Effects of Chromium on the Hematocrit Levels and Erythrocyte Numbers of Cyprinus carpio
Cicik, Bedii | Çiftçi, Nuray | Ay, Özcan
Changes in hematocrit levels and erythrocyte numbers of Cyprinus carpio were determined after exposing the animals to 0.5, 1.0 and 2.0 ppm chromium over 7, 15 and 30 days. Microhematocrit and hemocytometric methods were used for determining hematocrit levels and erythrocyte numbers respectively. No mortality was observed during the experiments. Hematocrit levels and erythrocyt numbers increased under the effect of metal on day 15. Hematocrit levels and erythtrocyte numbers decreased by increasing exposure periods except at 0.5 ppm chromium.
BAKIR, ÇİNKO, KURŞUN ve KADMİYUMUN Oreochromis niloticus' da BAZI SERUM BİYOKİMYASAL PARAMETRELER ÜZERİNE ETKİLERİ
Çiftçi, Nuray | Karayakar, Fahri | Ay, Özcan | Cicik, Bedii
Araştırmada bakır (Cu), çinko (Zn),kurşun (Pb) ve kadmiyum (Cd)’un 96 saatlik LC50 değerinin %10’unakarşılık gelen sırasıyla 4.0, 6.0, 0.2 ve 1.6 ppm ortam derişimlerinin 7, 15 ve30 gün sürelerle etkisine bırakılan Oreochromis niloticus’un serumglukoz, total protein, albumin, D-Bilirubin, aspartat aminotransferaz (AST),alanin aminotransferaz (ALT) ve total kolesterol düzeylerinde meydana gelendeğişimler belirlenmiştir. Denemesüresince incelenen derişimlerin etkisinde mortalite gözlenmemiştir. İncelenenmetallerin belirlenen süreler sonundaki etkisinde kontrole oranla, totalprotein, albumin ve D-Bilirubin düzeylerinde önemli bir ayırım saptanmazken,serum glukoz, AST, ALT ve total kolesterol düzeylerinde artış gözlenmiştir.Elde edilen bulgular, ağır metal etkisinin serum biyokimyasal parametr...
Accumulation and Effect of Chromium on Protein and Glycogen Levels of Palaemonetes pugio
Çiftçi, Nuray | Karaytuğ, Sahire | Karayakar, Fahri | Ay, Özcan | Cicik, Bedii
Total metal accumulation and the levels of total protein and glycogen were measured in Palaemonetes pugio after exposing the animals to 0.05, 0.1 and 0.2 ppm concentrations of chromium over 1, 7 and 15 days. Metal accumulation in tisuues was measured using atomic absorbtion spectrophotometric techniques and the levels of total protein and glycogen were determined Lowry and Anthron methods, respectively. No mortality was observed under the effect of chromium at any concentrations and exposure periods tested. Total metal accumulation increased with increasing concentrations of chromium at given exposure period while total protein and glycogen levels showed a decrease on day 15th compared with day 1.
Accumulation of copper in liver, gill and muscle tissues of Anguilla anguilla (Linnaeus, 1758).
Karayakar, Fahri | Cicik, Bedii | Ciftci, Nuray | Karaytug, Sahire | Ay, Özcan
Accumulation of copper in liver, gill and muscle tissues of Anguilla anguilla was studied after exposing the animals to 1.00, 2.00 and 4.00 ppm Cu over 96 h period. The levels of metals in the studied tissues were determined using atomic absorption spectrophotometric techniques. All the copper concentrations tested increased the tissue levels of the metal significantly in liver and gill tissues compared to control fish while metal accumulation in muscle tissue was statistically significant only at 4.00 ppm Cu. Accumulation of the metal in gill tissue showed no difference between the concentrations tested despite the differences observed in liver and muscle tissues. Accumulation of the metal was highest in liver tissue and lowest in muscle tissue.
Accumulation of Chromium in Liver Gill and Muscle Tissues of Orechromis niloticus
Çiftçi, Nuray | Cicik, Bedii | Ay, Özcan
The aim of the present study was to determine the levels of chromium in the liver, gill and muscle tissues of Oreochromis niloticus after exposing the animals to 1.0, 2.0 and 4.0 ppm chromium over 96 h. Metal levels in the tissues studied were determined using atomic absorbrtion techniques. Although, some behavioral disturbances were observed in the chromium exposed fish at the beginning of experiments, no mortalities were observed at the end of 96 h. Exposure to chromium increased tissue levels of the metal except the muscle tissue while tissue accumulation increased with increasing concentration of the metal in the medium. Maximum accumulation was observed in the gill tissue at the lowest concentration tested, whereas accumulation was higher in the liver tissue at 2.0 and 4.0 ppm ...
EFFECTS OF LEAD CONCENTRATIONS ON SERAPARAMETERS AND HEMATOCRIT LEVELS IN Anguilla anguilla Linnaeus 1758
Çiftçi, Nuray | Cicik, Bedii | Ay, Özcan
The effects of lead on sera glucose, protein and cholesterol concentrations and on hematocrit levels of Anguilla anguilla exposed to 0.06 and 0.12 ppm lead were studied over 15 and 30 days. Sera parameters and hematocrit levels were determined by spectrophotometric and microhematocrit methods, respectively. No mortality was observed during the experiments. Sera glucose and protein levels showed a linear increase and a decrease respectively under the effect of lead. Sera cholesterol levels decreased compared to control over 15 days of exposure to both concentrations and this decrease was not significant between the concentrations on day 30. Lead concentrations 0.12 ppm lead did not cause any variation in hematocrit level, while 0.06 ppm lead increased it compared to control.. Changes...
