Bulunan: 15 Adet 0.001 sn
Yazarlar Tuncer,, Pürhan Barb ...
169658

Can Plant Extracts Be Used as an Alternative for Short- and/or Long-Term Storage of Semen in Some Animal Species?

Tuncer,, Pürhan Barbaros

Ex situ koruma, genetik kaynakların ve nesli tükenmekte olan kanatlıların korunmasında önemli bir yöntemdir. Gamet koruması için kanatlılarda tek yöntem spermanın dondurulmasıdır. Çünkü kanatlı yumurtaları oldukça hacimlidir ve bunların dondurularak saklanması için kullanılan kriyoprotektanların çözüm sonu uzaklaştırılması pek mümkün değildir. Son yirmi yılda, standart kanatlı spermasının don durma teknolojisini geliştirmek için birçok çalışma yapılmıştır. Farklı ırklara uyum sağlamak için birçok girişim ve yöntem araştırılmıştır. Bu bölümde, ilk olarak spermatozoanın yapısı ve kanatlı spermasının dondurulmasını etkileyen ana faktörlerden bahsedilmiştir. Nanopartiküller ve sperma sulandırıcılarına katılan nanopartiküllerin sperma dondurulabilirliği üzerine etkileri hakkında bilgi verilmiş...

169361

The Comparison of Three Different Cryoprotectants in Cryopreservation of Angora Goat Semen

BÜYÜKLEBLEBİCİ, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga

The objective of this study was to evaluate glycerol (G), ethylene glycol (EG) and dimethylsulfoxide (DMSO) which were used two different doses on in vitro semen parameters, antioxidant enzymes activities and DNA damage after the freeze-thaw process in Angora goat semen. Semen samples from 5 mature Angora goats were used in this study. A total number of 40 ejaculates were collected twice a week from the goats using an artificial vagina and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 6 equal aliquots and diluted with tris base extenders supplemented with two different doses of cryoprotectants (G 3%, 6%; EG 3%, 6%; DMSO 3%, 6%). G 3% and 6% was added as a cryoprotectant had better CASA motility (P

169506

Evaluationofpost-thawqualityofBrown-SwissandHolsteinbullsemendilutedwith different extenders

Tuncer,, Pürhan Barbaros

Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p

169348

Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici, Olga

There are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the d...

169655

The Role of Exogenous Antioxidants in Enhancing Reproductive Function and Performance

Tuncer,, Pürhan Barbaros

THE ROLE OF EXOGENOUS ANTIOXIDANTS IN ENHANCING REPRODUCTIVE FUNCTION AND PERFORMANCE IN HORSES Primary sex organs in males are the two testicles, where sperm are produced. The testicular seminiferous tubule and peritubular tissue are composed of germinal epithelium, and this tissue contains the basic cell types: somatic and germinal cells. The spermatogonial stem cells and germ cells, including differentiated cells formed during and after meiosis at dissimilar developmental degrees, are primary and secondary spermatocytes and spermatids, respectively.

169376

Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen

Tuncer,, Pürhan Barbaros

This study aimed to reveal the effects of juniper (Juniperus communis) berry oil (JB) when added to the Tris extender, on bull (Holstein) sperm motility characteristics, abnormal sperm ratio, DNA fragmentation and some oxidative stress markers. The pooled ejaculate was divided into five equal parts as control (C) and Tris diluent containing 25, 50, 100, 200 μg/ml JB. Diluted semen was incubated at 4 °C for 4 h and then frozen in liquid nitrogen (−196 °C). After freezing-thawing, motility characteristics were determined using a sperm analyzer system. The results showed that the 25 μg/ml dose of JB had an improving effect on overall sperm motility (P < 0.05). The total abnormal spermatozoa ratio was not significantly different between the groups (P > 0.05). The JB25 and JB50 groups had highe...

169373

Cryoprotective effect of vaccenic acid supplemented in bull semen extender

Tuncer,, Pürhan Barbaros

The aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 µg / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 °C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by using a programmable freezer. The greatest progressive and total motility results were obtained in the VA25 group (p < 0.001). Besides, the total abnormality and chromatin damage was determined to be on the lowest level in the VA25 gr...

169353

Cryopreservation of bull sperm: Effects of extendersupplemented with different cryoprotectants andantioxidants on sperm motility, antioxidant capacity and fertility results

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros

The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations and trehalose (T) or cysteine (C; with/without) in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was divided into four equal aliquots and diluted using both of the Tris extenders with G (5% or 7%) or EG (3% or 5%). After that, each extenders were divided into three equal aliquots and diluted using both of the 5 mM C or 25 mM T, and control (without additives) was cooled to 4◦C and frozen in 0.25 ml French straws.The addition of 3% and 5% EG without antioxidants resulted in the least Computer-Assisted Sperm motility Analysis (CASA) motility as compared with the other groups. Treatm...

169364

Effect of α-Lipoic Acid Supplementation to Extender on Quality of Frozen-Thawed Bull Semen

BÜYÜKLEBLEBİCİ Olga | Büyükleblebici Serhat | Tuncer,, Pürhan Barbaros

This study was conducted to investigate the possible protective effect of α-lipoic acid (ALA) supplementation to extender on damages in the quality of bull semen elicited by freze-thawing process-induced oxidative stres. Ejaculates were collected via an artificial vagina from the bulls at once a week. Then they were split into five aliquots and extended with the Tris base extender containing different doses of ALA, except control. The extended samples were equilibrated slowly to 4°C for 4 h and then frozen using a digital freezing machine. Frozen straws were thawed to analyse progressive motility and sperm motility characteristics as well as plasma membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. Treatment of extender with ALA groups cause...

