MicroRNAs (miRNAs) are a new endogenous small non-coding RNA family that arranges expression of a few genes related in normal development as well as human diseases such as cancer. miRNAs are developing as potential diagnostic and therapeutic markers with deregulated expression in various cancers including lung cancer. In this study, we aimed to find out miRNA expression profiles and to show the relationship between non-small cell lung cancer and microRNAs levels, if there is. In the present study, the expression profiles of 740 miRNA in plasma from 31 patients and 64 healthy subjects were evaluated using high-throughput real-time quantitative polymerase chain reaction. All statistical analyses were performed using the Biogazelle's qbase PLUS 2.0 software. Our results showed that expression levels of 7 microRNAs (miR-20b-5p, -30c, -146a, -192-5p, - 206, -484, -574-3p) were significantly upregulated in patients when compared to control group (p< 0.05). Expression levels of five miRNAs (miR-24-3p, - 30a-5p, -106b-5p, -223-3p, -331-5p) were found significantly downregulated in lung cancer patients (p< 0.05). Conclusion: In conclusion, our result showed that up-regulated and down-regulated miRNAs may be important role of early detection in non-small cell lung cancer.