EFFECTS OF COPPER AND LEAD ON SOME HEMATOLOGICAL PARAMETERS OF Oreochromis niloticus
Çiftçi, Nuray | Karayakar, Fahri | Ay, Özcan | Cicik, Bedii
Effects of sub-lethal concentrations of copper and lead on hematocrit and mean cell volume (MCV) levels and on erythrocyte numbers, erythrocyte and erythrocyte nucleus areas were determined after exposing Oreochromis niloticus to 4 ppm Cu and 0.2 ppm Pb over 7, 15 and 30 days. Micro-hematocrit methods were adopted in determining hematocrit levels and microscopic methods were used to determine MCV, erythrocyte numbers, erythrocyte and erythrocyte nucleus areas. Hematocrit and MCV levels and erythrocyte and nucleus areas increased significantly (P
Bakır ve Kurşunun Oreochromis niloticus’da Hepatosomatik İndeks, Gonadosomatik İndeks ve Kondüsyon Faktörü Üzerine Etkileri
Çiftçi, Nuray | Ay, Özcan | Karayakar, Fahri | Cicik, Bedii
Araştırmada bakır (Cu) ve kurşun (Pb)’un 96 saatlik LC50 değerinin %10’una karşılık gelen derişimlerinin (sırasıyla 4,0 ve 0,2 ppm) 7, 15 ve 30 gün sürelerde etkisine bırakılan Oreochromis niloticus’un Hepatosomatik İndeks (HSI), Gonadosomatik Indeks (GSI) ve Kondüsyon Faktörü (CF) üzerine etkisinin belirlenmesi amaçlanmıştır. Belirtilen parametrelerin hesaplanmasında standart matematiksel formüller kullanılmış, verilerin istatistiksel analizinde, Student Newman Keul’s testi ile varyans analizi uygulanmıştır. Denemede Cu etkisine bırakılan balıklarda HSI’de kontrole göre artış, GSI ve CF’nde deneme süresi sonunda azalma saptanırken, Pb etkisine bırakılan balıklarda HSI’de kontrole göre azalma (P0,05). Oreochromis niloticus was exposed to 10% of its 96 h LC50 (4.0 ppm Cu and 0.2 ppm P...
Heavy metal levels in five species of fish caught from Mersin Gulf.
Karayakar, Fahri | Karaytug, Sahire | Cicik, Bedii | Ay, Özcan | Ciftci, Nuray
The aim of the present study was to determine the levels of cadmium, copper, zinc and chromium in the liver, gill and muscle tissues of some pelagic, benthopelagic and benthic fish species caught from Mersin Gulf and sold at Karaduvar Harbor. Pelagic species Sardinella aurita (Clupeidae), Liza ramado (Mugilidae), benthopelagic species Diplodus annularis (Sparidae) and benthic species Solea lutea (Soleidae) and Umbrina cirrosa (Sciaenidae) were selected as the study material. Atomic absorption spectrophotometric methods were applied in determining the tissue levels of metals. Metal levels showed tissue-dependent changes in the species studied. Pb and Cr levels were below detection limit; hence no comparison was made among the tissues. Cd, Cu and Zn levels were highest in the liver tissues a...
EFFECTS OF ZINC AND CADMIUM ON CONDITION FACTOR, HEPATOSOMATIC AND GONADOSOMATIC INDEX OF Oreochromis niloticus
Çiftçi, Nuray | Ay, Özcan | Karayakar, Fahri | Cicik, Bedii
Effects of Zn and Cd on condition factor (CF), hepatosomatic (HIS) and gonadosomatic (GSI) of Oreochromis niloticus, after exposing the animals to 10 % of their LC50 values, namely 6.0 ppm Zn and 1.6 ppm Cd, over 7, 15, and 30 days. Standard mathematical formulations were used in determining the mentioned parameters. Cadmium, which is a toxic metal, increased GSI and decreased HSI and CF at the beginning of the experiments. The decrease in HSI and CF continued with increasing exposure periods. Zinc, however, decreased all three parameters compared with control at the end of experiments (P
Effects of Cadmium and Cadmium Chitosan Mixture on Some Blood Parameters of Oreochromis niloticus
Çiftçi, Nuray | Karayakar, Fahri | Ay, Özcan | Karaytuğ, Sahire | Cicik, Bedii
Effects of 1 mgL-1 cadmium (Cd) and 1 mgL-1 Cd-10 mgL-1 chitosan(CT) mixture on hematocrit levels, erythrocyte numbers and on erythrocyte and erythrocyte nucleus areas were determined after exposing the animals to Cd and Cd-CT mixture over 7, 15 and 30 days. Microhematocrit and microscopic methods were used in determining the above blood parameters. No mortality was observed during the experiments. Hematocrit levels and erythrocyte numbers decreased whereas erythrocyte and erythrocyte nucleus areas increased in fish exposed to Cd alone compared with the control. No difference was observed, however, in the parameters studied in Cd-CT exposed fish compared with the control, except the erythrocyte nucleus area (P
Effects of cadmium on sera glucose and cortisol levels in Clarias gariepinus (Burchell, 1822).
Karaytuğ, Sahire | Karayakar, Fahri | Çiftçi, Nuray | Cicik, Bedii | Ay, Özcan
The effects of cadmium on sera glucose and cortisol levels of Clarias gariepinus was studied after exposing the animals to 0.25, 0.50 and 1.00 ppm Cd over 30 days. An autoanalyser was used in determining sera parameters. Sera glucose levels showed no significant difference between the control and 0.25 and 0.50 ppm Cd exposed fish whereas 1.00 ppm cadmium decreased sera glucose significantly (p