Mutlu Nisa Ünaldı Coral Mutlu Nisa Ünaldı Coral EĞİTİM FAKÜLTESİ MATEMATİK VE FEN BİLİMLERİ EĞİTİMİ BÖLÜMÜ FEN BİLGİSİ EĞİTİMİ ANABİLİM DALI
Araştırmacının yayınlarını daha detaylı bir şekilde aramak veya filtrelemek için buraya tıklayabilirsiniz.

Öğretmen Adaylarının Fen Bilimleri Eğitiminde Sanal Tasarım ve Üç Boyutlu Yazıcı Entegrasyonunun İncelenmesi

Koç, Adem | Coral, Mutlu Nisa Ünaldı

Fen bilimleri içeriğinde soyut kavramların oldukça yoğun olduğu bir öğrenme alanıdır. Konu ve kavramlara göre somutlaştırma işlemleri geleneksel laboratuvar ve fiziksel tasarım atölyelerinde gerçekleştirilebilse de, bu her zaman mümkün olamamakta ya da daha zor olabilmektedir. Bu sorunların üstesinden gelebilmek ve öğrenmede somutlaştırmanın önemine vurgu yapmak için, bu çalışma fen bilgisi öğretmen adaylarının katılımı ile soyut kavramların çoğunlukta olduğu Biyoloji III dersi kapsamında gerçekleştirilmiştir. Haftalık olarak ikişer ders saati şeklinde mühendislik tasarım süreci aşamalarına ve proje tabanlı öğrenme yöntemine göre gerçekleştirilen bu uygulamada üç boyutlu tasarım programı olan Tinkercad programı ve üç boyutlu yazıcı kullanılmıştır. Uygulama süreci başında öğretmen adayları araştırmacılar tarafından ayrıntılı olarak bilgilendirilerek sanal ortamda tasarıma ilişkin çalışma yaprakları sunulmuştur. Haftalık belirlenen plan çerçevesinde uygulamalar biyoloji ve bilgisayar laboratuvarlarında gerçekleştirilmiştir. 3-5’er kişilik gruplar halinde çalışan öğretmen adayları seçtikleri konuda gerçekleştirdikleri bireysel tasarımlarından sonra bir tasarıma karar verip, onu nihai tasarım olarak geliştirmişlerdir. Süreç sonunda her bir grubun nihai tasarımı üç boyutlu yazıcıda ba...

Development and Validation of the Metacognitive Awareness Scale for Learning with Multimedia

Koç, Adem | Coral, Mutlu Nisa Ünaldı

Technology has become increasingly prevalent in the field of education. Alongside the growing presence of technology in educational environments, the importance of learning with multimedia tools has also increased. It is believed that individuals need to be aware of how to learn most effectively in these environments for effective learning to take place. This research aims to develop a valid and reliable scale to reveal university students' metacognitive awareness of learning processes with multimedia. To achieve this goal, the study was conducted in two phases. Firstly, a pool of items was created by reviewing the literature, and 407 data were obtained to explore this pool of items. Subsequently, to confirm the structure of the revealed 6-factor scale, 318 data were collected from a second study group. The confirmed scale consists of a total of 41 items under six factors, explaining 54% of the total variance. The reliability coefficients for internal consistency of the scale as a whole were calculated as [omega] = 0.90 and [alpha] = 0.85. The results of the study indicate that the developed scale is a valid, reliable, and effective tool for measuring university students' metacognitive awareness of learning with multimedia.

