Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER | YALIN, SERAP

In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.

Gökkuşağı alabalığı Oncorhynchus mykiss W Yemlerinde ham soya unu ve kavrulmuş soya kullanımının balıkların büyüme performansı yem tüketimi yemden yararlanma oranı ve besi maliyeti üzerine etkisi

Bilgüven, Murat | Barış, Mustafa

Effects of Chromium on the Hematocrit Levels and Erythrocyte Numbers of Cyprinus carpio

Cicik, Bedii | Çiftçi, Nuray | Ay, Özcan

Changes in hematocrit levels and erythrocyte numbers of Cyprinus carpio were determined after exposing the animals to 0.5, 1.0 and 2.0 ppm chromium over 7, 15 and 30 days. Microhematocrit and hemocytometric methods were used for determining hematocrit levels and erythrocyte numbers respectively. No mortality was observed during the experiments. Hematocrit levels and erythrocyt numbers increased under the effect of metal on day 15. Hematocrit levels and erythtrocyte numbers decreased by increasing exposure periods except at 0.5 ppm chromium.

The effects of freshwater rearing on the whole body and muscle tissue fatty acid profile of the European sea bass (Dicentrarchus labrax)

HUNT, ARZU ÖZLÜER | YILMAZ, FERBAL ÖZKAN | ENGİN, KENAN

The purpose of this study was to investigate the effect of freshwater rearing on the fatty acid profiles of the whole body and muscle tissue of the European sea bass (Dicentrarchus labrax). Half of initial fish were gradually acclimated to freshwater (FW) kept at the same temperature to salt water and grown in same conditions as their counterparts in saltwater (SW). The decrease in salinity caused an increase in the percentages of 18:1n − 9, 24:1n − 9, 18:3n − 3, 18:2n − 6 and decrease in the percentages of 14:0, 15:0, 20:0, 21:0, 20:5n − 3 and 22:6n − 3 both in the whole body and in the muscle tissue fatty acid profiles. The lipids of FW-reared fish contained significantly (P < 0.01) higher percentages of 18:2n − 6 and 18:3n − 6 than that of SW-reared fish. However, percentages of 20:5n −...

The protective role of ferulic acid against imidacloprid-induced oxidative stress in liver and brain of Cyprinus carpio

YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

This study evaluated the potential protective effect of ferulic acid (FA) on stress induced by imidacloprid (IM) in Cyprinus carpio (mean weight 60.10±2.22 g). Therefore, C. carpio was exposed to sublethal IM concentration (2.80 mg/L) for 10 days. During the experimental period, fish were fed a basal (control) diet and a basal diet supplemented with 5 g/kg of ferulic acid. The fish were divided into four groups (n = 6 in each group). The control group was exposed to tap water (the absence of imidacloprid) fed with control diet. The FA group was exposed to tap water (the absence of IM) and was fed with FA supplemented diet. The liver and brain tissues of both control and treated fish were dissected. Tissues samples were obtained from an individual fish and prepared for analysis. The variat...

Prophylactic efect of myricetin and apigenin against lipopolysaccharide‑induced acute liver injury

KARAYAKAR, FAHRİ | YILMAZ, FERBAL ÖZKAN | HUNT, ARZU ÖZLÜER

Background: Liver has an important role in the initiation and progression of multiple organ failure that occurs in sepsis. Many natural active substances can be used to reduce the liver injury caused by sepsis. For this aim, the efects of myricetin and apigenin on mice model of acute liver injury was evaluated in this study. Methods and results: Thirty-six mice were randomly divided into six groups as; control, lipopolysaccharide (LPS) (5 mg/kg), LPS+myricetin (100 mg/kg), LPS+myricetin (200 mg/kg), LPS+apigenin (100 mg/kg), and LPS+apigenin (200 mg/kg) groups. Myricetin and apigenin were administered orally for 7 days, and LPS was administered intraperitoneally only on the 7th day of the study. 24 h after LPS application, all animals were sacrifced and serum biochemical parameters, hist...

Metal(loid) accumulation and ecotoxicological assessment in cartilaginous fishes from the Northeastern Mediterranean

Çiftçi, Nuray | AYAS, Deniz

The present study was conducted with the objective of ascertaining the levels of selected metal(loid)s in the muscle, liver, spleen, kidney, stomach, intestinal, and gonad tissues of seven distinct cartilaginous species sampled from Northern Cyprus. This initiative was undertaken to address the dearth of metal bioaccumulation data for cartilaginous species and to elucidate the impact of metal pollution on these species, which are distinguished by their limited reproductive capacity. The samples were obtained from discarded species captured during commercial fishing activities. The metal concentrations in these tissues were subsequently determined using Inductively Coupled Plasma Mass Spectrophotometry (ICP-MS). The results indicated that the kidney tissue of Squalus blainville exhibited th...

