Harvesting impacts the costs of microalgae production and affects the characteristics of
the final product. Therefore, this study evaluated Moringa oleifera seed powder (MP) as a
bioflocculant compared to two chemicals (Aluminium Sulphate—AS and Iron Chloride—IC) to
harvest a mixed microalgae culture (Chlorella vulgaris and Desmodesmus sp.) grown on digestate. MP
was the most stable flocculant but resulted in the lowest harvesting efficiency of 75%, compared to
94% for AS and 100% for IC. Process parameters such as pH, duration of mixing, grinding method
for obtaining the powder, and granulometry had no significant effect on the harvesting efficiency
of MP, reinforcing that this is a robust flocculant. The use of a water extraction step increased the
harvesting efficiency of MP to 91%, albeit with the need for a higher dosage of flocculant. The algae
harvested with MP complied with maximum tolerable levels for swine, cattle, and poultry
regarding most trace elements. Nevertheless, all algae samples had Fe and Al contents above the
recommended levels, possibly due to the entrapment of metal‐rich digestate particles. Therefore,
more attention should be paid to the final composition of algae when proposing flocculation as a
harvesting method for feed production.