Fen Bilimleri Öğretmenlerinin Biyoteknoloji Öğretimi Konusundaki Görüşlerinin İncelenmesi

Coral, Mutlu Nisa Ünaldı

Bireylerin biyoteknoloji alanında yeterli seviyeye ulaşabilmeleri ancak iyi bir öğretim programı ve biyoteknoloji konusuna hâkim fen bilimleri öğretmenleri ile gerçekleşebilir. Bu çalışmanın amacı, ortaokulda görev yapan fen bilimleri öğretmenlerinin biyoteknoloji öğretimine ilişkin görüşlerini belirlemektir. Buna göre araştırma, nitel araştırma yöntemlerinden durum çalışması olarak desenlenmiştir. Araştırmanın çalışma grubu Mersin ilinin Akdeniz, Tarsus ve Yenişehir ilçelerinde görev yapan toplam 20 fen bilimleri öğretmeninden oluşmaktadır. Çalışma grubunun seçiminde olasılık temelli olmayan örnekleme yöntemlerinden uygun örnekleme yöntemi kullanılmıştır. Araştırmanın verileri 2021-2022 eğitim öğretim yılında elde edilmiştir. Araştırma sonuçlarına göre öğretmenler, fen bilimleri dersi öğretim programının biyoteknoloji konu ve kazanımlarına yeterince yer vermediğini belirtmişlerdir. Ayrıca öğretmenler fen bilimleri dersi öğretim programında biyoteknoloji öğretimi için ayrılan ders süresinin yeterli olmadığını belirtmişlerdir. Program hazırlayıcıların fen bilimleri öğretim programlarında güncel biyoteknoloji konu ve kazanımlarına yer vermeleri ayrıca biyoteknoloji öğretimi için ayrılan ders süresinin arttırılması önerilmektedir.

Isolation and characterization of lytic bacteriophages infecting fish pathogen Vibrio sp. strains

Talaş, Gizem | Coral, Gökhan | Coral, Mutlu Nisa Ünaldı | Yılmaz, Şakir Necat

In this study, bacteria were isolated from infected organs of sea bass (Dicentrarhus labrax) and identified as Vibrio sp. according to 16S rRNA analysis. Four bacteriophages were isolated from seawater samples collected from the coast of Mersin province in Turkey.Vibrio sp. strains were used as hosts for bacteriophage isolation. Bacteriophages inhibit the growth of their hosts; Vibrio sp. K12, Vibrio sp. K11, Vibrio sp. L8, Vibrio sp. L5 which are potential bacterial pathogen of sea bass. Bacteriophages were characterized by Restriction Analysis (RA) and Transmission Electron Microscopy (TEM). Restriction Analysis showed that genomes of bacteriophages LP8, KP12, LP5 and KP11 are double stranded DNAs and the sizes of these genomes are 73, 55, 44 and 23 kb respectively. Only three out of phage were visualized by TEM. Electron microscopy studies revealed that bacteriophages LP5 and LP8 have an icosahedral head with a contractile tail and belonged to family Myoviridae. Bacteriophage KP11 shows binary symmetry, and belongs to family Siphoviridae with a long noncontractile tail. Experiments were carried out with three phages which were characterized by TEM. One-step growth experiments showed that the phages have different latent periods (30-40-50 min) and burst sizes (58-123-141) plaqu...

Çevrimiçi ölçme ve değerlendirmeye yönelik özyeterlik ölçeğinin geliştirilmesi

Koç, Adem | Uzun, Nezaket Bilge | Coral, Mutlu Nisa Ünaldı

Araştırma uzaktan eğitimde öğretici konumunda bulunanların çevrimiçi ölçme ve değerlendirmeye yönelik özyeterlik düzeylerini açığa çıkarmaya yönelik geçerli ve güvenilir bir ölçme aracı geliştirmek amacıyla gerçekleştirilmiştir. Bu amaca yönelik olarak yazılan 70 madde dokuz uzmanın değerlendirmesine sunularak kapsam geçerliği sağlanmaya çalışılmıştır. Hesaplanan kapsam geçerlik oranı ve kapsam geçerlik indeksleri sonucunda ölçek deneme formunda 49 madde yer almasına karar verilmiş ve ölçek çevrimiçi ortamda öğretmen ve öğretim elemanlarının gönüllü olarak cevaplamalarına sunulmuştur. 316 veri ile gerçekleştirilen açımlayıcı faktör analizi sonucunda ölçeğin iki bileşen altında gruplanan ve toplam 25 madde ile temsil edilen yapısına karar verilmiştir. Ölçeğin karar verilen yapısının sınanması amacıyla 202 veri üzerinden doğrulayıcı faktör analizi gerçekleştirilmiştir. Gerçekleştirilen doğrulayıcı faktör analizi sonucunda maddelere ilişkin standartlaştırılmış yük değerlerinin 0,43 ile 0,87 arasında değişkenlik gösterdiği, tüm t değerlerinin istatistiksel olarak anlamlı olduğu ve hesaplanan uyum indekslerinin de mükemmel/kabul edilebilir düzeyde olduğu görülmüştür. Ölçeğin açıkladığı toplam varyans oranı %65,895 ve ölçeğe ilişkin hesaplanan Cronbach Alpha güvenirlik katsayısı 0,958 ...

