Evaluation of glucose metabolism and reproductive hormones in polycystic ovary syndrome on the basis of peroxisome proliferator-activated receptor (PPAR)-g2 Pro12Ala genotype
Tok, Devrim | Erdal, Mehmet Emin
BACKGROUND: Peroxisome proliferator-activated receptor (PPAR)-g2 Pro12Ala polymorphism has been suggested as a protective factor for polycystic ovary syndrome (PCOS). In this study, we aimed to investigate metabolic features and reproductive hormones in women with PCOS and compare these features with control women on the basis of Pro12Ala genotype. METHODS: This study involved 60 randomly selected women with PCOS and 60 controls. Main outcome measures were anthropometric measures, variables of glucose metabolism and reproductive hormones. All the patients were genotyped for Pro12Ala variant of PPAR-g2 gene. RESULTS: Patients with Pro12Ala polymorphism were more obese in both groups. Furthermore, they had lower fasting insulin levels, were less insulin-resistant and were less glucose-intole...
Association of Gly972Arg variant of insulin receptor substrate-1 with metabolic features in women with polycystic ovary syndrome
Tok, Devrim | Erdal, Mehmet Emin
Objective: To examine the prevalence and the effects of Gly972Arg (G972A) variant of insulin receptor substrate-1 (IRS-1) in women with polycystic ovary syndrome (PCOS). Design: Controlled clinical study. Setting: An academic clinical research center. Patient(s): Sixty women with PCOS and 60 control women matched for age. Intervention(s): Biometric measures, metabolic and hormonal measures, genetic analyses. Main Outcome Measure(s): Serum androgens, glucose, and insulin were measured. Blood leukocytes were used for genetic analyses. Result(s): The G972A variant was encountered more often in women with PCOS. The G972A carriers were more obese than their counterparts, had higher fasting insulin levels, and were more insulin-resistant. However, androgen levels did not differ on the basis of I...
Orak hücre hastalığı olan çocuklarda kararlı durum ve kriz dönemlerinde serum YKL40 ile diğer inflamatuar belirteçler arasındaki ilişki
Akbey, Veysi
ÖZET ORAK HÜCRE HASTALIĞI OLAN ÇOCUKLARDA KARARLI DURUM VE KRİZ DÖNEMLERİNDE SERUM YKL40 İLE DİĞER İNFLAMATUAR BELİRTEÇLER ARASINDAKİ İLİŞKİ Amaç: Orak hücre hastalığının patogenezinde inflamatuar belirteçlerin rolünün araştırıldığı birçok çalışma yapılmıştır. Biz bu çalışmada; inflamatuvar bir hastalık olan orak hücre hastalığında; kararlı durum ve damar tıkayıcı kriz dönemlerinde, serum YKL-40 ile diğer inflamatuar belirteçler olan CRP,IL-6,TNF-α düzeyleri arasındaki ilişkiyi araştırmayı amaçladık. Gereç ve yöntemler: Çalışmaya 38 orak hücre hastalıklı çocuk ve 38 sağlam çocuk dahil edildi. Hasta grubundan tam kan sayımı, C-reaktif protein (CRP), interlökin-6 (IL-6), tümör nekrozis faktör-alfa (TNF-α), YKL-40, kontrol grubundan ise tam kan sayımı CRP ve YKL-40 için kan örnekleri alındı ...
Endoplasmic Reticulum Stress in Myometrial Smooth Muscle Cells and Spontaneous Contraction Changes in the Uterus of Dehydroepiandrosterone-induced Polycystic Ovary Syndrome Rats
Savas Aktas | Ismail Un | Ibrahim Omer Barlas
Myometrial changes in polycystic ovary syndrome (PCOS) are poorly investigated. Thus, we aimed to investigate endoplasmic reticulum (ER) stress in myometrial smooth muscle cells and changes in spontaneous uterine contraction in PCOS. Twenty-one female Sprague-Dawley rats (21 days old) were divided into control (n = 7), vehicle (n = 7) and PCOS (n = 7) groups. While the control group was not injected subcutaneously, the vehicle group was injected subcutaneously with sesame oil (0.2 ml/day) for 20 consecutive days. The PCOS group was injected subcutaneously with dehydroepiandrosterone (6 mg/100 g/day dissolved in 0.2 ml sesame oil) for 20 consecutive days. Blood samples were collected for the measurement of follicle stimulating-hormone (FSH), luteinizing hormone (LH), testosterone (T),...
Polikistik over sendromlu hastalarda karaciğer yağlanması ve non-invaziv hepatıp indekslerin değerlendirilmesi
Akkurd, Derviş Murat.
ÖZET AMAÇ: Bu çalışmada Polikistik Over Sendromu (PKOS) hastalarında karaciğer yağlanmasının ultrasonografi ve hepatik non-invaziv indekslerle araştırılması ve metabolik parametreler ve biyokimyasal ölçümlerle korelasyonun ortaya konulması amaçlanmıştır MATERYAL VE METOT: Çalışmaya yeni tanı konmuş 33 PKOS hastası ve 29 gönüllü katılımcı dahil edildi. Hastaların detaylı tıbbi öyküsü alınarak boy, kilo ve bel çevresinden oluşan antropometrik ölçümleri ile sistolik ve diastolik kan basıncı ölçümleri yapıldı. Katılımcıların 12 saat açlıktan sonra alınan venöz kan örneklerinde açlık kan şekeri, insülin düzeyleri, açlık kolesterol seviyeleri (total kolesterol, düşük dansiteli lipoprotein (LDL), ve yüksek dansiteli lipoprotein (HDL), trigliserid, alanin aminotransferaz (ALT), aspartate aminotran...
The importance of IRS-1 Gly972Arg polymorphism in evaluating the response to metformin treatment in polycystic ovary syndrome
Tok, Devrim | Erdal, Mehmet Emin
BACKGROUND: Recent evidence suggests that one of the modes of action of metformin may be through phosphorylation of the insulin receptor and insulin receptor substrates. With this in mind, we supposed that the G972A variant of insulin receptor substrate-1 (IRS-1) may modulate the response to metformin treatment in women with polycystic ovary syndrome (PCOS). METHODS: This preliminary study involved 60 randomly selected women with PCOS. All patients received dietary instructions and metformin 500mg three times daily for 6 months. Main outcome measures were androgen levels, parameters of glucose and insulin metabolism and anthropometric variables. After a second evaluation of the patients at 6 months, they were genotyped for the Gly972Arg variant of the IRS-1 gene. RESULTS: Metformin had dif...
Endoplasmic Reticulum Stress in Myometrial Smooth Muscle Cells and Spontaneous Contraction Changes in the Uterus of Dehydroepiandrosterone-induced Polycystic Ovary Syndrome Rats
Aktaş, Savaş | Barlas, İbrahim Ömer | Ün, İsmail
Myometrial changes in polycystic ovary syndrome (PCOS) are poorly investigated. Thus, we aimed to investigate endoplasmic reticulum (ER) stress in myometrial smooth muscle cells and changes in spontaneous uterine contraction in PCOS. Twenty-one female Sprague-Dawley rats (21 days old) were divided into control (n = 7), vehicle (n = 7) and PCOS (n = 7) groups. While the control group was not injected subcutaneously, the vehicle group was injected subcutaneously with sesame oil (0.2 ml/day) for 20 consecutive days. The PCOS group was injected subcutaneously with dehydroepiandrosterone (6 mg/100 g/day dissolved in 0.2 ml sesame oil) for 20 consecutive days. Blood samples were collected for the measurement of follicle stimulating-hormone (FSH), luteinizing hormone (LH), testosterone (T), estra...