Genomewide association of male reproductive traits in Aksaray Malakli dogs

Tuncer, Pürhan Barbaros | Büyükleblebici Serhat

Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) geno type of the investigated characteristics and reproductive loci. A total of 46 male dogs aredividedintothreeagegroups(I≤3years,n = 19; II 4–6 years; n = 19, and III ≥7years,n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The A...

Interfacial characteristics of BIOfactor MTA and Biodentine with dentin

Şimşek, Emine

The objective of this study was to characterize the interface between dentin and MTA-Angelus (Angelus, Londrina, Brasil), Biodentine (Septodont, France) and BIOfactor MTA (Imicryl, Konya, Turkey) using scanning electron microscopy (SEM), energy disperse X-ray spectroscopy (SEM-EDS) and confocal laser scanning microscopy (CLSM). Fifteen dentin segments were obtained from previously extracted singlerooted human teeth. Canal lumens were instrumented with diamond burs and then randomly filled with MTA-Angelus, Biodentine or BIOfactor MTA and placed in distilled water or Hanks' Balanced Salt Solution (HBSS) for 28-days. The samples were examined with SEM and the thickness of the interfacial layer measured. SEM-EDS analysis was performed to determine principal elemental composition...

Stratification strength and light climate explain variation in chlorophyll a at the continental scale in a European multilake survey in a heatwave summer

Alp, Mehmet Tahir

To determine the drivers of phytoplankton biomass, we collected standardized morphometric, physical, and biological data in 230 lakes across the Mediterranean, Continental, and Boreal climatic zones of the European continent. Multilinear regression models tested on this snapshot of mostly eutrophic lakes (median total phosphorus [TP] = 0.06 and total nitrogen [TN] = 0.7 mg L−1), and its subsets (2 depth types and 3 climatic zones), show that light climate and stratification strength were the most significant explanatory variables for chlorophyll a (Chl a) variance. TN was a significant predictor for phytoplankton biomass for shallow and continental lakes, while TP never appeared as an explanatory variable, suggesting that under high TP, light, which partially controls stratification streng...

Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull

Tuncer, P. B. | Buyukleblebici S.

The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5 mM), hyaluronan (0.25, 1 mg ml 1) and fetuin (5,10 mg ml 1) in the freezing of Brown Swiss bull semen. The best percentagesof CASA motilities were achieved with 10 mg ml 1 of fetuin and 2.5 mM of cysteamine. For sperm morphology, 10 mg ml 1 of fetuin and 2.5 mM of cysteamine had better protective effects (P < 0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5 mg ml 1 of fetuin, were higher than those of the control group (P < 0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25 mg ml 1 of hyaluronan, and 2.5 and 7.5 mM of cysteamine led to lower rates o...

Association of serotonin transporter gene-linked polymorphic region and variable number of tandem repeat polymorphism of the serotonin transporter gene in lichen simplex chronicus patients with psychiatric status

Erdal, Mehmet Emin

Background The serotonin (5-hydroxytryptamine; 5-HT) is a key neurotransmitter in the central nervous system and a responsible mediator for the itch. Dysregulation of serotonergic pathways has been implicated in the pathogenesis of many complex neuropsychiatric diseases. Objectives The purpose of this study was to evaluate the relationship between lichen simplex chronicus and dysfunction and serotonin transporter (5-HTT) gene polymorphism. Methods Thirty-nine patients with lichen simplex chronicus and 61 healthy control subjects were examined. Results The results for the patients and control subjects were not significantly different ( P > 0.05) in long/long (L/L) and long/short (L/S) genotypes of 5-HTT gene-linked polymorphic region (HTTLPR) polymorphism, but short/short S/S genotype was l...

Metal-based additives "acetylferrocene and ruthenium polypyridyl complex" to improve performance and emission characteristics of ci engine

Resitoglu, Ibrahim Aslan

Metal-based fuel additives have been promising alternative choice to improve diesel engine performance and emission characteristics. Many studies have been performed on use of metal-based fuel additives such as magnesium, platinum, titanium, and cerium in diesel engine. Unlike conventional metal-based additives, Acetylferrocene and Ruthenium polypyridyl complex may be alternative metal-based additives for diesel. This study intends to investigate performance and emission variations of a diesel engine powered by diesel blended with Acetylferrocene and Ruthenium polypyridyl complex additives. In accordance with this purpose, a single cylinder, direct injection diesel engine was used to test these alternative metal additives. Results showed that Acetylferrocene and Ruthenium polypyridyl compl...