FEN BİLİMLERİ ÖĞRETMENLERİNİN OKULDIŞI (DOĞA DENEYİMİNE DAYALI) EĞİTİME YÖNELİK GÖRÜŞLERİNİN DEĞERLENDİRİLMESİ

Coral, Mutlu Nisa Ünaldı

Bu çalışmanın amacı Fen Bilimleri Öğretmenlerinin okul dışı (doğa deneyimine dayalı) eğitime yönelik görüşlerini ortaya koymaktır.Araştırmanın çalışma grubunu elverişli örnekleme metoduyla belirlenen 20 fen bilimleri öğretmeni oluşturmaktadır. Veri toplama aracı olarak araştırmacı tarafından hazırlanan ve 6 açık uçlu sorudan oluşan yarı yapılandırılmış görüşme formu kullanılmıştır. Görüşmeler her bir öğretmenle yaklaşık yirmişer dakikalık oturumlar şeklinde gerçekleştirilmiş ve ses kaydı alınmıştır. Ayrıca araştırmacı görüşme boyunca görüşme formu üzerine de notlar almıştır.Elde edilen verilerin dijital ortama aktarılmasının ardından verilere içerik analizi uygulanmış, kodlar ve temalar belirlenmiştir. Elde edilen bu kod ve temalar çerçevesinde öğretmen görüşleri saptanmış ve değerlendirilmiştir. Çalışma sonucunda öğretmenlerin okul dışı (doğa deneyimine dayalı) öğrenme ortamlarını gerekli bulmalarına rağmen, çeşitli engel ve zorluklar sebebiyle sıklıkla uygulayamadıkları sonucuna ulaşılmıştır. Bu sonuçlardan yola çıkılarak bir takım önerilerde bulunulmuştur.

Doğa Deneyimine Dayalı Çevre Eğitimi”Ne Yönelik Özyeterlik Algısı Ölçeğinin Geliştirilmesi: Geçerlik Ve Güvenirlik Çalışması

ÜNALDI CORAL MUTLU NİSA

Doğa deneyimine dayalı çevre eğitimi yaklaşımı çevreyi yerinde öğrenme imkanı sunarak anlamlı öğrenmeye katkıda bulunmasının yanı sıra çevre eğitimi dersini duvarların sınırlayıcılığından kurtarmaktadır. Bir öğretmenin doğa deneyimine dayalı çevre eğitimlerine yönelik özyeterlik algılarının yüksek olduğu koşullarda, öğretim sürecinde okul dışı ortamları kullanmaktan kaçınmayacakları söylenebilir. Bu çerçevede, öğretmen adaylarının çevre eğitimi konusundaki yeterlik algılarının belirlenmesinin alana önemli bir katkı sağlayacağı düşünülmektedir. Bu çalışmada öğretmenlerin ve öğretmen adaylarının Doğa Deneyimine Dayalı Çevre Eğitimi’ne yönelik özyeterlik algılarını ortaya koymak amacıyla bir özyeterlik algısı ölçeği geliştirilmiştir. 44 maddeden oluşan deneme ölçeği Akdeniz bölgesinde yer alan iki devlet üniversitesinin Eğitim Fakültesi üçüncü ve dördüncü sınıflarında öğrenim gören ve “ Çevre Eğitimi” dersini alan 348 öğretmen adayına uygulanmıştır. Varimax Döndürme yöntemiyle gerçekleştirilen açımlayıcı faktör analizi sonucunda üç alt boyuta sahip 20 maddelik bir yapı ortaya konmuştur. Alt boyutlar “Planlama”, “Uygulama” ve “Değerlendirme” olarak adlandırılmıştır. Ölçeğin bütününe ve alt boyutlara ilişkin Cronbach alfa güvenirliği sırasıyla, 0,921; 0,850 ; 0.793 ve 0.878’dir. AF...

