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169357

Antioxidant supplementation ameliorates bull sperm parameters and fertilizing ability following the freeze-thaw process

TUNCER, Pürhan Barbaros | Büyükleblebici, Serhat

This study’s goal was to reveal the effects of antioxidant supplementation on motility, motion characters, morphology, DNA integrity, and fertilizing potential of cryopreserved bovine sperm. The ejaculates were collected from three Holstein bulls. At least ten ejaculates were collected per bull. The ejaculates were immediately separated into five aliquots, diluted in the Cryobos (Magapor Co. Ltd., Zaragoza, Spain) commercial extender, including 2.5 mM taurine, 2.5 mM cysteine, 2.5 mM methionine, 2.5 mM glutamine, and no additives were used. Afterward, they were frozen in 0.25 mL French straws, and liquid nitrogen was used for the storage of semen. The supplementation of methionine resulted in a higher subjective motility rate in comparison with the other groups. Taurine led to the lowest...

169361

The Comparison of Three Different Cryoprotectants in Cryopreservation of Angora Goat Semen

BÜYÜKLEBLEBİCİ, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga

The objective of this study was to evaluate glycerol (G), ethylene glycol (EG) and dimethylsulfoxide (DMSO) which were used two different doses on in vitro semen parameters, antioxidant enzymes activities and DNA damage after the freeze-thaw process in Angora goat semen. Semen samples from 5 mature Angora goats were used in this study. A total number of 40 ejaculates were collected twice a week from the goats using an artificial vagina and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 6 equal aliquots and diluted with tris base extenders supplemented with two different doses of cryoprotectants (G 3%, 6%; EG 3%, 6%; DMSO 3%, 6%). G 3% and 6% was added as a cryoprotectant had better CASA motility (P

169363

CAN LINOLEIC ACID IMPROVE THE QUALITY OF FROZEN THAWED BULL SPERM?

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici Olga

BACKGROUND: Cryopreservation is known to have a detrimental effect on the motility, viability and membrane integrity of sperm cells. OBJECTIVE: The aim of this study was to investigate the effect of various amount of linoleic acid supplementation to the Tris extender, on bull sperm parameters, DNA integrity and oxidative stress after freeze thawing. METHODS: Ejaculates were split into five aliquots and extended to a final concentration of 18x106 spermatozoa/ml with the base extender containing different doses of linoleic acid 0.125 ml, (L125); 0.250 ml (L250); 0.5 ml (L500), 1 ml (L1000) and no additive (control; L0). The extended samples were equilibrated slowly to 4°C for 4 h and then froze using a digital freezing machine. Frozen straws were thawed individually in water bath at 37°C...

169368

Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages

Tuncer, Purhan Barbaros | Buyukleblebici Serhat

The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): ≤3years, n: 20; middle age (M): 4–6 years, n: 20; old age (O): ≥7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5mg/120×106 CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48h. Later, the frozen straws were thawed in a wa...

169369

Genomewide association of male reproductive traits in Aksaray Malakli dogs

Tuncer, Pürhan Barbaros | Büyükleblebici Serhat

Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) geno type of the investigated characteristics and reproductive loci. A total of 46 male dogs aredividedintothreeagegroups(I≤3years,n = 19; II 4–6 years; n = 19, and III ≥7years,n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The A...

169391

Effects of different extenders and centrifugation/washing on postthaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm

Tuncer, P.B.

The main objective of the current study was to evaluate the effects of extender type and centrifugation/washing prior to cryopreservation on the postthaw sperm parameters, lipid peroxidation, and superoxide dismutase activity of Angora buck (Capra hircus ancryrensis) sperm. Ejaculates collected from three Angora bucks were used in this study. Two consecutive ejaculates from each buck were pooled and split into equal parts in four Falcon tubes. Two tubes were diluted at 37 8C and then centrifuged to remove semen plasma. After centrifugation, two sediment parts were diluted with a Tris-based extender and commercial Bioxcell extender, respectively. The remaining two parts, which were not centrifuged/washed, were diluted with the above-mentioned extenders, respectively. Diluted samples were...