Changes in Optical Characteristics and Surface Topography of CAD/CAM Materials after Bleaching Applications: An AFM Evaluation

Cengiz Yanardag, Esra

Purpose: To evaluate the effect of in-office and at-home bleaching agents on color changes (ΔE00 ), translucency (TP00 ), whiteness (WID ), surface roughness (Ra ), and surface topography (Rsk and Rku ) of a resin nano-ceramic and a polymer-infiltrated ceramic network CAD/CAM material. Materials and methods: Sixty specimens (6 mm × 7 mm × 1.3 mm) were prepared from Lava Ultimate (LU) and Vita Enamic (VE). The specimens were divided into 6 groups according to the bleaching agents applied (n = 10/group): control, Perfect Bleach Office + (PBO), Opalescence Boost (OB), Perfect Bleach (PB) with 10% and 16% carbamide peroxide and Whiteness Perfect (WP). ΔE00 values were calculated before and after bleaching procedures with a spectrophotometer using the CIEDE2000 formula. ΔTP00 and ΔWID values w...

Transcutaneous Vagus Nerve Stimulation Enhances Probabilistic Learning

Büyükgüdük, İlkim | Geraldine Veldhuizen, Maria

tVNS enhances various memory and learning mechanisms, but there is inconclusive evidence on whether probabilistic learning can be enhanced by tVNS. Here, we tested a simplified version of the probabilistic learning task with monetary rewards in a between-participants design with left and right-sided cymba conchae and tragus stimulation (compared to sham stimulation) in a sample of healthy individuals (n = 80, 64 women, on average 26.38 years old). tVNS enhances overall accuracy significantly(p = 4.09 x 10⁻⁰⁴) and reduces response times (p = 1.1006 x 10⁻⁴⁹) in the probabilistic learning phase. Reinforcement learningmodelling of the data revealed that the tVNS group uses a riskier strategy, dedicates more time to stimulus encoding and motorprocesses and exhibits greater reward sensitivity re...

Removal of Basic Textile Dyes in Single and Multi-Dye Solutions by Adsorption: Statistical Optimization and Equilibrium Isotherm Studies

Gözmen, Belgin | Turabik, Meral

The removal of three basic dyes by adsorption onto bentonite was investigated forsingle, binary, and ternary solutions in a batch system. Before and after dye adsorption,bentonite samples were analyzed by using X-ray fluorescence spectrometer, SEM,and Fourier transform IR spectrometry. The D-optimal design and response surfacemethodology were applied in designing the experiments for evaluating the interactiveeffects of each initial concentrations variable of the dyes in binary systems. Predictedvalues were found to be in good agreement with experimental values, which definedpropriety of the model and the achievement of D-optimal in optimization of adsorptionof binary dye systems. The competitive adsorption results showed that the adsorptionamount of a dye was suppressed in the presence and i...

Red pine (Pinus brutia Ten) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

Tuncer,, Pürhan Barbaros

This study aimed to investigate the effectiveness of using red pine bark tree extract (P;Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200,100,50 and 25µg/ml) and P free (control ; C) up to a final concentration of 16×106 per straw. All specimens were equilibrated for a period of 4h rata temperature of 4°C ,following which they were filledin 0.25-ml French straws and frozen. Addition of P resulted in favourable tai llength incomparison with C (p.05). Inconclusion, it has been shown that although P added to a Tris extender does not have a pos...

Electroanalytical Investigation and Simultaneous Determination of Etodolac and Thiocolchicoside at a Non-Modified Glassy Carbon Electrode in Anionic Surfactant Media

In this study, a differential pulse voltammetry method was designed for the simultaneous determination of etodolac and thiocolchicoside in a tablet dosage form. This method is a sensitive, costless and reproducible one initially designed for the simultaneous determination of etodolac and thiocolchicoside using bare/unmodified glassy carbon electrode in the presence of sodium dodecyl sulfate. At optimized conditions, the method showed linear responses with etodolac and thiocolchicoside concentration in the range of 1 mu M to 80 mu M. The limits of detection and quantification were calculated as 0.11 mu M and 0.38 mu M for etodolac and 0.20 mu M and 0.67 mu M for thiocolchicoside, respectively.