Effect of Domoic Acid on Growth and Heavy Metal Tolerance of Some Marine Bacteria Isolated from Samandag Shore and Iskenderun Bay

Coral, Mutlu Nisa Ünaldı

In the current study, effects of domoic acid on some marine bacteria were studied. Domoic acid is a neurotoxin which affects human and animals and by some marine microalgea. For this study, heterotrophic marine bacteria were isolated from Iskenderun Bay and Samandag shore. 19 Pseudomonas sp. strains, 15 Acinetobacter sp. strains, 5 Alteromonas sp. strains and 9 Vibrio sp. strains were identified. For inhibition tests, varing concentrations of domoic acid (0.5µgr.ml-1 and 1 mgr.ml-1) were tested. It was found that there is not any inhibition effect of domoic acid on the all bacteria with used concentrations. Stimulator effect was determined by getting growth curve from the bacteria cultures which was added varying concentrations of domoic acid. It was found that 2.5 µgr.ml-1 and 5 µgr.ml-1 domoic acid showed inducer effect on 1.6 Pseudomonas sp. strain and 4.4 Vibrio sp. strain growth. In this study, it was also investigated the effect of domoic acid on heavy metal tolerance of these bacteria. The results of these study will contribute novel information on the effect of domoic acid on marine bacteria.

Incidence of inducible bacteriophage in Bacillus sp. And Pseudomonas sp. Strains isolated from contaminated soil in Mersin

Coral, Mutlu Nisa Ünaldı

In the current study, the effects of environmental agents on induction of soil lysogenic bacteria were studied. To estimate the prevalence of lysogeny within bacterial communities from contaminated soil, induction assays were carried out by extracting bacteria from soil. A series of experiments were conducted with samples collected in Mersin to assess the impact of some physical and chemical agents on propage induction in natural populations. A total of 90 bacteria were isolated from these sites. UV radiation, temperature, some mutagens and hydrocarbons were used to induce prophage in lysogenic bacteria. Two-thirds of the lysogenic bacteria showed prophage induction by one of the agents. The effects of these agents on the efficiency of plating (EOP) and on the adsorption of lytic bacteriophages were also investigated.

Potential neoplastic effects of parathion-methyl on rat liver

Coral, Mutlu Nisa Ünaldı

The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonella typhimurium TA100 bacterial cells was observed without rat liver S9 metabolic activation. Parathion-methyl was further investigated for pathological changes in rat pancreas and liver. The long-term rat experiments showed that parathion-methyl exposure for 3 months can cause pathological changes in rat pancreases acinar cells and pancreatic hepatocytes. Atypical acinar cell focuses (AACF) were determined in the liver and pancreas of the rats. The results from short-term Ames test and long-term rat experiments suggested that parathion-methyl would be potential carcinogenic.

Detection of polycyclic aromatic hydrocarbon (PAH) degradation genes in polluted soil by DNA extraction and colony hybridization

Coral, Gökhan | Coral, Mutlu Nisa Ünaldı

The aim of this study is to detect the DNA sequences for the PAH-dioxygenase gene, the key enzyme of the aeго-bic catabolic for polycyclic aromatic hydrocarbon degra-dation, in soil microorganisms by using extraction of total DNA, PCR amplification PAH-dioxygenase sequences, and detection with colony hybridization. To determine whether bacteria containing PAH-dioxygenase gene actually exist in environments contaminated by crude oil or not, the ge-nomic DNA was purified using a genomic DNA purifica-tion kit. Purified genomic DNA was then amplified with a thermal cycler, and the PCR products were separeted by agarose gel electrophoresis. Pseudomonas sp. strain ARP 26, which is capable of degrading phenanthrene, was used as target for colony hybridization. It was confirmed that Pseudomonas sp. strain ARP 26 demonstrated DNA se-quence homology to the digoxigenin-labeled DNA probes used

