Epidemiological and molecular characterization of Enterobius vermicularis cases in Mersin, Turkey: An analysis based on mitochondrial DNA cox1 gene sequences
Gülbudak Harun | Tezcan Ülger Seda | Bozok Taylan | Aslan Gönül
Enterobius vermicularis is among the most common intestinal helminths, especially in children. Mitochondrial cox1 gene analyses revealed three genotypes (A, B, C). We aimed to evaluate the epidemiological characteristics and cox1 genotypes of E. vermicularis cases diagnosed at Mersin University Hospital. Between January 2017 and December 2023, 1496 samples were examined using the cellophane tape method, and 75 positives were identified. Epidemiological data were retrospectively analyzed. DNA was isolated from 30 positive samples via the phenol–chloroform method, and nested PCR products of the cox1 gene were sequenced. Phylogenetic analyses were performed using GenBank sequences. In a sample of patients with gastrointestinal signs, the positivity rate for E. vermicularis was 5 % (75/1496)...
Pneumocystis jirovecii İzolatlarının Dihidropteroat Sentaz (DHPS) Gen Mutasyonları ve Mitokondriyal Büyük Alt Birim (mtLSU) rRNA Genotip Varyasyonlarının Belirlenmesi
Gülbudak, Harun | Öztürk, Candan | Ülger, Seda Tezcan
Pneumocystis jirovecii, HIV/AIDS ve immün sistemi baskılanmış hastalarda Pneumocystis pnömonisine (PCP) neden olan atipik bir fungustur. PCP profilaksi ve tedavisinde sülfa ve sülfon grubu içeren ilaçlar yaygın olarak kullanılır. Özellikle trimetoprim sulfametoksazol (TMP-SMX)’un, uzun süreli kullanılması P.jirovecii dihidropteroat sentaz (DHPS) geninde ilaç direnci ile ilişkili belirli nokta mutasyonlara neden olmaktadır. Ayrıca, DHPS ve mitokondriyal büyük alt birim [mitochondrial large subunit (mtLSU)] rRNA genotip karakterizasyonu P.jirovecii epidemiyolojisi hakkında önemli veri sağlamaktadır. Bu çalışmada, Mersin Üniversitesi Hastanesinde, immün sistemi baskılanmış hastalardan izole edilen P.jirovecii izolatlarının DHPS ve mtLSU rRNA gen bölgesi polimorfizmlerinin araştırılması amaçla...
Evaluation of cutaneous leishmaniasis cases in Mersin, Türkiye: Impact of migration and the COVID-19 pandemic
Gülbudak Harun | Bozok Taylan | Tezcan Ülger Seda | İkizoğlu Güliz | Delialioğlu Nuran | Aslan Gönül
Introduction: Cutaneous leishmaniasis (CL) is a significant public health concern in Türkiye, especially in the Southeastern Anatolia and Mediterranean regions. This study evaluated the epidemiological characteristics of CL cases diagnosed at Mersin University Hospital, including the impact of migration and the COVID-19 pandemic. Methodology: The diagnosis of CL was confirmed by microscopic examination of lesion smears. In this study, data from 144 patients diagnosed with CL between January 2011 and December 2022 were evaluated retrospectively. Demographic characteristics of the patients, temporal distribution (by month and year), and lesion features (location, number, and duration) were included. Results: CL was confirmed in 32.9% of patients (144/438). Of the 144 patients, 82 (56.9...