New Record of the Velvet Belly Lanternshark Etmopterus spinax (Linnaeus, 1758) in the Deep Seas of Northern Cyprus

AYAS, Deniz | Çiftçi, Nuray

Lantern sharks are small shark species that can be seen at depths between 70 and 2000 meters. Due to their luminescent characteristics, they have been called “Lantern shark”. In total eleven specimens of the velvet belly lantern shark, Etmopterus spinax (Linnaeus, 1758), were caught in the deep seas of Northern Cyprus by using a bottom trawl. Sampling was carried out using 13 trawling operations. The collected samples were placed in 4% formalin and stored at the Museum of the Systematic, Faculty of Fisheries, Mersin University, (catalogue number: MEUFC-18-11-082). As a sampling area, the depths between 274 and 641 m were selected. Other cartilaginous fish caught during sampling except E. spinax were Galeus melastomus (1 individual), Squalus acanthias (4 individuals), Scyliorhinus ca...

Cranial Morphometrics of a Stranded Common Bottlenose Dolphin Tursiops truncatus (Montagu, 1821) in Mersin Bay (Northeastern Mediterranean, Turkey)

AYAS, Deniz | Çiftçi, Nuray

On 10 November 2017, a young male common bottlenose dolphin Tursiops truncatus (Montagu, 1821), 185 cm in total length (TL), was found dead on the Mersin Marina (36°46'065"N, 34°34'212"E) in Northeastern Mediterranean coast of Turkey. Measurements of the sample performed and documented. The specimen buried to obtain the skeleton of the individual. At the end of this process, the sample was deposited in the Museum of the Systematic, Faculty of Fisheries, Mersin University, (Catalogue no: MEUMC-17- 11-001). The morphometric measurements can contribute to ongoing fisheries biology and taxonomic studies of T. truncatus from Mediterranean Sea waters. During routine fishing activities, common bottlenose dolphins can be caught in fishing nets and drowned. This issue leads to an extra pop...

First Record of the Pempheris rhomboidea (Kossmann Räuber, 1877) from Mersin Bay, Northeastern Mediterranean Sea

Çiftçi, Nuray | Ayas, Deniz

In this study, eight individuals of Pempheris rhomboidea (Kossmann & Räuber, 1877) were for the first time caught in the Mersin Bay, Northeastern Mediterranean Sea. Some morphometric and meristic characters of these individuals were measured. The average standard length (SL): 90.13 mm, total length (TL): 112.62 mm, the length of longest pectoral fin (LPF): 25.88 mm, the length of longest dorsal fin ray (LDF): 21.25 mm, SL/LPF: 3.88, SL/LDF: 4.24, A: III, 39-42; D: VI, 9; LL pore scales: 55-58. The ratio of eye diameter (ED) to standard length (SL) is 7.95. The presence of the non-indigenous Pempheris rhomboidea was reported for the first time from Northeastern Mediterranean Sea and second time fromTurkish marine waters.

BAKIR, ÇİNKO, KURŞUN ve KADMİYUMUN Oreochromis niloticus' da BAZI SERUM BİYOKİMYASAL PARAMETRELER ÜZERİNE ETKİLERİ

Çiftçi, Nuray | Karayakar, Fahri | Ay, Özcan | Cicik, Bedii

Araştırmada bakır (Cu), çinko (Zn),kurşun (Pb) ve kadmiyum (Cd)’un 96 saatlik LC50 değerinin %10’unakarşılık gelen sırasıyla 4.0, 6.0, 0.2 ve 1.6 ppm ortam derişimlerinin 7, 15 ve30 gün sürelerle etkisine bırakılan Oreochromis niloticus’un serumglukoz, total protein, albumin, D-Bilirubin, aspartat aminotransferaz (AST),alanin aminotransferaz (ALT) ve total kolesterol düzeylerinde meydana gelendeğişimler belirlenmiştir. Denemesüresince incelenen derişimlerin etkisinde mortalite gözlenmemiştir. İncelenenmetallerin belirlenen süreler sonundaki etkisinde kontrole oranla, totalprotein, albumin ve D-Bilirubin düzeylerinde önemli bir ayırım saptanmazken,serum glukoz, AST, ALT ve total kolesterol düzeylerinde artış gözlenmiştir.Elde edilen bulgular, ağır metal etkisinin serum biyokimyasal parametr...