A preliminary study on tellurite resistance in Pseudomonas spp. isolated from hospital sewage

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A total of 48 Pseudomonas spp., isolated from the Çukurova University Balcalı Hospital sewage in Adana, were characterized on the basis of morphological, cultural and biochemical characteristics. To improve our understanding of the ecology of tellurite resistance in bacteria, the minimum inhibition concentrations (MIC) of potassium tellurite (K2 TeO3 ) for growth were used to determine metal tolerance of the isolated strains. Most of the strains tolerated to 55 µgr/ml potassium tellurite. Ostrain Ps 37 tolerated relatively high concentrations of tellurite (80 µgr/ml). 27% of the strains possessed plasmid mediated tellurite resistance (Telr ).

Plasmid mediated heavy metal resistances in Enterobacter spp. isolated from Sofulu landfill, in Adana, Turkey

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A total of 15 Enterobacter spp. were isolated from water drainage near the landfill in Sofulu village in Adana and were characterised on the basis of morphological, cultural and biochemical characteristics. The Maximum Tolerable Concentrations of cadmium, copper, chromium and nickel for growth were used to determine metal tolerance of the isolated strains. Among the resistant strains used in this study, Ent-5, Ent-7 and Ent-15 were found to have plasmid resistance to copper and nickel. One resistant strain, Ent-5, functioned as a donor of copper resistance. Copper resistance transferred from Ent-5 to Escherichia coli AB3505 at a frequency of approximetely 2.7 x 10-5 per recipient cell. Transformant strain had one plasmid with the same molecular weight donor strain’s plasmid. These isolates can act reservoirs of heavy metal resistance genes that could be transferred to the other bacteria.

Thermostable alkaline protease produced by an Aspergillus niger strain

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A thermostable alkaline protease was isolated from the Aspergillus niger Z1 strain in a liquid Czapek Dox medium, containing casein (1% w/v) as the sole nitrogen source. Enzyme extract was subjected to electrophoresis in SDS-polyacrylamide gel. Two protein bands were seen on polyacrylamide gel. Active enzyme was visualized in a zymogram and protease activity exhibited a molecular mass of 68 kDa on SDS-polyacrylamide gel. The optimum pH for activity was found to be 9.0. The temperature optima of the enzyme was found to be 40 °C at pH 9.0 and it remained stable up to 90 °C, with 48.4% of the original activity retained after heat treatment at 90 °C for 15 min. Proteolytic activity was inhibited by PMSF, but slightly inhibited by SDS.

Electrofusion of Saccharomyces cerevisiae Auxotrophic Mutants of Identical Mating Type Using a Laboratory-Made System

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

Yeast protoplasts from haploid auxotroph strains DC6 (MATα) and FY73 (MATα) were exposed to an inhomogeneous alternating field (AC 200 V/cm). Due to the dielectrophoretic aggregation two or more cells with close membrane contact were formed between the two electrodes. The effects of different DC pulses were investigated to determine critical fusogenic pulse strength. In the results, the maximum fusant cells were obtained only when 8 kV field strengths were applied. These findings showed that the optimum fusogenic pulse strength for yeast protoplasts is 8 kV/cm under our experimental conditions. After fusion, the DNA contents of both hybrid and individual parental strains were compared. The amount of DNA in the hybrid was found to be about twofold higher than that in the individual parental strain.

