The objective of this study was to evaluate glycerol (G), ethylene glycol (EG) and dimethylsulfoxide (DMSO) which were used two different doses on in vitro semen parameters, antioxidant enzymes activities and DNA damage after the freeze-thaw process in Angora goat semen. Semen samples from 5 mature Angora goats were used in this study. A total number of 40 ejaculates were collected twice a week from the
goats using an artificial vagina and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 6 equal aliquots and diluted with tris base extenders supplemented with two different doses of cryoprotectants (G 3%, 6%; EG 3%, 6%; DMSO 3%, 6%). G 3% and 6% was added as a cryoprotectant had better CASA motility (P<0.01) and progressive motility (P<0.001) values when compared to EG and DMSO groups. On the other hand, EG 6% showed the best values of preserved membrane integrity (P<0.01). The evaluation of CASA sperm motions parameters, adverse effects were procured in the groups with DMSO groups when compared to the other groups (P<0.05; P<0.001). G 6% group was the greatest VAP, VSL and VCL values than the other groups (P<0.05; P<0.001). DNA damage was not affected by supplemented different doses of cryoprotectants as well as antioxidant activity (P>0.05). In conclusion, no advantages were found in using EG or DMSO to replace G for freezing of Angora goat sperm.