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ANNALS OF MICROBIOLOGY3
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Fresenius Environmental Bulletin2
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Acta Microbiologica Polonica1
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Advances in Bioresearch1
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Annals of Microbiology1
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Bulletin of Environmental Contamination and Toxicology1
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Fen Bilimleri Öğretimi Dergisi1
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Folia Biologica (Krakow)1
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International Journal of Eurasia Social Sciences1
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International Journal of Social Sciences and Education Research1
Isolation of alcohol tolerant, osmotolerant and thermotolerant yeast strains and improvement of their alcohol tolerance by UV mutagenesis
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
In this study, the yeast strains were isolated from grapes by serial dilution technique to determine their alcohol-, sugar-and thermotolerance. 34 wild type yeast strains were isolated and alcohol-, sugar-and thermotolerance of these strains were determined. The maximum alcohol tolerance was found to be 9%(v/v) in yeast strain which is named Y2. Thermotolerance behavior of 6 strains were investigated. The strains were treated with UV light with intervals of 20, 30, 40 and 50 seconds. Selected resistant colonies were investigated for alcohol tolerance. It was found that alcohol tolerance increased from 9%(v/v) to 12%(v/v) on Y2 strain.
Isolation and characterization of lytic bacteriophages infecting fish pathogen Vibrio sp. strains
Talaş, Gizem | Coral, Gökhan | Coral, Mutlu Nisa Ünaldı | Yılmaz, Şakir Necat
In this study, bacteria were isolated from infected organs of sea bass (Dicentrarhus labrax) and identified as Vibrio sp. according to 16S rRNA analysis. Four bacteriophages were isolated from seawater samples collected from the coast of Mersin province in Turkey.Vibrio sp. strains were used as hosts for bacteriophage isolation. Bacteriophages inhibit the growth of their hosts; Vibrio sp. K12, Vibrio sp. K11, Vibrio sp. L8, Vibrio sp. L5 which are potential bacterial pathogen of sea bass. Bacteriophages were characterized by Restriction Analysis (RA) and Transmission Electron Microscopy (TEM). Restriction Analysis showed that genomes of bacteriophages LP8, KP12, LP5 and KP11 are double stranded DNAs and the sizes of these genomes are 73, 55, 44 and 23 kb respectively. Only three out of pha...
Öğretmen Adaylarının Fen Bilimleri Eğitiminde Sanal Tasarım ve Üç Boyutlu Yazıcı Entegrasyonunun İncelenmesi
Koç, Adem | Coral, Mutlu Nisa Ünaldı
Fen bilimleri içeriğinde soyut kavramların oldukça yoğun olduğu bir öğrenme alanıdır. Konu ve kavramlara göre somutlaştırma işlemleri geleneksel laboratuvar ve fiziksel tasarım atölyelerinde gerçekleştirilebilse de, bu her zaman mümkün olamamakta ya da daha zor olabilmektedir. Bu sorunların üstesinden gelebilmek ve öğrenmede somutlaştırmanın önemine vurgu yapmak için, bu çalışma fen bilgisi öğretmen adaylarının katılımı ile soyut kavramların çoğunlukta olduğu Biyoloji III dersi kapsamında gerçekleştirilmiştir. Haftalık olarak ikişer ders saati şeklinde mühendislik tasarım süreci aşamalarına ve proje tabanlı öğrenme yöntemine göre gerçekleştirilen bu uygulamada üç boyutlu tasarım programı olan Tinkercad programı ve üç boyutlu yazıcı kullanılmıştır. Uygulama süreci başında öğretmen adayları ...
Thermostable alkaline protease produced by an Aspergillus niger strain
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
A thermostable alkaline protease was isolated from the Aspergillus niger Z1 strain in a liquid Czapek Dox medium, containing casein (1% w/v) as the sole nitrogen source. Enzyme extract was subjected to electrophoresis in SDS-polyacrylamide gel. Two protein bands were seen on polyacrylamide gel. Active enzyme was visualized in a zymogram and protease activity exhibited a molecular mass of 68 kDa on SDS-polyacrylamide gel. The optimum pH for activity was found to be 9.0. The temperature optima of the enzyme was found to be 40 °C at pH 9.0 and it remained stable up to 90 °C, with 48.4% of the original activity retained after heat treatment at 90 °C for 15 min. Proteolytic activity was inhibited by PMSF, but slightly inhibited by SDS.
