The Effect of Green Tea Extract Supplementation in Bull Semen Cryopreservation
TUNCER, Pürhan Barbaros
This study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters in frozen thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containing different amounts of CT (5, 10, 25 and 50 μg/mL) and no-additive (control). All samples were equilibrated at 4°C for 4 h and were frozen using a digital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. Spermatozoa DNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipid peroxidation status was evaluated colorimetrically. CT supplementation did not impr...
Effects of Various Antioxidants on Cryopreserved Bull Sperm Quality
TUNCER,, Pürhan Barbaros | Büyükleblebici, Serhat | Büyükleblebici, Olga
The objective of this study was to assess the effects of antioxidant supplement (A), fetuin (F), aminoacid (AS) and cysteine (CY) on the sperm parameters, plasma membrane integrity, chromatin damage and antioxidant activities after freeze-thawing. Ejaculates were split into five aliquots and extended to a final concentration of 15x106 spermatozoa/ml with the Tris base extender containing 0.5 ml A, 2 mg/ml F, 13% AS, 5 mM CY and no additive (C). The extended samples were equlibrated slowly to 4°C during 4 h and then frozen using a digital freezing machine.. Frozen straws were thawed individually in water bath at 37°C for 30 s to analyse progressive motility and sperm motion characteristics as well as membrane integrity. Biochemical assays were performed in a spectrophotometer using commer...
The Effects of Deslorelin, GnRH Analogue with Long Acted on Sexual Activities in Angora Bucks
TUNCER, P. Barbaros
In this study, it was aimed to investigate the effect of deslorelin, a GnRH analogue -which can postpone the sexual activity in some male animals-on the levels of plasma testosteron, testes size and side effects in Angora Bucks. As a animal material, 12 Angora male goat aged between 2-5 years and provided by Lalahan Animal Research Center. Animal were divided two equal groups. Deslorelin implants were replaced under the ear skin of goats (Group I, n=6)and left for 15 days. The animals of control group (Group II, n=6) had placebo treatment. After removal of implants, testicular size and epididiymal and plasma testosteron levels were determined. Plasma testosteron levels of deslorelin (4.08±1.9 ng/ml) estimated on 3rd day before implant removal was significantly higher than the those of con...
The Comparison of Three Different Cryoprotectants in Cryopreservation of Angora Goat Semen
BÜYÜKLEBLEBİCİ, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga
The objective of this study was to evaluate glycerol (G), ethylene glycol (EG) and dimethylsulfoxide (DMSO) which were used two different doses on in vitro semen parameters, antioxidant enzymes activities and DNA damage after the freeze-thaw process in Angora goat semen. Semen samples from 5 mature Angora goats were used in this study. A total number of 40 ejaculates were collected twice a week from the goats using an artificial vagina and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 6 equal aliquots and diluted with tris base extenders supplemented with two different doses of cryoprotectants (G 3%, 6%; EG 3%, 6%; DMSO 3%, 6%). G 3% and 6% was added as a cryoprotectant had better CASA motility (P
Effect of α-Lipoic Acid Supplementation to Extender on Quality of Frozen-Thawed Bull Semen
BÜYÜKLEBLEBİCİ Olga | Büyükleblebici Serhat | Tuncer,, Pürhan Barbaros
This study was conducted to investigate the possible protective effect of α-lipoic acid (ALA) supplementation to extender on damages in the quality of bull semen elicited by freze-thawing process-induced oxidative stres. Ejaculates were collected via an artificial vagina from the bulls at once a week. Then they were split into five aliquots and extended with the Tris base extender containing different doses of ALA, except control. The extended samples were equilibrated slowly to 4°C for 4 h and then frozen using a digital freezing machine. Frozen straws were thawed to analyse progressive motility and sperm motility characteristics as well as plasma membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. Treatment of extender with ALA groups cause...