Effects of cadmium on sera glucose and cortisol levels in Clarias gariepinus (Burchell, 1822).

Karaytuğ, Sahire | Karayakar, Fahri | Çiftçi, Nuray | Cicik, Bedii | Ay, Özcan

The effects of cadmium on sera glucose and cortisol levels of Clarias gariepinus was studied after exposing the animals to 0.25, 0.50 and 1.00 ppm Cd over 30 days. An autoanalyser was used in determining sera parameters. Sera glucose levels showed no significant difference between the control and 0.25 and 0.50 ppm Cd exposed fish whereas 1.00 ppm cadmium decreased sera glucose significantly (p

Additional record of Trypauchen vagina (Bloch & Schneider, 1801) from Mersin Bay, Turkey.

Çiftçi, Nuray | Ayas, Deniz

Two mature individuals of Trypauchen vagina were caught by trawl fishing at 30 m depth in the Mersin coastal areas in September 2018. Meristic and morphometric measurements of the individuals were done and compared with its measurements reported in other studies. In previous studies; one record from Mersin Bay and in totally three records of T. vagina in the Northeastern Mediterranean Sea were reported. In this study, the second record of T. vagina was reported from Mersin Bay.

Alabalık karma yemlerinde tam yağlı soyanın balık ununun yerine kullanılma olanakları

Bilgüven, Murat

Balık unu salmonid yemlerinin en önemli hayvansal protein kaynağıdır. Ancak, fiyatının yüksek oluşu, her zaman istenilen miktarlarda bulunmayışı, çok çeşitli balıklardan çok çeşitli yöntemlerle (şekillerde) elde edilmesi sonucu kalitesinin sabit olmaması ve saklama güçlükleri nedeniyle ülkemizde alabalık karma yemlerine istenilen düzeyde balık unu katı1amamaktadır. Bundan dolayı bu araştırmada, alabalık rasyonlarında balık ununun bir kısmı yerine ülkemizde fiyatı balık unundan daha ucuz olan tam yağlı soya kullanılmasının, balıkların canlı ağırlık artısı, yem tüketimi, yemden yararlanma oranı ve ayrıca balık maliyetine etkisi incelenmiştir. Denemede ortalama canlı ağırlıkları 50.13 - 50.83 g arasında olan 600 adet gökkuşağı alabalığı (Salmo gairdneri, R.) gençleri kullanılmış ve deneme 10 ...

Accumulation and Elimination of Cadmium by the Nile Tilapia (Oreochromis niloticus) in differing Temperatures and Responses of Oxidative Stress Biomarkers

Karayakar, Fahri | Cicik, Bedii

The aims of this study were to investigate the accumulation (15 days) and elimination (15 and 30 days) of cadmium (Cd) in the liver, gill, kidney and muscle of Oreochromis niloticus following exposures to different concentrations (1 and 2 mg/L) of Cd at different water temperatures (20, 25, 30 o C). Additionally, responses of oxidative stress biomarkers (superoxide dismutase, SOD; catalase CAT; glutathione peroxidase, GPx and malondialdehyde, MDA) of the liver were determined following Cd exposures. In accumulation period, Cd levels increased significantly in all the tissues at all temperatures and tissue accumulation order was kidney>liver>gill. All tissues, except the muscle, accumulated Cd in relation to exposure concentrations and water temperatures. There was no measurable level of Cd...

First Report on the Elemental Composition of the Bigeye Thresher Shark Alopias superciliosus Lowe, 1841 from the Mediterranean Sea

Çiftçi, Nuray | Cicik, Bedii | Ayas, Deniz

Cartilaginous fish species have ecological importance. Besides, the ecotoxicological studies on these species are pretty insufficient. In this study, Al, Cr, Mn, Fe, Cu, Zn, As, Pb, Cd, and Sr levels were determined in muscle, liver, gill, kidney, spleen, stomach, and gonad tissues of Alopias superciliosus (Female, 240 cm TL) caught from Mersin Bay. Tissue metal analysis was determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). A statistical difference was found among the tissues in terms of the metals. Fe was determined to be the highest level in all tissues (pGill>Spleen>Gonad>Kidney>Stomach>Muscle. Zn was detected at higher levels in the liver and stomach and As in other tissues after Fe. Al has the highest level after Zn and As and was mainly found in the gills. ...