Biochemical, Histopathologic, and Genotoxic Effects of Ethanol Extract of Salvia hypargeia (Fisch. & Mey.) on Incisional and Excisional Wounded Diabetic Rats
Guzel, Sevda | Uzun, Cosar | Celik, Ayla | Camlıca, Yusuf | Guler, Gizem | Erdal, Nurten | Tasdelen Bahar | Celikcan, Havva Didem
Purpose: Nonhealing wounds are a serious problem of diabetic patients. Salvia species are traditionally used for the treatment of wounds. The aim of the study was to investigate the effects of ointment prepared with ethanol extract obtained from the aerial parts of Salvia hypargeia, an endemic plant from Turkey, on diabetic rat incisional and excisional skin wounds. Materials and Methods: Male Wistar albino rats (n: 60) were divided into five groups. Diabetes was induced and two concentrations (0.5% and 1%) of the extract were used for ointments and applied on wounds for 7 and 14 days. Fito cream was chosen as a reference drug. Results: In excisional wounds, healing ratios of 0.5% (63.4% and 99.3%) and 1% (65.5% and 99.9%) S. hypargeia groups were higher compared to control (35.9% and 75.1...
Wound healing activity of Salvia huberi ethanolic extract in streptozocin-induced diabetic rats
Guzel Kara, Sevda | Uzun, Cosar | Celik , Ayla | Camlica, Yusuf | Guler ,Gizem | Erdal , Nurten | Tasdelen, Bahar
Objective: The aim of this study was to examine the in vivo wound healing potential of Salvia huberi Hedge (endemic to Turkey) on excision and incision wound models in diabetic rats. Method: Male Wistar albino rats, 3-4 months old and weighing 180-240g were used. The animals were randomly divided into five groups including Control, Vehicle and Fito reference, and two different concentrations (0.5% and 1% weight/weight (w/w)) of ethanol extract of Salvia huberi were investigated in both wound models on streptozocin-induced diabetic rats using macroscopic, biomechanical, biochemical, histopathological, genotoxic and gene expression methods over both seven and 14 days. Fito cream (Tripharma Drug Industry and Trade Inc., Turkey) was used as the reference drug. Results: A total of 60 rats were ...
Role of 2.4 GHz radiofrequency radiation emitted from Wi-Fi on some miRNA and faty acids composition in brain
Erdal, Nurten | Erdal, Mehmet Emin
The purpose of this study is to investigate the effects of 2.4 GHz Wi-Fi exposure, which is continuously used in the internet connection by mobile phones, computers and other wireless equipment, on microRNA and membrane and depot fatty acid composition of brain cells. Sixteen Wistar Albino rats were divided equally into two groups such as sham and exposure. The rats in the experimental group (n = 8) were exposed to 2.4 GHz RFR emitted from a Wi-Fi generator for 24 h/ day for one year. The animals in the control group (n = 8) were kept under the same conditions as the experimental group, but the Wi-Fi generator was turned off. At the end of the study, rats were sacrificed and brains were removed to analyze miRNA expression and membrane and depot fatty acids of brain cells. We analyzed the s...
Effects of electromagnetic radiation on neurogenesis and gene expression in amniocytes
Erdal, Nurten | Ay, Özlem İzci | Yıldırım, Didem Derici | Durukan, Hüseyin | Erdal, Mehmet Emin
The aim of the study was to investigate the genetic effects of commonly used radiofrequency on the amniocytes of pregnant women by examining the changes in the expression of genes related to signal transduction and neurogenesis induced by electromagnetic fields of radiofrequencies of 1800 and 2100 MHz by real-time PCR. Upon exposure of the amniocytes to electromagnetic radiation of 2100 MHz at high field strength, the expression levels of genes with important functions in the Wnt signaling pathways as well as neurogenesis-related genes were increased. The findings suggest that radiofrequency at high field strengths can have genotoxic effects during pregnancy.
Lack of Effect of Extremely Low Frequency Electromagnetic Fields on Cyclin-Dependent Kinase 4 Inhibitor Gene p18INK4C in Electric Energy Workers.
Erdal, Nurten | Erdal, Mehmet Emin
Background. Long-term exposure to extremely low frequency magnetic fields (ELF-MFs) may be a risk factor for human cancer. One mechanism through which ELF-MFs could influence neoplastic development is the deletion/mutation of cancer-related genes. Cellular proliferation follows an orderly progression through the cell cycle, which is governed by different cyclins and cyclin-dependent kinase inhibitors (CDKIs). The putative tumor suppressor gene p18INK4C encodes a specific inhibitor of cyclin D-cyclin-dependent kinase 4 inhibitor complexes having an important role in cell-cyclin regulation. It has been found to be deleted/mutated in a variety of human cancers. Therefore, this study is to investigate whether or not long-term extremely low frequency electromagnetic field exposure may be a risk...
