investigate role of ATP sensitive potassium (KATP) channel in cytotoxic effect of cypermethrin on rat aortic smooth muscle cells. Cytotoxicity analysis was performed at 0, 0.1, 0.5, 10, 50, and 100 µM concentrations of cypermethrin and the cell index (CI) was calculated. KATP currents were recorded using patch clamp technique for 50 and 100 µM concentrations and channel conductivity was determined by obtaining current-voltage characteristics. No cytotoxic effect was observed in the first 72 hours. At the 96th hour, only at 100 µM concentration, the CI value decreased significantly compared to control group and at 120 and 144th hours, it was observed that the CI value decreased significantly at all concentrations. Currents and conductivities were significantly decreased at 50 and 100 µM ...
Role of antioxidant defense system and biochemical adaptation on stress tolerance of high mountain and steppe plants
Keleş, Yüksel
Eleven species were collected from Northwest Anatolian mountains (1500–2000 m) and 18 species were collected from the Central Anatolian steppes (850–1000 m) in June 1998 and 1999. In all the species investigated, the water and dry matter percentages and solute contents were measured. The chlorophyll, b-carotene, ascorbate and a-tocopherol contents and catalase (CAT), superoxide dismutase (SOD) andglutathione reductase (GR)enzymeactivities oftheplants werealsodetermined.Thesteppeplantshadlowerwatercontentcomparedwith alpine plants. The chlorophyll contents of the plants investigated did not change with altitude. However, the carotenoid/chlorophyll ratio of alpine plants was found to be significantly higher. The antioxidant/chlorophyll ratio of the trees and shrubs was higher than that of he...
The A218C polymorphism of tryptophan hydroxylase gene and migraine
Erdal, Nurten | Erdal, Mehmet Emin
Objective: To determine the significance of the A218C polymorphism of the tryptophan hydroxylase (TPH) gene in migraine. Methods: Fifty-nine migraineurs and 62 healthy controls were included in the study, and polymerase chain reaction – restriction fragment length polymorphism assays were used to determine TPH A218C polymorphism. Results: There was no association between TPH gene polymorphism and gender, family history of migraine and epilepsy, or aura. There was no significant difference between the allele frequencies of both groups (p > 0.05). A significant difference was found between the genotypes of the migraineurs and controls regarding the AA genotype. Homozygosity for the C allele or heterozygosity for the A or C was not associated with the occurrence of migraine (p > 0.05), but ho...
Role of 2.4 GHz radiofrequency radiation emitted from Wi-Fi on some miRNA and faty acids composition in brain
Erdal, Nurten | Erdal, Mehmet Emin
The purpose of this study is to investigate the effects of 2.4 GHz Wi-Fi exposure, which is continuously used in the internet connection by mobile phones, computers and other wireless equipment, on microRNA and membrane and depot fatty acid composition of brain cells. Sixteen Wistar Albino rats were divided equally into two groups such as sham and exposure. The rats in the experimental group (n = 8) were exposed to 2.4 GHz RFR emitted from a Wi-Fi generator for 24 h/ day for one year. The animals in the control group (n = 8) were kept under the same conditions as the experimental group, but the Wi-Fi generator was turned off. At the end of the study, rats were sacrificed and brains were removed to analyze miRNA expression and membrane and depot fatty acids of brain cells. We analyzed the s...
Cytotoxic effect of silica nanoparticles on human retinal pigment epithelial cells
Çömelekoğlu, Ülkü | Kibar, Deniz | Yıldırımcan, Saadet | Yılmaz, Şakir Necat
In recent years, the use of nanotechnology-based methods has become widespread in the treatment of ocular diseases. Silica nanoparticles (SiO2 NPs) are most common used NPs in medical field due to their physicochemical properties. SiO2 NPs can easily cross biological membranes and interact with basic biological structures, causing structural and functional changes in cells. In this study, it was aimed to investigate the dose dependent effect of SiO2 NPs on retinal pigment epithelium (RPE) in vitro using electrobiophysical, biochemical and histological methods. A commercially purchased human RPE (hARPE-19) cell line was used in this study. Cells were divided into four groups as control, 50 mg/mL SiO2, 100 mg/mL SiO2 and 150 mg/mL SiO2 groups. Cell index, apoptotic activity, cell cycle and ...