Keratinolytic activity of Streptomyces strain BA7, a new isolate from Turkey

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A wild type Streptomyces strain BA7 which was isolated from soil, showed a high keratinolytic activity when cultured on native feather medium. Optimum keratinolytic activity was observed at 50 ºC and pH 8.5 when using fluid supernatant obtained from aerated culture of this organism. The keratinolytic activity was completely stable (100%) between 30 and 60 ºC. The molecular weight of crude enzyme was estimated by SDS-PAGE about 44000 Da. The degradation of intact feathers by Streptomyces sp. BA7 keratinase was obtained after 24 h of incubation at 50 ºC. Keratinolytic activity was partially inhibited by 1,10-phenanthroline, but slightly inhibited by CaCl2, ZnCl2, and SDS. In addition keratinolytic activity was enhanced by DMSO, EDTA, Triton-X 100 and sodium sulphite. Streptomyces strain BA7 could be a useful in biotechnology in therms of valorization of keratin-containing wastes or in the leather industry

Plasmid-Encoded Heavy Metal Resistance in Pseudomonas sp.

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

No abstract available

Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A thermostable alkaline α-amylase producing Bacillus sp. ANT-6 was isolated from soil samples. Enzyme synthesis occurred at temperatures between 25 and 45 °C with an optimum of 37 °C. There was a slight variation in amylase synthesis within the pH range 7 and 11 with an optimum pH of 9. The optimum temperatures for amylase production and growth were the same. Analyses of the enzyme by sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed a single band, which show amylolytic activity, detected in starch gel. The relative molecular mass of the partial purified enzyme was estimated to be 94 500 Da. The enzyme showed optimum activity at pH 10.5 and 80 °C. The partial purified enzyme was highly active in the alkaline range of pH (9.5–13), and it was completely active up to 100 °C retaining 85.5% initial activity at pH 10.5. Enzyme activity was enhanced in the presence of 5 mM CaCl2 (110%) and 3 mM PMSF (103%), and inhibition with 5 mM by Zn, Na, Na-sulphide, EDTA (10 mM), Urea (8 M) and SDS (0.1%) was obtained 36.9, 21.5, 22.2, 4.90, 86% and 10.27, respectively. The enzyme was stable (55%) at high alkaline pH for 24 h. So our result showed that the enzyme was both alkaline, thermostable, thermopile and chelator resistant. The ANT-6 amylase enzyme may be suitable in lique...

Isolation of alcohol tolerant, osmotolerant and thermotolerant yeast strains and improvement of their alcohol tolerance by UV mutagenesis

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

In this study, the yeast strains were isolated from grapes by serial dilution technique to determine their alcohol-, sugar-and thermotolerance. 34 wild type yeast strains were isolated and alcohol-, sugar-and thermotolerance of these strains were determined. The maximum alcohol tolerance was found to be 9%(v/v) in yeast strain which is named Y2. Thermotolerance behavior of 6 strains were investigated. The strains were treated with UV light with intervals of 20, 30, 40 and 50 seconds. Selected resistant colonies were investigated for alcohol tolerance. It was found that alcohol tolerance increased from 9%(v/v) to 12%(v/v) on Y2 strain.

Some properties of crude carboxymethyl cellulase of Aspergillus niger Z10 wild-type strain

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40 °C. It was found that the enzyme's activity has a broad pH range between 3 to 9 and 41.2% of the original activity was retained after heat treatment at 90 °C for 15 min.

Some properties of thermostable xylanase from an Aspergillus niger strain

Coral, Mutlu Nisa Ünaldı | Coral, Gökhan

A thermostable xylanase was isolated from an Aspergillus niger wild type strain in a liquid Czapek Dox medium, containing oat spelts xylan as the sole carbon source. The molecular mass of the enzyme was estimated to be about 36 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum pH for activity was found to be 7.5. The temperature optimum of the enzyme was found to be 60 °C at pH 7.5. The enzyme remained stable up to 100 °C, yet lost about 50% of its activity after 15 min at this temperature.

Separation of megabased-sized DNA molecules of Aspergillus niger using pulsed field gel electrophoresis

Coral, Gökhan | Coral, Mutlu Nisa Ünaldı

In this study, the chromosomal DNAs were extracted from Aspergillus niger Z10 wild type strain and these DNAs were separated using the contour clamped homogeneous electric field gel electrophoresis (CHEF) system. This system is laboratory-made and is operated by a computer program. Total DNAs resolved into five distinct chromosomal bands. The size of the chromosomes was estimated as being between 3.3 Mb to 6.4 Mb.