Evaluation of Biochemical Parameters in Coronary Artery Patients with Covid-19 Infection

Yaroğlu, Hatice Yıldırım | Yeşil, Emrah

As a result of pneumonia brought on by coronavirus disease (COVID-19), acute myocardial infarction, and chronic cardiovascular system damage, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells via ACE2 receptors. In patients with coronary artery disease (CAD) who are positive for COVID-19, early measurement of cardiac damage through biomarkers and careful monitoring of myocardial damage that may be caused by infection should be performed. The goal of this study was to retrospectively assess the results of biochemical laboratory testing in patients with CAD and COVID-19. Biochemical laboratory results of 70 patients with CAD and COVID-19 and 70 patients only with CAD were examined retrospectively. A signifcant difference was detected between groups in terms ...

The investigation of lipoprotein-related phospholipase A2 (LP-PLA2) V279F mutation in coronary artery disease

Yaroğlu, Hatice Yıldırım | Yılmaz, Dilek Çiçek | Tamer, Lülüfer

Objective: Despite advances in the treatment of cardiovascular diseases, coronary artery disease (CAD) maintains to be the leading cause of death in many nations. The media layer of normal and diseased arteries contains lipoprotein-associated phospholipase A2 (Lp-PLA2), which is mostly made by mast, T lymphocytes, and monocytes. Our research sought to define how the Lp-PLA2 and V279F gene polymorphisms relate to CAD. Materials and Methods: This study included 71 controls and 109 patients. Fasting blood glucose (FBG) and lipid profiles, were measured the enzymatic colorimetric technique. Lp-PLA2 levels were detected with ELISA. The genomic DNAs were isolated from whole blood. Real-time polymerase chain reaction (RT-PCR) with the V279F mutation detection kit was used to identify the Lp-PLA2 ...

The effects of interleukin (IL)1, 6, 10 and tumor necrosis factor (TNF) gene polymorphisms on CRP levels in coronary artery disease

Ünal, Zeynep Nil | Yaroğlu, Hatice Yıldırım | Yılmaz, Dilek Çiçek | Tamer, Lülüfer

Atherosclerosis is a dynamic and progressing inflammatory pathology. Studies have demonstrated that genetic variants that directly or indirectly alter the inflammatory system raise the risk of coronary artery disease (CAD). Genetic variations account for the variances in how the disease develops and progresses as well as the disclosure of poor prognostic information linked to extreme inflammatory reactions in individuals. In our investigation, we aimed to determine whether there may be a connection between CAD and the IL-1 C-889T, IL-6 G-174C, IL-10 G-1082A, and TNF G-308A polymorphisms. In the study, 80 patients with coronary artery stenosis of 70% or more and 80 people with normal coronary arteries were both evaluated using coronary angiography. An enzymatic colorimetric approach was use...

The role of CYP2C9 gene polymorphism in rheumatoid arthritis

Yaroğlu, Hatice Yıldırım | Biçer, Ali

Background/Aim: The inflammatory disorder rheumatoid arthritis (RA) affects quality of life and worsens with symptoms in the extra-articular tissues and systemic joints. The most significant member of the Cytochrome P450 enzyme family, Cytochrome P450 2C9 (CYP2C9), plays an essential role in the alkylation, demethylation, and hydroxylation of a variety of substances. Insufficient studies as to whether the susceptibility to rheumatoid arthritis is genetic exists. Therefore, our study presents new information on whether CYPC9 is a genetic risk factor. In this study, we sought to determine whether rheumatoid arthritis and the CYP2C9 gene polymorphism are related. Methods: This study was conducted as a prospective case-control study. Fifty patients with RA and 50 healthy individuals were inclu...

The relationship between ghrelin and ghrelin leu72met polymorphism in coronary artery disease

Purpose: Ghrelin represents a hormone, which is defined as an endogenous ligand bound to the growth hormone-releasing hormone receptor (GHS-R1a). However, the mechanisms that underlie ghrelin’s impacts on cardiovascular diseases have not been completely detected. For this reason, we aimed to research the relationship between serum ghrelin and ghrelin gene polymorphism in coronary artery disease (CAD). Materials and Methods: The study group consisted of 88 patients diagnosed with a minimum of one coronary artery stenosis over 70%, and the control group comprised 81 individuals without coronary artery lesions. An autoanalyzer was used to analyze fasting blood glucose (FBG) and lipid parameter levels. Ghrelin levels were examined with an enzyme-linked immunosorbent assay (ELISA) kit. Results:...

Glutathione S-transferase gene polymorphism as a susceptibility factor in smoking-related coronary artery disease

Yaroğlu, Hatice Yıldırım | Çamsarı, Ahmet | Yılmaz, Dilek Çiçek | Sucu, Nehir | Aras, Nurcan | Tamer, Lülüfer

Coronary artery disease (CAD) is the leading cause of morbidity and mortality in the world, and cigarette smoking is a major contributing factor to the disease. Glutathione S-transferase (GST) enzyme is implicated in the detoxification of carcinogens present in tobacco smoke and consequent polymorphisms in this gene may confer susceptibility to cardiovascular disease if DNA damage is important in CAD. Therefore, we examined this question in a case-control study of subjects having coronary atheroma by angiography and with a past history of myocardial infarction (MI). The study population consists of 247 healthy controls and 148 consecutive patients who had undergone coronary angiography for suspicion of coronary artery disease. DNA was extracted from whole blood, and the GSTM1 and GSTT1 pol...

MicroRNAs Expression Profiles in Non-Small Cell Lung Cancer

Yaroğlu, Hatice Yıldırım | Balcı, Şenay | Tamer, Lülüfer | Ayan, Erhan | Ünal, Zeynep Nil

MicroRNAs (miRNAs) are a new endogenous small non-coding RNA family that arranges expression of a few genes related in normal development as well as human diseases such as cancer. miRNAs are developing as potential diagnostic and therapeutic markers with deregulated expression in various cancers including lung cancer. In this study, we aimed to find out miRNA expression profiles and to show the relationship between non-small cell lung cancer and microRNAs levels, if there is. In the present study, the expression profiles of 740 miRNA in plasma from 31 patients and 64 healthy subjects were evaluated using high-throughput real-time quantitative polymerase chain reaction. All statistical analyses were performed using the Biogazelle's qbase PLUS 2.0 software. Our results showed that expression...

Investıgatıon of the possıble role of Poly (Adp-Rıbose) polymerase pathway in Nıcotıne- Exposed Testıcular Damage

Hazır, Selin | Yaroğlu, Hatice Yıldırım | Bayram, Gül | Aşkın, Ali

Although known adverse effects of nicotine exposure on general health, it is largely consumed as cigarette smoking. Smoking has negative effects on the fertility in males; however, the molecular mechanisms affected by nicotine are largely unclear. In this study, we aimed to investigate the effect of nicotine on poly(ADP-ribose) polymerase (PARP) pathway in testicular damage. Twenty-four male C57BL/6J mice were arbitrarily categorized into three subgroups: control, sham (subcutaneous, 0.9% sterile saline), and nicotine (subcutaneous, 3 mg/kg/body weight/day) groups. After 14 days of twice-daily subcutaneous injections, the weights of the body and testes were measured. The levels of testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), cotinine (main metabolite of nicot...