Discrimination-Based Continuous Traumatic Stress, PTSD and Chronic Pain in Syrian Refugees: A Moderated Mediation Analysis

Emre Han ALPAY

Purpose This study aimed to examine the relationships among continuous traumatic stressors (CTS), posttraumatic stress disorder (PTSD), somatic symptoms, and chronic pain in a sample of Syrian refugees. Specifically, we hypothesized that PTSD would mediate the association between discrimination-based CTS and chronic pain and that somatic symptoms would moderate the link between PTSD and chronic pain, such that higher somatic symptom levels would amplify this association. Materials and Methods A total of 669 Syrian refugees residing in Türkiye participated in the study. Data were collected through face-to-face interviews using validated self-report instruments, including the Patient Health Questionnaire-15 (PHQ-15), the PTSD Checklist for DSM-5 (PCL-5), the Short-Form McGill Pain Questio...

Optimization of solvent extraction process of some Turkish coals using response surface methodology and production of ash-free coal

Gözmen, Belgin | Sönmez, Özgür

In order to produce ash-free coal (AFC) from three Turkish coals, solvent extraction of coals using 1-methylnaphthalene (1-MN) at different temperatures were conducted. The extraction yield of coals was found dependenton coal, temperature, and solvent type. When there was usage of 1-MN as solvent, the extraction yield was low. However,on addition of N-methyl-2-pyryrolidone (NMP) or quinoline (QN) into 1-MN, the extraction yield increased. Following theextractions, we obtained AFC with less than 0.65% ash content for the three coals used in this study. The response surfacemethodology (RSM) was employed to investigate the solvent extraction conditions. The factors investigated were extractiontemperature and additional polar solvent ratio. The obtained results demonstrated that either excess ...

Reduction of Pollutant Emissions in Diesel Engines Through Metal-Based Fuel Additives and Aftertreatment Emission Control Technologies
Metal-Based Fuel Additives and Aftertreatment Emission Control Technologies

Resitoglu, Ibrahim Aslan | Sugozu, Banu

Pollutant emissions such as carbon monoxide (CO), hydrocarbons (HCs), nitrogen oxides (NOx), and particulate matter (PM) from diesel engines have serious adverse effects on both human health and the environment. Advanced post-engine emission control systems, such as the diesel oxidation catalyst (DOC) and selective catalytic reduction (SCR), have proven effective in substantially reducing or minimizing emissions of CO, HC, and NOx. Additionally, the use of metal-based fuel additives in diesel fuel has been widely studied and applied in practice to improve engine performance and optimize emission outcomes. The interaction between metal-based fuel additives and the performance of DOC and SCR systems has become a key area of research focus. This study investigates the impact of metal-based fu...

Effects of curcumin and dithioerythritol on frozen-thawed bovine semen

Tuncer, P. B.

The aim of this study was to determine the effects of curcumin and dithio erythritol added into bull semen extender on sperm parameters, lipid peroxida tion, total glutathione and antioxidant potential levels of bull spermatozoa following the freeze/thawing process. Twenty-seven ejaculates obtained from three bulls were included in the study. Each ejaculate that was splitted into five equal groups and diluted in a Tris-based extender containing curcumin (0.5 and 2 mm), dithioerythritol (0.5 and 2 mm) and no additive (control) was cooled to 5 C and frozen in 0.25-ml French straws. The extender supple mented with 0.5 mm dose of curcumin led to lower percentage of total abnor mality (20.40 ± 2.36%) when compared to the control (30.60 ± 1.47%, P < 0.05). Curcumin and dithioerythritol...

Comparison of Cryoprotective Effects of Lycopene and Cysteamine in Different Cryoprotectants on Bull Semen and Fertility Results

Tuncer, P. B. | Büyükleblebici, S. | Büyükleblebici, O.

The objectives of this study were to compare glycerol and ethylene glycol at different concentrations as cryoprotectants and lycopene or cysteamine (with/without) as antioxidants in Tris extender for bull semen. Twenty-four ejaculates were obtained from three bulls. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and added using both of the cysteamine 5 mM or lycopene 500 lg/ ml, and control (without additives). The addition of 7% glycerol with cysteamine, 5% ethylene glycol with cysteamine and 3% ethylene glycol with cysteamine groups gave the lowest CASA motility than the other groups. However, 7% glycerol and 7% glycerol ...