169392

The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities

Tuncer, Pürhan Barbaros

Theaimofthisstudywastodeterminetheeffectsoftheantioxidantscurcumin,inositoland carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora goat semen. Ejaculates were collected via artificial vagina from three Angora goats and microscopically evaluated and ooledat37◦C. The pooled semen samples were diluted in aT ris-based extender, including curcumin (2.5, 5 or 10mM), inositol (2.5, 5 or 10mM), carnitine (2.5, 5 or 10mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2–0.3◦C/min) cooled to 5◦C and then cryopreserved in 0.25mL French straws. Frozen straws were thawed individually at 37◦C for 20s in a water bath, for microscopic sperm evaluation. The freezing extender supple...

169396

The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen

Tuncer, Pürhan Barbaros | Büyükleblebici, Serhat

The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose . Insemination doses were processed that each 0.25-mL straw contained 15 106 sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics...

169401

Effects of Some Cryoprotectants and Antioxidants on Frozen/Thawed Bull Sperm Motility and Motion Characteristics

P.B. Tuncer, M.N. Bucak, S. Büyükleblebici, F. Kuzugüden, S. Sarıözkan, U. Taşdemir, H. Kinet

The aim of the present study was to determinate the effects of different doses of glycerol and ethylene glycol in Tris extender containing the Trehalose or cystein on post-thawed bull sperm motility and motion charecteristics following cryopreservation.

169489

Değişik sulandırıcılar ile dondurulan aygır spermalarının in vitro değerlendirilmesi üzerine çalışmalar

Tuncer, P. B.

The aim of this research was to investigate spermatolojical charecteristics of stallion semen frozen with lactose and tris extenders. This experiment was done in the Anadolu State Farm and five Pure Arabian Stallions were used. Semen taken from five Arabian Stallions were extended in a way that to ensure 100x106 /cm3 motile spermatozoa by lactose and tris extenders and frozen in the macrotube in liquid nitrogen vapour. In the semen after thawing of 500C-40 seconds the average values of semen; sperm motility (%),total percentage of abnormal sperm (%), percentage of abnormal acrosome (%) and thermo-rezistance test motility (%) in 1., 2. and 3. hours were recorded; %47.44; %31.73; %22.64; %22.74; %9.13 and %2.58 respectively for frozen semen by lactose. In the semen after thawing of 500C-...

169506

Evaluationofpost-thawqualityofBrown-SwissandHolsteinbullsemendilutedwith different extenders

Tuncer,, Pürhan Barbaros

Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p

169348

Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga

There are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the d...

169370

The Effect of Green Tea Extract Supplementation in Bull Semen Cryopreservation

TUNCER, Pürhan Barbaros

This study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters in frozen thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containing different amounts of CT (5, 10, 25 and 50 μg/mL) and no-additive (control). All samples were equilibrated at 4°C for 4 h and were frozen using a digital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. Spermatozoa DNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipid peroxidation status was evaluated colorimetrically. CT supplementation did not impr...

169359

Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull

Tuncer, P. B. | Buyukleblebici S.

The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5 mM), hyaluronan (0.25, 1 mg ml 1) and fetuin (5,10 mg ml 1) in the freezing of Brown Swiss bull semen. The best percentagesof CASA motilities were achieved with 10 mg ml 1 of fetuin and 2.5 mM of cysteamine. For sperm morphology, 10 mg ml 1 of fetuin and 2.5 mM of cysteamine had better protective effects (P < 0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5 mg ml 1 of fetuin, were higher than those of the control group (P < 0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25 mg ml 1 of hyaluronan, and 2.5 and 7.5 mM of cysteamine led to lower rates o...

169482

The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4°C

Tuncer, P. B.

This study evaluated the protective effects of supplementation with three different sugars on the motility, morphology and DNA integrity of rat epididymal sperm chilled and stored at 4 C Epididymides were obtained from each donor. Rat epididymal sperm was diluted in Ham’s F10 plus raffinose, Ham’s F10 plus trehalose, Ham’s F10 plus fructose, and Ham’s F10 medium for control purposes. Thereafter, the extended sperm were chilled and stored in liquid form at 4 C. Sperm motility, morphological abnormalities and DNA damage were determined at 0 and 12h after chilling. No significant difference was observed in any of the parameters evaluated at 0 h, before storage (P > 0.05). After 12 h of storage, all sugar additives led to statistically higher motility, normal sperm morphology and DNA integrity...

169484

Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stres parameters of Angora goat semen

Tuncer, P. B.

This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation, and antioxidant activities of Angora goat semen after the freeze-thawing process.

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