Incidence of inducible bacteriophage in Bacillus sp. And Pseudomonas sp. Strains isolated from contaminated soil in Mersin
Coral, Mutlu Nisa Ünaldı
In the current study, the effects of environmental agents on induction of soil lysogenic bacteria were studied. To estimate the prevalence of lysogeny within bacterial communities from contaminated soil, induction assays were carried out by extracting bacteria from soil. A series of experiments were conducted with samples collected in Mersin to assess the impact of some physical and chemical agents on propage induction in natural populations. A total of 90 bacteria were isolated from these sites. UV radiation, temperature, some mutagens and hydrocarbons were used to induce prophage in lysogenic bacteria. Two-thirds of the lysogenic bacteria showed prophage induction by one of the agents. The effects of these agents on the efficiency of plating (EOP) and on the adsorption of lytic bacteriop...
Electrofusion of Saccharomyces cerevisiae Auxotrophic Mutants of Identical Mating Type Using a Laboratory-Made System
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
Yeast protoplasts from haploid auxotroph strains DC6 (MATα) and FY73 (MATα) were exposed to an inhomogeneous alternating field (AC 200 V/cm). Due to the dielectrophoretic aggregation two or more cells with close membrane contact were formed between the two electrodes. The effects of different DC pulses were investigated to determine critical fusogenic pulse strength. In the results, the maximum fusant cells were obtained only when 8 kV field strengths were applied. These findings showed that the optimum fusogenic pulse strength for yeast protoplasts is 8 kV/cm under our experimental conditions. After fusion, the DNA contents of both hybrid and individual parental strains were compared. The amount of DNA in the hybrid was found to be about twofold higher than that in the individual parental...
Some properties of crude carboxymethyl cellulase of Aspergillus niger Z10 wild-type strain
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40 °C. It was found that the enzyme's activity has a broad pH range between 3 to 9 and 41.2% of the original activity was retained after heat treatment at 90 °C for 15 min.
Detection of polycyclic aromatic hydrocarbon (PAH) degradation genes in polluted soil by DNA extraction and colony hybridization
Coral, Gökhan | Coral, Mutlu Nisa Ünaldı
The aim of this study is to detect the DNA sequences for the PAH-dioxygenase gene, the key enzyme of the aeго-bic catabolic for polycyclic aromatic hydrocarbon degra-dation, in soil microorganisms by using extraction of total DNA, PCR amplification PAH-dioxygenase sequences, and detection with colony hybridization. To determine whether bacteria containing PAH-dioxygenase gene actually exist in environments contaminated by crude oil or not, the ge-nomic DNA was purified using a genomic DNA purifica-tion kit. Purified genomic DNA was then amplified with a thermal cycler, and the PCR products were separeted by agarose gel electrophoresis. Pseudomonas sp. strain ARP 26, which is capable of degrading phenanthrene, was used as target for colony hybridization. It was confirmed that Pseudomonas sp...
A preliminary study on tellurite resistance in Pseudomonas spp. isolated from hospital sewage
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
A total of 48 Pseudomonas spp., isolated from the Çukurova University Balcalı Hospital sewage in Adana, were characterized on the basis of morphological, cultural and biochemical characteristics. To improve our understanding of the ecology of tellurite resistance in bacteria, the minimum inhibition concentrations (MIC) of potassium tellurite (K2 TeO3 ) for growth were used to determine metal tolerance of the isolated strains. Most of the strains tolerated to 55 µgr/ml potassium tellurite. Ostrain Ps 37 tolerated relatively high concentrations of tellurite (80 µgr/ml). 27% of the strains possessed plasmid mediated tellurite resistance (Telr ).
FEN BİLİMLERİ ÖĞRETMENLERİNİN OKULDIŞI (DOĞA DENEYİMİNE DAYALI) EĞİTİME YÖNELİK GÖRÜŞLERİNİN DEĞERLENDİRİLMESİ
Coral, Mutlu Nisa Ünaldı
Bu çalışmanın amacı Fen Bilimleri Öğretmenlerinin okul dışı (doğa deneyimine dayalı) eğitime yönelik görüşlerini ortaya koymaktır.Araştırmanın çalışma grubunu elverişli örnekleme metoduyla belirlenen 20 fen bilimleri öğretmeni oluşturmaktadır. Veri toplama aracı olarak araştırmacı tarafından hazırlanan ve 6 açık uçlu sorudan oluşan yarı yapılandırılmış görüşme formu kullanılmıştır. Görüşmeler her bir öğretmenle yaklaşık yirmişer dakikalık oturumlar şeklinde gerçekleştirilmiş ve ses kaydı alınmıştır. Ayrıca araştırmacı görüşme boyunca görüşme formu üzerine de notlar almıştır.Elde edilen verilerin dijital ortama aktarılmasının ardından verilere içerik analizi uygulanmış, kodlar ve temalar belirlenmiştir. Elde edilen bu kod ve temalar çerçevesinde öğretmen görüşleri saptanmış ve değerlendiril...