Monoamine oxidase-A gene promoter polymorphism in temporomandibular joint pain and dysfunction
Erdal, Mehmet Emin | Erdal, Nurten
Objective: In this study we aimed to evaluate the relationship between temporomandibular joint pain and dysfunction (TMJPD) within the frame of monoamine oxidase-A gene (MAO-LPR) gene polymorphism. Methods: Ninety-three patients with TMJPD, and 91 healthy volunteers were included in the study. Symptom Checklist-90-Revised (SCL-90-R), Montgomery-Asberg Depression Rating Scale (MADRS), and State and Trait Anxiety Inventory tests (STAI-I and STAI-II) were applied to the patients that were diagnosed as having TMJPD according to the standard clinical examination protocol. Blood samples were taken from the patients, and the variability in the MAO-A gene was analyzed by amplification of DNA with polymerase chain reaction (PCR) for a genetic association study. Results: The analysis of allele and g...
The A218C polymorphism of tryptophan hydroxylase gene and migraine
Erdal, Nurten | Erdal, Mehmet Emin
Objective: To determine the significance of the A218C polymorphism of the tryptophan hydroxylase (TPH) gene in migraine. Methods: Fifty-nine migraineurs and 62 healthy controls were included in the study, and polymerase chain reaction – restriction fragment length polymorphism assays were used to determine TPH A218C polymorphism. Results: There was no association between TPH gene polymorphism and gender, family history of migraine and epilepsy, or aura. There was no significant difference between the allele frequencies of both groups (p > 0.05). A significant difference was found between the genotypes of the migraineurs and controls regarding the AA genotype. Homozygosity for the C allele or heterozygosity for the A or C was not associated with the occurrence of migraine (p > 0.05), but ho...
T102C Polymorphisms at the 5-HT2A Receptor Gene in Turkish Schizophrenia Patients: A Possible Association with Prognosis
Erdal, Mehmet Emin | Erdal, Nurten
Background: Serotonergic system abnormalities have been implicated in the pathogenesis of schizophrenia. The 5-HT2A receptor gene polymorphism has long been implicated to play a role in the pathogenesis of schizophrenia. Aim: In this study, we assessed the relationship of schizophrenia and its subgroups with 5-HT2A receptor gene polymorphism, and attempted to evaluate a possible correlation between the severity and prognosis of the illness and 5-HT2A receptor gene polymorphism. Method: Our study comprised 141 unrelated subjects who strictly met DSM-IV criteria for schizophrenia, and 79 healthy unrelated controls, all of Turkish origin. A clinical evaluation of all patients was accomplished applying the Brief Psychiatric Rating Scale (BPRS) test. The analysis of 5-HT2A receptor gene polymor...
Association Analysis of the Functional MAOA Gene Promoter and MAOB Gene Intron 13 Polymorphisms in Tension Type Headache Patients
MEHMET EMİN ERDAL | AYNUR ÖZGE | NURTEN ERDAL
Abstract Background. Monoamine oxidase (MAO) enzymes play an important role in the etiology of many neurological diseases. Tension type headache (TTH) treatments contain inhibitors for selective re-uptake of serotonin and monoamine oxidase inhibitors. MAO (EC 1.4.3.4) has two isoenzymes known as MAOA and MAOB. A promoter polymorphism of a variable number of tandem repeats (VNTR) in the MAOA gene seems to affect MAOA transcriptional activity in vitro. Also, G/A polymorphism in intron 13 (rs1799836) of the MAOB gene have been previously found to be associated with the variability of MAOB enzyme activity. Objectives. The aim of our study was to investigate a possible association of monoamine oxidase (MAOA and MAOB) gene polymorphisms in tension type headache. Material and Methods. MAO gene po...
Effects of electromagnetic radiation on neurogenesis and gene expression in amniocytes,
Erdal, Nurten | Ay, Özlem İzci | Yıldırım, Didem Derici | Durukan, Hüseyin | Erdal, Mehmet Emin
The aim of the study was to investigate the genetic effects of commonly used radiofrequency on the amniocytes of pregnant women by examining the changes in the expression of genes related to signal transduction and neurogenesis induced by electromagnetic fields of radiofrequencies of 1800 and 2100 MHz by real-time PCR. Upon exposure of the amniocytes to electromagnetic radiation of 2100 MHz at high field strength, the expression levels of genes with important functions in the Wnt signaling pathways as well as neurogenesis-related genes were increased. The findings suggest that radiofrequency at high field strengths can have genotoxic effects during pregnancy.