169507

Rasyon çinko miktarının boğalarda sperma miktar ve kalitesine etkisi Effect of Diatery Zinc Level on Volume and Quality of Bull Semen

Tuncer,, Pürhan Barbaros | Büyükleblebici Serhat

The project was conducted in Livestock Central Research Institute. In the experiment 9 head Holstein bulls were used as project material. Bulls were split into groups of three heads as one control (40 mg/kg) and 2 different levels of zinc groups (150 mg/kg, group 1; 200 mg/kg, group 2) randomly by drawing of lots. Bulls in the control group were fed with compound feed containing 40 mg/kg zinc and bulls in two other treatment groups were given additional 110 mg/kg (Group1) and 160 mg/kg (Group 2) zinc respectively. As zinc preparation in control and other two treatment groups, organic zinc compound, Bioplex Zinc (zinc chelate of amino acid hydrate by Alltech) was used. After 60 days of a preliminary feeding period, semen was collected as 2 ejaculates at each time in 2 times a week during ...

169355

The Effects of Different Antioxidants on Post-thaw Microscopic and Oxidative Stress Parameters in the Cryopreservation of Brown-Swiss Bull Semen

Büyükleblebici, Olga | Tuncer,, Pürhan Barbaros | Büyükleblebici, Serhat

The aim of this study was to research the effects of four antioxidants that had been added to semen diluents, on spermatological parameters, anti-oxidant enzymes activities and DNA integrity after the freeze-thaw procedures in Brown-Swiss bull semen. A total of 24 ejaculates were collected from three bulls. Each ejaculate was divided into five equal parts and they diluted to the Tris + 25 mM trehalose base extender containing fetuin 1 mg/ml, dithioerithritol 1 mM, cysteamine 4 mM or linoleic acid 0.5 ml/50 ml, and no additives (control). Group dithioerithritol and linoleic acid showed the higher rates of CASA progressive motility, CASA sperm motility (P

169656

Kanatlı spermasının kısa ve uzun süreli saklanmasında ozmotik ile oksidatif stresin önlenmesi ve antioksidan kullanımı

Tuncer,, Pürhan Barbaros

Ex situ preservation is an important method of chicken preservation and the only method for gamete preservation is freezing of semen. In the last two decades, a lot of work has been done to improve the standard chicken semen freezing technology. Many attempts and methods have been investigated to adapt to different breeds. In this article, the structure of spermatozoa and the main factors affecting the freezing of poultry semen are first mentioned. In particular, an attempt has been made to summarize studies on important aspects of chicken semen freezing technology, including seperma diluents, cryoprotectants, packaging types and freezing and thawing rates. Information on the underlying mechanisms of freezing damage and freezability as revealed by recent osmotic and oxidative stress and ...

169657

Cryopreservation Of Stallion Semen

Tuncer,, Pürhan Barbaros

The 19th century saw the beginning of research on mammalian reproductive control, gamete cells in male and female animals, cryopreservation, semen collection and analysis, and artificial insemination (AI) in several animal species. Ivanoff initiated the first horse research in Russia. Subsequently, scientists from various nations have studied assisted reproductive technology (ART) in bees and other animal species. They have also examined substances and mechanisms that prevent oxidative stress and freezing diluted semen using various techniques. Researchers have previously studied sperm extraction, evaluation of semen, semen extenders, and various chemicals added to extenders that protect semen against freezing damage. Semen is utilized either fresh, chilled or, to a lesser extent, fr...

169363

CAN LINOLEIC ACID IMPROVE THE QUALITY OF FROZEN THAWED BULL SPERM?

Büyükleblebici, Serhat | Tuncer,, Pürhan Barbaros | Büyükleblebici Olga

BACKGROUND: Cryopreservation is known to have a detrimental effect on the motility, viability and membrane integrity of sperm cells. OBJECTIVE: The aim of this study was to investigate the effect of various amount of linoleic acid supplementation to the Tris extender, on bull sperm parameters, DNA integrity and oxidative stress after freeze thawing. METHODS: Ejaculates were split into five aliquots and extended to a final concentration of 18x106 spermatozoa/ml with the base extender containing different doses of linoleic acid 0.125 ml, (L125); 0.250 ml (L250); 0.5 ml (L500), 1 ml (L1000) and no additive (control; L0). The extended samples were equilibrated slowly to 4°C for 4 h and then froze using a digital freezing machine. Frozen straws were thawed individually in water bath at 37°C...

169371

Red pine (Pinus brutia Ten) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

Tuncer,, Pürhan Barbaros

This study aimed to investigate the effectiveness of using red pine bark tree extract (P;Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200,100,50 and 25µg/ml) and P free (control ; C) up to a final concentration of 16×106 per straw. All specimens were equilibrated for a period of 4h rata temperature of 4°C ,following which they were filledin 0.25-ml French straws and frozen. Addition of P resulted in favourable tai llength incomparison with C (p.05). Inconclusion, it has been shown that although P added to a Tris extender does not have a pos...