Plasmid mediated heavy metal resistances in Enterobacter spp. isolated from Sofulu landfill, in Adana, Turkey
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
A total of 15 Enterobacter spp. were isolated from water drainage near the landfill in Sofulu village in Adana and were characterised on the basis of morphological, cultural and biochemical characteristics. The Maximum Tolerable Concentrations of cadmium, copper, chromium and nickel for growth were used to determine metal tolerance of the isolated strains. Among the resistant strains used in this study, Ent-5, Ent-7 and Ent-15 were found to have plasmid resistance to copper and nickel. One resistant strain, Ent-5, functioned as a donor of copper resistance. Copper resistance transferred from Ent-5 to Escherichia coli AB3505 at a frequency of approximetely 2.7 x 10-5 per recipient cell. Transformant strain had one plasmid with the same molecular weight donor strain’s plasmid. These isolates...
Effect of Domoic Acid on Growth and Heavy Metal Tolerance of Some Marine Bacteria Isolated from Samandag Shore and Iskenderun Bay
Coral, Mutlu Nisa Ünaldı
In the current study, effects of domoic acid on some marine bacteria were studied. Domoic acid is a neurotoxin which affects human and animals and by some marine microalgea. For this study, heterotrophic marine bacteria were isolated from Iskenderun Bay and Samandag shore. 19 Pseudomonas sp. strains, 15 Acinetobacter sp. strains, 5 Alteromonas sp. strains and 9 Vibrio sp. strains were identified. For inhibition tests, varing concentrations of domoic acid (0.5µgr.ml-1 and 1 mgr.ml-1) were tested. It was found that there is not any inhibition effect of domoic acid on the all bacteria with used concentrations. Stimulator effect was determined by getting growth curve from the bacteria cultures which was added varying concentrations of domoic acid. It was found that 2.5 µgr.ml-1 and 5 µgr.ml-1 ...
Development and Validation of the Metacognitive Awareness Scale for Learning with Multimedia
Koç, Adem | Coral, Mutlu Nisa Ünaldı
Technology has become increasingly prevalent in the field of education. Alongside the growing presence of technology in educational environments, the importance of learning with multimedia tools has also increased. It is believed that individuals need to be aware of how to learn most effectively in these environments for effective learning to take place. This research aims to develop a valid and reliable scale to reveal university students' metacognitive awareness of learning processes with multimedia. To achieve this goal, the study was conducted in two phases. Firstly, a pool of items was created by reviewing the literature, and 407 data were obtained to explore this pool of items. Subsequently, to confirm the structure of the revealed 6-factor scale, 318 data were collected from a secon...
Some properties of thermostable xylanase from an Aspergillus niger strain
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
A thermostable xylanase was isolated from an Aspergillus niger wild type strain in a liquid Czapek Dox medium, containing oat spelts xylan as the sole carbon source. The molecular mass of the enzyme was estimated to be about 36 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum pH for activity was found to be 7.5. The temperature optimum of the enzyme was found to be 60 °C at pH 7.5. The enzyme remained stable up to 100 °C, yet lost about 50% of its activity after 15 min at this temperature.
Keratinolytic activity of Streptomyces strain BA7, a new isolate from Turkey
Coral, Mutlu Nisa Ünaldı | Coral, Gökhan
A wild type Streptomyces strain BA7 which was isolated from soil, showed a high keratinolytic activity when cultured on native feather medium. Optimum keratinolytic activity was observed at 50 ºC and pH 8.5 when using fluid supernatant obtained from aerated culture of this organism. The keratinolytic activity was completely stable (100%) between 30 and 60 ºC. The molecular weight of crude enzyme was estimated by SDS-PAGE about 44000 Da. The degradation of intact feathers by Streptomyces sp. BA7 keratinase was obtained after 24 h of incubation at 50 ºC. Keratinolytic activity was partially inhibited by 1,10-phenanthroline, but slightly inhibited by CaCl2, ZnCl2, and SDS. In addition keratinolytic activity was enhanced by DMSO, EDTA, Triton-X 100 and sodium sulphite. Streptomyces strain BA7 ...
Potential neoplastic effects of parathion-methyl on rat liver
Coral, Mutlu Nisa Ünaldı
The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonella typhimurium TA100 bacterial cells was observed without rat liver S9 metabolic activation. Parathion-methyl was further investigated for pathological changes in rat pancreas and liver. The long-term rat experiments showed that parathion-methyl exposure for 3 months can cause pathological changes in rat pancreases acinar cells and pancreatic hepatocytes. Atypical acinar cell focuses (AACF) were determined in the liver and pancreas of the rats. The results from short-term Ames test and long-term rat experiments suggested that parathion-methyl would be potential carcinogenic.